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Pack Size:100μg/vial
Validated Species:Human, Mouse, Rat
Application:IHC, WB
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Product Name Anti-MMP-14 Antibody
SKU/Catalog Number PA2147
Description Rabbit IgG polyclonal antibody for Matrix metalloproteinase-14(MMP14) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-MMP-14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2147)
Replacement Item This antibody may replace the following items: sc-101451|sc-12366|sc-12367|sc-12367-R|sc-26703|sc-26704|sc-30074|sc-373908|sc-377097 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14(567-582aa TPRRLLYCQRSLLDKV), different from the related mouse and rat sequences by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MMP14
Protein Name Matrix metalloproteinase-14
Molecular Weight 65894 MW
Protein Function Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro- MMP2. .
Tissue Specificity Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. .
Sequence Similarities Belongs to the peptidase M10A family.
Subcellular Localization Membrane ; Single-pass type I membrane protein . Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm.
Uniprot ID P50281
Alternative Names Matrix metalloproteinase-14;MMP-14;;MMP-X1;Membrane-type matrix metalloproteinase 1;MT-MMP 1;MTMMP1;Membrane-type-1 matrix metalloproteinase;MT1-MMP;MT1MMP;MMP14;
Research Areas |cardiovascular|angiogenesis|adhesion / ecm|matrix metalloproteinases|mmp| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes| cancer|invasion/microenvironment|mmps| cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|tumor biomarkers|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolism processes|types of disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Matrix metalloproteinase-14

Matrix metalloproteinase-14, also called MT-MMP 1, is an enzyme that in humans is encoded by the MMP14 gene. The protein encoded by this gene is a member of the membrane-type MMP subfamily. This gene is mapped to 14q11.2. Proteins of the matrix metalloproteinase(MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This gene may be involved in actin cytoskeleton reorganization by cleaving PTK7. It acts as a positive regulator of cell growth and migration via activation of MMP15.

Anti-MMP-14 Antibody Images

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Anti-MMP-14 Antibody
Anti-MMP14 antibody, PA2147, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-MMP-14 Antibody
Anti-MMP14 antibody, PA2147, IHC(P)
IHC(P): Human Placenta Tissue
Anti-MMP-14 Antibody
Anti-MMP14 antibody, PA2147, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Anti-MMP-14 Antibody
Anti-MMP14 antibody, PA2147, Western blotting
All lanes: Anti MMP14 (PA2147) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Predicted bind size: 66KD
Observed bind size: 150KD
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Zhou H, Wu A, Fu W, Lv Z, Zhang Z. Oncol Lett. 2014 May;7(5):1395-1400. Epub 2014 Feb 27. Significance Of Semaphorin-3A And Mmp-14 Protein Expression In Non-Small Cell Lung Cancer.
Dong Q, Yu D, Yang Cm, Jiang B, Zhang H. Int J Exp Pathol. 2010 Aug;91(4):368-73. Doi: 10.1111/J.1365-2613.2010.00724.X. Epub 2010 Jun 24. Expression Of The Reversion-Inducing Cysteine-Rich Protein With Kazal Motifs And Matrix Metalloproteinase-14...
Shi Ch, Wang Ws, Zhang Zd, Li Cj, Guo Fj, Li F, Chen Am. J Huazhong Univ Sci Technolog Med Sci. 2015 Feb;35(1):111-6. Doi: 10.1007/S11596-015-1398-1. Epub 2015 Feb 12. Effect Of Lentivirus-Mediated Upa Silencing On The Proliferation And Apoptosis ...
Xiao H, Li H, Yu G, Xiao W, Hu J, Tang K, Zeng J, He W, Zeng G, Ye Z, Xu H. Oncol Rep. 2014 Apr;31(4):1832-8. Doi: 10.3892/Or.2014.3048. Epub 2014 Feb 24. Microrna-10B Promotes Migration And Invasion Through Klf4 And Hoxd10 In Human Bladder Cancer.
Jin F, Jiang S, Yang D, Zhang X, Yang Y, Zhang Y, Li K, Yang Y, Ma S. Biochem Biophys Res Commun. 2012 Nov 9;428(1):86-92. Doi: 10.1016/J.Bbrc.2012.10.011. Epub 2012 Oct 8. Acipimox Attenuates Atherosclerosis And Enhances Plaque Stability In Apoe-...
Jiang S, Jin F, Li D, Zhang X, Yang Y, Yang D, Li K, Yang Y, Ma S. High Alt Med Biol. 2013 Jun;14(2):175-80. Doi: 10.1089/Ham.2012.1083. Intermittent Hypobaric Hypoxia Promotes Atherosclerotic Plaque Instability In Apoe-Deficient Mice.

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to mmp 14 antibody, mmp14 antibody, mmp-14 antibody, mt1 mmp antibody, mt1-mmp antibody