Human TFF1/PS2 ELISA Kit PicoKine™

Human TFF1/Ps2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TFF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Independently reviewed in 1 review(s).

Product Info Summary

SKU: EK1232
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human TFF1/PS2 ELISA Kit PicoKine™

See all TFF1/pS2 products

SKU/Catalog Number

EK1232

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TFF1/Ps2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TFF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TFF1/PS2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1232)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E25-F84

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1232 is reactive to TFF1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1232 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1232-CAP Anti-Human TFF1 Pre-coated 96-well strip microplate 1
EK1232-ST Human TFF1 Standard 2 vials, 1 ng/tube
EK1232-DA Human TFF1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0520.2200.3210.5230.8661.2531.7222.013

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TFF1/pS2, Dilution ratio of 1:1, concentration in serum is around 50pg/ml.

Some articles we found to cite concentrations of TFF1/pS2 in samples: 21699780 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TFF1/PS2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3517743338193420
Standard deviation1.969.9129.872.161129.4
CV (%)5.6%5.6%6.9%5.7%5.7%7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TFF1/pS2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 135373840371.84.8%
Sample 217715415617416510.326.2%
Sample 343339441640941314.013.3%
*number of samples for each test n=16.

Gene/Protein Information For TFF1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04155

Gene ID

7031

Gene Name

TFF1

Full Name

Trefoil factor 1

Weight

9.15kDa

Alternative Names

BCEI; BCEIbreast cancer, estrogen-inducible sequence expressed in; Breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; D21S21; gastrointestinal trefoil protein pS2; hP1.A; HPS2; PNR-2; Polypeptide P1.A; Protein pS2; PS2; TFF1; trefoil factor 1; trefoil factor, BCE1, human pS2 induced by estrogen from human breast cancercell line M10HP1.A TFF1 BCEI, D21S21, HP1.A, HPS2, pNR-2, pS2 trefoil factor 1 trefoil factor 1|breast cancer estrogen-inducible protein|breast cancer estrogen-inducible sequence|gastrointestinal trefoil protein pS2|polypeptide P1.A|protein pS2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TFF1, check out the TFF1 Infographic

TFF1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TFF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1232

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1 Reviews For Human TFF1/PS2 ELISA Kit PicoKine™

0

"Competitively priced and worked"

Excellent

Source: Biocompare.com

"Competitively priced and worked"

--Lu Yang, UCSD, CMMW, GRADUATE STUDENT

Applications Protein level screen
Sample Cell culture media from MCF7 cells
Detection --

"The TFF1 ELISA kit from Boster was competitively priced and worked with cell culture media taken from MCF7 cells, a human mammary carcinoma which is well known for overexpression of the TFF1 protein. The kit is very sensitive, detecting TFF1 protein at levels of 10pg/mL. The kit is saturated about 1mg/mL, so in my case, a 1 to 100 dilution of cell culture media was needed in order to be in the linear range of the assay. Good ELISA kit for human TFF1 protein. Worked as advertised."

Verified customer

Submitted

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9 Customer Q&As for Human TFF1/PS2 ELISA Kit PicoKine™

Question

Can you confirm if the volume of Biotinylated antibody and Avidin-Biotin-Peroxidase Complex has been changed from 130ul to 100ul for the Human TFF1/Ps2 ELISA Kit PicoKine™ (EK1232)?

Verified customer

Asked: 2021-01-28

Answer

The volume of Biotinylated antibody and Avidin-Biotin-Peroxidase Complex have been changed from 130ul to 100ul. All future lots contains 100 ul for those two components. Our lab takes reasonable loss into account and provide more than 100ul and they are sufficient for users to finish the assay.

Boster Scientific Support

Answered: 2021-01-28

Question

Will the Human TFF1/Ps2 ELISA Kit PicoKine work for all sample types?

Verified customer

Asked: 2019-11-05

Answer

Yes, we guarantee the kit to work on the sample types that we have tested for and if target protein is present at a level that falls within the linear range of the ELISA kit detection range. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.

Boster Scientific Support

Answered: 2019-11-05

Question

What is the recommended dilution for serum samples using EK1232?

Verified customer

Asked: 2019-11-05

Answer

The recommended dilution for serum samples using the Human TFF1/Ps2 ELISA Kit PicoKine™ (EK1232) is 1:1.

Boster Scientific Support

Answered: 2019-11-05

Question

Can the EK1232 kit be used to detect TFF1 in Human urine samples?

Verified customer

Asked: 2019-10-23

Answer

Our lab technicians have not validated the Human TFF1/Ps2 ELISA Kit PicoKine EK1232 with urine samples, so we cannot guarantee that it would work. In theory, the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.

Boster Scientific Support

Answered: 2019-10-23

Question

Q: Can TFF1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-05-03

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-05-03

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

H. Singh

Verified customer

Asked: 2019-04-29

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-04-29

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

M. Puri

Verified customer

Asked: 2018-05-15

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-05-15

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

M. Krishna

Verified customer

Asked: 2017-02-23

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-02-23

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of TFF1 in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2015-09-17

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TFF1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2015-09-17

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