Anti-N Cadherin/CDH2 Antibody Picoband®

Western blot analysis of N Cadherin/CDH2 using anti-N Cadherin/CDH2 antibody (A01577-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-N Cadherin/CDH2 antigen affinity purified polyclonal antibody (Catalog # A01577-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for N Cadherin/CDH2 at approximately 150KD. The expected band size for N Cadherin/CDH2 is at 150KD.

IHC analysis of N Cadherin/CDH2 using anti-N Cadherin/CDH2 antibody (A01577-3).
N Cadherin/CDH2 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-N Cadherin/CDH2 Antibody (A01577-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IHC analysis of N Cadherin/CDH2 using anti-N Cadherin/CDH2 antibody (A01577-3).
N Cadherin/CDH2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-N Cadherin/CDH2 Antibody (A01577-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IHC analysis of N Cadherin/CDH2 using anti-N Cadherin/CDH2 antibody (A01577-3).
N Cadherin/CDH2 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-N Cadherin/CDH2 Antibody (A01577-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IF analysis of N Cadherin/CDH2 using anti-N Cadherin/CDH2 antibody (A01577-3).
N Cadherin/CDH2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-N Cadherin/CDH2 Antibody (A01577-3) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of N Cadherin/CDH2 using anti-N Cadherin/CDH2 antibody (A01577-3) and anti-Tubulin Alpha antibody (M03989-3).
N Cadherin/CDH2 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-N Cadherin/CDH2 Antibody (A01577-3) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Flow Cytometry analysis of HEPG2 cells using anti-N Cadherin/CDH2 antibody (A01577-3).
Overlay histogram showing HEPG2 cells stained with A01577-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-N Cadherin/CDH2 Antibody (A01577-3, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Western blot analysis of N Cadherin/CDH2 using anti-N Cadherin/CDH2 antibody (A01577-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human cervical cancer tissue lysates,
Lane 2: human cervical cancer adjacent tissue lysates,
Lane 3: human cervical cancer tissue lysates,
Lane 4: human cervical cancer adjacent tissue lysates.
Lane 5: human cervical cancer tissue lysates,
Lane 6: human cervical cancer adjacent tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-N Cadherin/CDH2 antigen affinity purified polyclonal antibody (Catalog # A01577-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for N Cadherin/CDH2 at approximately 140KD. The expected band size for N Cadherin/CDH2 is at 150KD.

Silibinin inhibited the growth and promoted the apoptosis of LA795 cells. (A) . Migration of LA795 cells in the presence of 0, 3, 10, 30, 100, and 300 μM silibinin assessed by wound healing assay ( n = 4). (B) . Statistical results of the percentage of wound area in (A) ( n = 4) (C) . Statistical results of LA795 cells viability incubated with different concentrations of silibinin ( n = 6). (D) . Expression of β -catenin, E-cadherin, N-cadherin, and vimentin of LA795 cells after treated with 200 μM silibinin for 24 h ( n = 3). (E) . Representative real-time images of LA795 cells incubated with different concentrations of silibinin ( n = 6). (F) . Representative images of apoptotic cells after 200 μM silibinin treatment for 24 h detected by annexin V apoptosis assay ( n = 3). (G) . Expression of cleaved-caspase 3 and cleaved-caspase 9 of LA795 cells after 200 μM silibinin treatment for 24 h ( n = 3).
Index in PubMed under a CC BY license. PMID: 33935737