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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-MMP2 Antibody Picoband™
Boster Bio Anti-MMP2 Antibody Picoband™ catalog # A00286. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00286)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human MMP2 recombinant protein (Position: K429-C660). Human MMP2 shares 95.7% and 95.3% amino acid (aa) sequence identity with mouse and rat MMP2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00286 is reactive to MMP2 in Human, Mouse, Rat
A00286 is guaranteed for ELISA, WB Boster Guarantee
Background of MMP-2
Matrix metalloproteinase-2 (MMP2) is a Type IV collagenase, 72-kD, which is also known as gelatinase and is a member of a group of secreted zinc metalloproteases. The MMP2 gene is 17 kb long with 13 exons varying in size from 110 to 901 bp and 12 introns ranging from 175 to 4,350 bp, located within a region of human chromosome 16q13. In addition, the extra exons encode the amino acids of the fibronectin-like domain which has so far been found in only the 72- and 92-kDa type IV collagenase. MMP2, which has a critical role in the binding of progelatinase A and TIMP4 via the C-terminal hemopexin-like domain (C domain), is functionally associated on the surface of angiogenic blood vessels. Not only is a likely effector of endometrial menstrual breakdown, MMP2 is also effector and regulator of the inflammatory response. Moreover, MMP2 could be helpful in diagnosing Takayasu arteritis.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MMP2 using anti-MMP2 antibody (A00286).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
lane 1: recombinant human MMP2 protein 1ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP2 antigen affinity purified polyclonal antibody (Catalog # A00286) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP2 at approximately 71KD. The expected band size for MMP2 is at 71KD.
Gene/Protein Information For MMP2 (Source: Uniprot.Org, NCBI)
72 kDa type IV collagenase
peptidase M10A family
72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 18.104.22.168; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) MMP2 CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1 matrix metallopeptidase 2 72 kDa type IV collagenase|collagenase type IV-A|matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|matrix metalloproteinase-2|matrix metalloproteinase-II|neutrophil gelatinase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MMP2, check out the MMP2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A00286 has been cited in 46 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Combination of ferulic acid, ligustrazine and tetrahydropalmatine inhibits invasion and metastasis through MMP/TIMP signaling in endometriosis
Mechanism of transmembrane and coiled‑coil domain 1 in the regulation of proliferation and migration of A549 cells
MicroRNA‑153 attenuates hypoxia‑induced excessive proliferation and migration of pulmonary arterial smooth muscle cells by targeting ROCK1 and NFATc3
Exogenous H2S mitigates myocardial fibrosis in diabetic rats through suppression of the canonical Wnt pathway
Decreased TIP30 Expression Promotes Tumor Metastasis in Lung Cancer
Nicotine Alters Morphology and Function of Retinal Pigment Epithelial Cells in Mice:
c-Myc induced upregulation of long non-coding RNA SNHG16 enhances progression and carcinogenesis in oral squamous cell carcinoma
Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathy
Inhibition effect of Guizhi-Fuling-decoction on the invasion of human cervical cancer
IL-17 Stimulates Migration of Carotid Artery Vascular Smooth Muscle Cells in an MMP-9 Dependent Manner via p38 MAPK and ERK1/2-Dependent NF-κB and AP-1 Activation
Integrated Transcriptomic and Proteomic Analyses of the Interaction Between Chicken Synovial Fibroblasts and Mycoplasma synoviae. X. Jian,Xiao Chunhong,C. Hongyan,Lu Huijun,Yu Haizhong,Y. Jianfen
A00286 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:NA
Nie L,Liu M,Chen J,Wu Q,Li Y,Yi J,Zheng X,Zhang J,Chu C,Yang J.Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway.Mol Med Rep.2021 Apr;23(4):299.doi:10.3892/mmr.2021.11938.Epub 2021 Mar 2.PMID:336498
A00286 usage in article: APP:WB, SAMPLE:VENTRICULAR TISSUE, DILUTION:1:500
Hua, D.,Tang, L.,Wang, W.,Tang, S.,Yu, L.,Zhou, X.,Wang, Q.,Sun, C.,Shi, C.,Luo, W.,Jiang, Z.,Li,H.,Yu, S.,Improved Antiglioblastoma Activity and BBB Permeability by Conjugation of Paclitaxel to a Cell‐Penetrative MMP‐2‐Cleavable Peptide. Adv.Sci.2020,200
A00286 usage in article: APP:WB, SAMPLE:U87MG CELL AND U251 CELL, DILUTION:1:1000
Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
A00286 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000
Anti-endometriosis Mechanism of Jiawei Foshou San Based on Network Pharmacology
Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1%u03B1 and autophagy in human osteosarcoma
Inactivation of lysyl oxidase by %u03B2-aminopropionitrile inhibits hypoxia-induced invasion and migration of cervical cancer cells
Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells
Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation
Association of MMP-2 expression and prognosis in osteosarcoma patients
TGF-?1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21
The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-?1 pathway
Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-?B activation
Ezrin contributes to cervical cancer progression through induction of epithelial-mesenchymal transition
Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-?B Pathway
MMP-1/2 and TIMP-1/2 expression levels, and the levels of collagenous and elastic fibers correlate with disease progression in a hamster model of tongue cancer
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An?in vitro?investigation
Amniotic Mesenchymal Stem Cells Can Enhance Angiogenic Capacity via MMPs In Vitro and In Vivo
Effect of Ginkgo biloba extract on experimental cardiac remodeling
Efficacy of intravitreal captopril on oxygen-induced retinopathy in mice
Inhibition of CXCR4 activity with AMD3100 decreases invasion of human colorectal cancer cells in vitro
Yang Y, Gao M, Lin Z, Chen L, Jin Y, Zhu G, Wang Y, Jin T. Oncotarget. 2017 Oct 17;8(58):98708-98722. doi: 10.18632/oncotarget.21864. eCollection 2017 Nov 17. DEK promoted EMT and angiogenesis through regulating PI3K/AKT/mTOR pathway in triple-neg...
