Anti-Myoglobin/MB Antibody Picoband™

Boster Bio Anti-Myoglobin/MB Antibody Picoband™ catalog # A04058. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A04058
Size: 100ug/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Myoglobin/MB Antibody Picoband™

See all Myoglobin products

SKU/Catalog Number

A04058

Size

100ug/vial

Form

Lyophilized

Description

Boster Bio Anti-Myoglobin/MB Antibody Picoband™ catalog # A04058. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Myoglobin/MB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04058)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Myoglobin (3-35aa LSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFK), different from the related mouse sequence by three amino acids, and from the related rat sequence by six amino acids.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A04058 is reactive to MB in Human

Applications

A04058 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For MB (Source: Uniprot.Org, NCBI)

Uniprot ID

P02144

Gene ID

4151

Gene Name

MB

Full Name

Myoglobin

Weight

17.184kDa

Superfamily

globin family

Alternative Names

MB; MGC13548; Myoglobin; PVALB MB PVALB myoglobin myoglobin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MB, check out the MB Infographic

MB infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A04058

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13 Customer Q&As for Anti-Myoglobin/MB Antibody Picoband™

Question

We are currently using anti-Myoglobin/MB antibody A04058 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

The anti-Myoglobin/MB antibody (A04058) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-27

Question

I have a question about product A04058, anti-Myoglobin/MB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-07

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04058 anti-Myoglobin/MB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-07

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-Myoglobin/MB antibody A04058. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-26

Question

Will A04058 anti-Myoglobin/MB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

As indicated on the product datasheet, A04058 anti-Myoglobin/MB antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-11-06

Question

Does anti-Myoglobin/MB antibody A04058 work for WB with heart?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

According to the expression profile of heart, MB is highly expressed in heart. So, it is likely that anti-Myoglobin/MB antibody A04058 will work for WB with heart.

Boster Scientific Support

Answered: 2019-07-26

Question

I see that the anti-Myoglobin/MB antibody A04058 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-10

Question

Is this A04058 anti-Myoglobin/MB antibody reactive to the isotypes of MB?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

The immunogen of A04058 anti-Myoglobin/MB antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Myoglobin (3-35aa LSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFK), different from the related mouse sequence by three amino acids, and from the related rat sequence by six amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-20

Question

I was wanting to use to test anti-Myoglobin/MB antibody A04058 on human heart for research purposes, then I may be interested in using anti-Myoglobin/MB antibody A04058 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

K. Kulkarni

Verified customer

Asked: 2018-10-09

Answer

The products we sell, including anti-Myoglobin/MB antibody A04058, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-10-09

Question

See below the WB image, lot number and protocol we used for heart using anti-Myoglobin/MB antibody A04058. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-08-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-08-14

Question

Do you have a BSA free version of anti-Myoglobin/MB antibody A04058 available?

J. Walker

Verified customer

Asked: 2014-10-30

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Myoglobin/MB antibody A04058 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-10-30

Question

Is a blocking peptide available for product anti-Myoglobin/MB antibody (A04058)?

C. Anderson

Verified customer

Asked: 2014-05-01

Answer

We do provide the blocking peptide for product anti-Myoglobin/MB antibody (A04058). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-05-01

Question

I am interested in using your anti-Myoglobin/MB antibody for response to hydrogen peroxide studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.

O. Anderson

Verified customer

Asked: 2014-04-11

Answer

Thanks for your inquiry. This A04058 anti-Myoglobin/MB antibody is tested on human hela, hepg2 whole cell lysate, hela whole cell lysate, jurkat whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2014-04-11

Question

We have observed staining in human heart right ventricle. Do you have any suggestions? Is anti-Myoglobin/MB antibody supposed to stain heart right ventricle positively?

B. Wu

Verified customer

Asked: 2014-01-07

Answer

From literature heart right ventricle does express MB. From Uniprot.org, MB is expressed in heart right ventricle, skeletal muscle, heart, among other tissues. Regarding which tissues have MB expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 7895732
Skeletal muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2014-01-07

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A04058
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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