Anti-MMP2 Antibody Picoband®

Regulation of FLT on xenograft EMS. (A) Ectopic volume was detected by vernier caliper in xenograft EMS model. GTN was performed as positive control. (B) Weight of nude mice was measured every 7 days during 28 days’ treatment. * P < 0.05 to pretreatment. Columns, mean ( n = 6). (C–E) Ectopic endometrium of EMS and FLT groups were collected for RNA isolation. Eutopic endometrium of C3H mice without transplantation were supplied as the control group. mRNA levels of MMP-2, MMP-9, and TIMP-1 were detected by qPCR in different groups. (F–I) Protein levels of MMP-2, MMP-9, and TIMP-1 were measured by western blotting, and the ratio of MMP-2, MMP-9, and TIMP-1 with β-actin were shown. # P < 0.05 to control, ## P < 0.01 to control, * P < 0.05 to EMS, ** P < 0.01 to EMS. Columns, mean ( n = 3). Bars, SD. EMS, endometriosis; FLT, ferulic acid, ligustrazine and tetrahydropalmatine; GTN, gestrinone. Download full-size image DOI:
Index in PubMed under a CC BY license. PMID: 34249506

Gene expression of MMP/TIMP signaling adjusted by FLT for 24h. The mRNA levels of MMP-2, MMP-9, and TIMP-1 were detected by qPCR in hEM15A (A–C) and HEC1-B (D–F) cells. * P < 0.05 to control group, ** P < 0.01 to control group. Columns, mean ( n = 3). Bars, SD. FLT, ferulic acid, ligustrazine and tetrahydropalmatine. Download full-size image DOI:
Index in PubMed under a CC BY license. PMID: 34249506

Modification of MMP/TIMP signaling protein treating with FLT for 24h. Protein levels of MMP-2, MMP-9, and TIMP-1 were detected by western blot in hEM15A (A–D) and HEC1-B (E–H) cells. The ratio of MMP-2, MMP-9, and TIMP-1 with β-actin were shown. * P < 0.05 to control group, ** P < 0.01 to control group. Columns, mean ( n = 3). Bars, SD. FLT, ferulic acid, ligustrazine and tetrahydropalmatine. Download full-size image DOI:
Index in PubMed under a CC BY license. PMID: 34249506

Effect of JFS on invasion and metastasis. (A–C) The mRNA levels of MMP-2, MMP-9, and TIMP-1 were detected by qPCR in different groups. (D–G) The protein levels of MMP-2, MMP-9, and TIMP-1 were detected by western blotting, and the ratio of MMP-2, MMP-9, and TIMP-1 with β-actin were shown. # P < 0.05 to control, ## P < 0.01 to control, ∗ P < 0.05 to EMS, ∗∗ P < 0.01 to EMS. Columns, mean ( n = 3). Bars, SD. EMS, endometriosis; JFS, Jiawei Foshou San .
Index in PubMed under a CC BY license. PMID: 30093862

Western blot analysis of MMP2 using anti-MMP2 antibody (A00286).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: rat H9C2 whole cell lysates,
Lane 3: mouse 3T3-L1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP2 antigen affinity purified polyclonal antibody (A00286) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MMP2 at approximately 72 kDa. The expected band size for MMP2 is at 74 kDa.