Li C, Liu J, Zhang Q, Cui K, Ge Q, Wang C, Chen Z. Int J Oncol. 2018 Jun;52(6):1815-1826. doi: 10.3892/ijo.2018.4346. Epub 2018 Mar 29. Upregulation of E-cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder...
Zhang Q, Wang C, Miao S, Li C, Chen Z, Li F. Oncotarget. 2017 Sep 30;8(55):93969-93983. doi: 10.18632/oncotarget.21400. eCollection 2017 Nov 7. Enhancing E-cadherin expression via promoter-targeted miR-373 suppresses bladder cancer cells growth an...
Li P, Chen H, Chen S, Mo X, Li T, Xiao B, Yu R, Guo J. Br J Cancer. 2017 Feb 28;116(5):626-633. doi: 10.1038/bjc.2016.451. Epub 2017 Jan 12 Circular RNA 0000096 affects cell growth and migration in gastric cancer
Wang M, Li M, Yan P, Luo Q, Zhang Y, Du Z. J Ophthalmol. 2017;2017:6067890. doi: 10.1155/2017/6067890. Epub 2017 Feb 9. The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment
Zhang Jt, Sun W, Zhang Wz, Ge Cy, Liu Zy, Zhao Zm, Lu Xs, Fan Yz. Bmc Cancer. 2014 Mar 15;14:193. Doi: 10.1186/1471-2407-14-193. Norcantharidin Inhibits Tumor Growth And Vasculogenic Mimicry Of Human Gallbladder Carcinomas By Suppression Of The Pi...
Zhang Y, Shen Y, Cao B, Yan A, Ji H. Exp Ther Med. 2015 Mar;9(3):905-908. Epub 2014 Dec 19. Elevated Expression Levels Of Androgen Receptors And Matrix Metalloproteinase-2 And -9 In 30 Cases Of Hepatocellular Carcinoma Compared With Adjacent Tissu...
Liu J, Ping W, Zu Y, Sun W. Int J Clin Exp Pathol. 2014 Aug 15;7(9):6040-7. Ecollection 2014. Correlations Of Lysyl Oxidase With Mmp2/Mmp9 Expression And Its Prognostic Value In Non-Small Cell Lung Cancer.
Fu Y, Jiao Y, An N, Liang A. Cytotechnology. 2013 Aug;65(4):533-9. Doi: 10.1007/S10616-012-9518-2. Epub 2012 Dec 15. Pegfp-N1-Mediated Bmk Ct Expression Suppresses The Migration Of Glioma.
Sun Fl, Wang W, Cheng H, Wang Y, Li L, Xue Jl, Wang Xf, Ai Hx, Zhang L, Xu Jd, Wang Xm, Ji Xm. Plos One. 2014 Jun 30;9(6):E101194. Doi: 10.1371/Journal.Pone.0101194. Ecollection 2014. Morroniside Improves Microvascular Functional Integrity Of The ...
Li Jk, Yu L, Shen Y, Zhou Ls, Wang Yc, Zhang Jh. World J Gastroenterol. 2008 Apr 21;14(15):2308-13. Inhibition Of Cxcr4 Activity With Amd3100 Decreases Invasion Of Human Colorectal Cancer Cells In Vitro.
Zhang J, Chen X, Shi G, Xie X, Liu H, Zhang X, Lai Y, Zuo Y, Chen Z, Liu S, Wang H. J Ovarian Res. 2013 Feb 6;6(1):9. Doi: 10.1186/1757-2215-6-9. Establishment Of A New Representative Model Of Human Ovarian Cancer In Mice.
Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.
Fang L, Huang C, Meng X, Wu B, Ma T, Liu X, Zhu Q, Zhan S, Li J. Toxicol Appl Pharmacol. 2014 Oct 15;280(2):335-44. Doi: 10.1016/J.Taap.2014.08.006. Epub 2014 Aug 19. Tgf-??1-Elevated Trpm7 Channel Regulates Collagen Expression In Hepatic Stellate...
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