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Product Info Summary
| SKU: | MA1061 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rabbit, Rat |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1)
SKU/Catalog Number
MA1061
BM0054 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1) catalog # MA1061. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1061)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
45M1
Isotype
Mouse IgG1
Immunogen
Mucin from human ovarian cyst fluid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1061 is reactive to MUC5AC in Human, Rabbit, Rat
Observed Molecular Weight
200 kDa
Calculated molecular weight
585.6 kDa
Background of MUC5AC
Mucin 5AC (Muc5AC) is a protein that in humans is encoded by the MUC5AC gene. Using real-time RT-PCR, Moehle et al. (2006) found that MUC5AC was highly expressed in adult trachea and stomach. Expression was lower in adult lung and weak in fetal lung, and no expression was detected in other tissues. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease. Moreover, Muc5ac is identified as a direct and critical mediator of resistance during intestinal nematode infection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1061 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, rabbit, rat
Immunocytochemistry , 1μg/ml, Human, rabbit, rat, -
Western blot, 1-2μg/ml, Human, rabbit, rat
Positive Control
IHC: human colon cancer tissue, human colon cancer tissue, rat colon tissue
Validation Images & Assay Conditions
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IHC analysis of Mucin Gastric using anti-Mucin Gastric antibody (MA1061).
Mucin Gastric was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Mucin Gastric Antibody (MA1061) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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IHC analysis of Mucin Gastric using anti-Mucin Gastric antibody (MA1061).
Mucin Gastric was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Mucin Gastric Antibody (MA1061) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
IHC analysis of Mucin Gastric using anti-Mucin Gastric antibody (MA1061).
Mucin Gastric was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Mucin Gastric Antibody (MA1061) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Specific Publications For Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1) (MA1061)
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1 Customer Q&As for Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1)
Question
We are currently using anti-Mucin Gastric antibody (Monoclonal, 45M1) MA1061 for rabbit tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human, rabbit, rat. Is it true that the antibody can work on zebrafish tissues as well?
J. Kulkarni
Verified customer
Asked: 2014-07-14
Answer
The anti-Mucin Gastric antibody (Monoclonal, 45M1) (MA1061) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-07-14
