Anti-Myosin phospho S19/phospho S20 MYL12A Antibody

MRCL3 antibody

Boster Bio Anti-Myosin phospho S19/phospho S20 MYL12A Antibody (Catalog # P11637-1). Tested in ELISA, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: P11637-1
Size: 100uL
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-Myosin phospho S19/phospho S20 MYL12A Antibody

View all MRCL3 Antibodies

SKU/Catalog Number

P11637-1

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Myosin phospho S19/phospho S20 MYL12A Antibody (Catalog # P11637-1). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Myosin phospho S19/phospho S20 MYL12A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P11637-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Clone Number

1B9

Isotype

Antiserum

Immunogen

Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P11637-1 is reactive to MYL12A in Human

Applications

P11637-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

19.794kDa

Background of MRCL3

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. This antibody is specific for the phosphorylated form of myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
IHC: 2.5 µg/ml
IP: 1:100
WB: 1:1,000 - 1:5,000

Validation Images & Assay Conditions

Gene/Protein Information For MYL12A (Source: Uniprot.org, NCBI)

Gene Name

MYL12A

Full Name

Myosin regulatory light chain 12A

Weight

19.794kDa

Alternative Names

MLCBMyosin RLC; MRCL3; MRLC3MLC-2B; MYL2B; myosin regulatory light chain 12A; Myosin regulatory light chain 2, nonsarcomeric; myosin regulatory light chain 3; myosin regulatory light chain MRCL3; Myosin regulatory light chain MRLC3; myosin, light chain 12A, regulatory, non-sarcomeric; myosin, light polypeptide, regulatory, non-sarcomeric (20kD); RLC MYL12A HEL-S-24, MLC-2B, MLCB, MRCL3, MRLC3, MYL2B myosin light chain 12A myosin regulatory light chain 12A|epididymis secretory protein Li 24|myosin RLC|myosin regulatory light chain 2, nonsarcomeric|myosin regulatory light chain 3|myosin regulatory light chain MRLC3|myosin, light chain 12A, regulatory, non-sarcomeric|myosin, light polypeptide, regulatory, non-sarcomeric (20kD)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MYL12A, check out the MYL12A Infographic

MYL12A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MYL12A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

P11637-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Glucagon-like peptide 1 reverses myocardial hypertrophy through cAMP/PKA/RhoA/ROCK2 signaling

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3 Customer Q&As for Anti-Myosin phospho S19/phospho S20 MYL12A Antibody

Question

We are currently using anti-Myosin phospho S19/phospho S20 antibody P11637-1 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

The anti-Myosin phospho S19/phospho S20 antibody (P11637-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-19

Question

Is a blocking peptide available for product anti-Myosin phospho S19/phospho S20 antibody (P11637-1)?

K. Moore

Verified customer

Asked: 2015-06-30

Answer

We do provide the blocking peptide for product anti-Myosin phospho S19/phospho S20 antibody (P11637-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-06-30

Question

I see that the anti-Myosin phospho S19/phospho S20 antibody P11637-1 works with WB, what is the protocol used to produce the result images on the product page?

J. Yang

Verified customer

Asked: 2013-10-11

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-10-11

Order DetailsPrice
P11637-1

100uL

$599

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