|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-MMP-14 Antibody|
|Description||Rabbit IgG polyclonal antibody for Matrix metalloproteinase-14(MMP14) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-MMP-14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2147)|
|Replacement Item||This antibody may replace the following items: sc-101451|sc-12366|sc-12367|sc-12367-R|sc-26703|sc-26704|sc-30074|sc-373908|sc-377097 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14(567-582aa TPRRLLYCQRSLLDKV), different from the related mouse and rat sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Matrix metalloproteinase-14|
|Molecular Weight||65894 MW|
|Protein Function||Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro- MMP2. .|
|Tissue Specificity||Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. .|
|Sequence Similarities||Belongs to the peptidase M10A family.|
|Subcellular Localization||Membrane ; Single-pass type I membrane protein . Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm.|
|Alternative Names||Matrix metalloproteinase-14;MMP-14;188.8.131.52;MMP-X1;Membrane-type matrix metalloproteinase 1;MT-MMP 1;MTMMP1;Membrane-type-1 matrix metalloproteinase;MT1-MMP;MT1MMP;MMP14;|
|Research Areas|||cardiovascular|angiogenesis|adhesion / ecm|matrix metalloproteinases|mmp| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes| cancer|invasion/microenvironment|mmps| cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|tumor biomarkers|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolism processes|types of disease||
Background for Matrix metalloproteinase-14
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MMP-14 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MMP-14 Antibody Images
Click the images to enlarge.
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Placenta Tissue
IHC(P): Rat Cardiac Muscle Tissue
All lanes: Anti MMP14 (PA2147) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Predicted bind size: 66KD
Observed bind size: 150KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,