Anti-Mucin Gastric Antibody (Monoclonal, 45M1)

SKU MA1061
Size 100μg/vial
Reactivity Human, Rabbit, Rat
Clonality Monoclonal
Clone Number 45M1
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, ICC, WB

Overview

Product Name Anti-Mucin Gastric Antibody (Monoclonal, 45M1)
SKU/Catalog Number MA1061
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Mucin Gastric, mucin 5AC, oligomeric mucus/gel-forming (MUC5AC) detection. Tested with WB, IHC-P, ICC in Human;rabbit;rat. No cross reactivity with other proteins.
Cite This Product Anti-Mucin Gastric Antibody (Monoclonal, 45M1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1061)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Mucin from human ovarian cyst fluid.
Reactivity Human, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, rabbit, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, rabbit, rat, -
Western blot, 1-2μg/ml, Human, rabbit, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

Images And Assay Conditions

Anti- Mucin Gastric antibody, MA1061, IHC(P)
IHC(P): Human Gastric Cancer Tissue

Anti- Mucin Gastric antibody, MA1061, IHC(P)
IHC(P): Rat Gastric Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P98088
Gene Name MUC5AC
Protein Name Mucin-5AC
Tissue Specificity Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. .
Alternative Names Mucin-5AC ;MUC-5AC ;Gastric mucin ;Lewis B blood group antigen ;LeB ;Major airway glycoprotein ;Mucin-5 subtype AC, tracheobronchial;Tracheobronchial mucin ;TBM ;MUC5AC ;MUC5 ;
Subcellular Localization Secreted .
Molecular Weight 585570 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.
Research Areas Human, Rabbit, Rat

*You can search these to find other products in these research areas.
Background Mucin 5AC (Muc5AC) is a protein that in humans is encoded by the MUC5AC gene. Using real-time RT-PCR, Moehle et al. (2006) found that MUC5AC was highly expressed in adult trachea and stomach. Expression was lower in adult lung and weak in fetal lung, and no expression was detected in other tissues. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease. Moreover, Muc5ac is identified as a direct and critical mediator of resistance during intestinal nematode infection.

Other Recommended Resources

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Monoclonal clone# G2 antibody for MUCIN 5B/MUC5B detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MUCIN 5B/MUC5B information: Molecular Weight: 585570 MW; Subcellular Localization: Secreted ; Tissue Specificity: Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum.
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In stock
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MA1061
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

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Publications

Zhou J, Perelman Jm, Kolosov Vp, Zhou X. Mol Cell Biochem. 2013 May;377(1-2):75-85. Doi: 10.1007/S11010-013-1572-3. Epub 2013 Feb 8. Neutrophil Elastase Induces Muc5Ac Secretion Via Protease-Activated Receptor 2.

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
  • Q: What are some alternative names that could be used to describe this product?
    A: One other very common name is muc6 antibody
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