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- Table of Contents
MMP14 antibody (PA2147) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Anti-MMP14 antibody, PA2147, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
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Anti-Matrix metalloproteinase-14 MMP14 Antibody Picoband®
MMP14 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
MMP14 antibody was positively detected by WB in:
Rat Spleen Tissue, Human Placenta Tissue, HELA Whole Cell, A549 Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MMP14 antibody was positively detected by IHC in:
Human Intestinal Cancer Tissue, Human Placenta Tissue, Rat Cardiac Muscle Tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat, Mouse
Western blot
0.1-0.5μg/ml
Human, Rat, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Recommend
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Matrix metalloproteinase-14 MMP14 Antibody Picoband®
View all MMP14 Antibodies
View all MMP14 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14, different from the related mouse and rat sequences by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated Molecular Weight
65.9 kDa
Background of MMP14
Matrix metalloproteinase-14, also called MT-MMP 1, is an enzyme that in humans is encoded by the MMP14 gene. The protein encoded by this gene is a member of the membrane-type MMP subfamily. This gene is mapped to 14q11.2. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This gene may be involved in actin cytoskeleton reorganization by cleaving PTK7. It acts as a positive regulator of cell growth and migration via activation of MMP15.
Cite This Product
Anti-Matrix metalloproteinase-14 MMP14 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2147)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Zebrafish
100%
High Homology
Mouse
94%
High Homology
Rat
94%
High Homology
Rabbit
94%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Dog
94%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MMP14 antibody
Immunohistochemistry (IHC) protocol for MMP14 antibody
Publications
Application:
Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway
PMID: 29393353
The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-?1 pathway
PMID: 27453531
Zhang Jt, Sun W, Zhang Wz, Ge Cy, Liu Zy, Zhao Zm, Lu Xs, Fan Yz. Bmc Cancer. 2014 Mar 15;14:193. Doi: 10.1186/1471-2407-14-193. Norcantharidin Inhibits Tumor Growth And Vasculogenic Mimicry Of Human Gallbladder Carcinomas By Suppression Of The Pi...
PMID: 24628713
Zhou H, Wu A, Fu W, Lv Z, Zhang Z. Oncol Lett. 2014 May;7(5):1395-1400. Epub 2014 Feb 27. Significance Of Semaphorin-3A And Mmp-14 Protein Expression In Non-Small Cell Lung Cancer.
PMID: 24765144
Dong Q, Yu D, Yang Cm, Jiang B, Zhang H. Int J Exp Pathol. 2010 Aug;91(4):368-73. Doi: 10.1111/J.1365-2613.2010.00724.X. Epub 2010 Jun 24. Expression Of The Reversion-Inducing Cysteine-Rich Protein With Kazal Motifs And Matrix Metalloproteinase-14...
PMID: 20579139
Shi Ch, Wang Ws, Zhang Zd, Li Cj, Guo Fj, Li F, Chen Am. J Huazhong Univ Sci Technolog Med Sci. 2015 Feb;35(1):111-6. Doi: 10.1007/S11596-015-1398-1. Epub 2015 Feb 12. Effect Of Lentivirus-Mediated Upa Silencing On The Proliferation And Apoptosis ...
PMID: 25673203
Xiao H, Li H, Yu G, Xiao W, Hu J, Tang K, Zeng J, He W, Zeng G, Ye Z, Xu H. Oncol Rep. 2014 Apr;31(4):1832-8. Doi: 10.3892/Or.2014.3048. Epub 2014 Feb 24. Microrna-10B Promotes Migration And Invasion Through Klf4 And Hoxd10 In Human Bladder Cancer.
PMID: 24573354
Jin F, Jiang S, Yang D, Zhang X, Yang Y, Zhang Y, Li K, Yang Y, Ma S. Biochem Biophys Res Commun. 2012 Nov 9;428(1):86-92. Doi: 10.1016/J.Bbrc.2012.10.011. Epub 2012 Oct 8. Acipimox Attenuates Atherosclerosis And Enhances Plaque Stability In Apoe-...
PMID: 23058919
Jiang S, Jin F, Li D, Zhang X, Yang Y, Yang D, Li K, Yang Y, Ma S. High Alt Med Biol. 2013 Jun;14(2):175-80. Doi: 10.1089/Ham.2012.1083. Intermittent Hypobaric Hypoxia Promotes Atherosclerotic Plaque Instability In Apoe-Deficient Mice.
PMID: 23795739
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PA2147
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 19, 2026