Anti-MMP2 Antibody Picoband™

Boster Bio Anti-MMP2 Antibody Picoband™ catalog # A00286. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 35 publication(s).

Product Info Summary

SKU: A00286
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-MMP2 Antibody Picoband™

See all MMP-2 products

SKU/Catalog Number

A00286

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MMP2 Antibody Picoband™ catalog # A00286. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MMP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00286)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human MMP2 recombinant protein (Position: K429-C660). Human MMP2 shares 95.7% and 95.3% amino acid (aa) sequence identity with mouse and rat MMP2, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00286 is reactive to MMP2 in Human, Mouse, Rat

Applications

A00286 is guaranteed for ELISA, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For MMP2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P08253

Gene ID

4313

Gene Name

MMP2

Full Name

72 kDa type IV collagenase

Weight

73.882kDa

Superfamily

peptidase M10A family

Alternative Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) MMP2 CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1 matrix metallopeptidase 2 72 kDa type IV collagenase|collagenase type IV-A|matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|matrix metalloproteinase-2|matrix metalloproteinase-II|neutrophil gelatinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MMP2, check out the MMP2 Infographic

MMP2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MMP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00286 has been cited in 35 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Integrated Transcriptomic and Proteomic Analyses of the Interaction Between Chicken Synovial Fibroblasts and Mycoplasma synoviae. X. Jian,Xiao Chunhong,C. Hongyan,Lu Huijun,Yu Haizhong,Y. Jianfen
Species: Human
A00286 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:NA

Nie L,Liu M,Chen J,Wu Q,Li Y,Yi J,Zheng X,Zhang J,Chu C,Yang J.Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway.Mol Med Rep.2021 Apr;23(4):299.doi:10.3892/mmr.2021.11938.Epub 2021 Mar 2.PMID:336498
Species: Rat
A00286 usage in article: APP:WB, SAMPLE:VENTRICULAR TISSUE, DILUTION:1:500

Hua, D.,Tang, L.,Wang, W.,Tang, S.,Yu, L.,Zhou, X.,Wang, Q.,Sun, C.,Shi, C.,Luo, W.,Jiang, Z.,Li,H.,Yu, S.,Improved Antiglioblastoma Activity and BBB Permeability by Conjugation of Paclitaxel to a Cell‐Penetrative MMP‐2‐Cleavable Peptide. Adv.Sci.2020,200
Species: Human,Mouse
A00286 usage in article: APP:WB, SAMPLE:U87MG CELL AND U251 CELL, DILUTION:1:1000

Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
A00286 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000

Anti-endometriosis Mechanism of Jiawei Foshou San Based on Network Pharmacology

Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1%u03B1 and autophagy in human osteosarcoma

Inactivation of lysyl oxidase by %u03B2-aminopropionitrile inhibits hypoxia-induced invasion and migration of cervical cancer cells

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells

Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction

Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation

Association of MMP-2 expression and prognosis in osteosarcoma patients

TGF-?1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21

The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-?1 pathway

Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-?B activation

Ezrin contributes to cervical cancer progression through induction of epithelial-mesenchymal transition

Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-?B Pathway

MMP-1/2 and TIMP-1/2 expression levels, and the levels of collagenous and elastic fibers correlate with disease progression in a hamster model of tongue cancer

Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An?in vitro?investigation

Amniotic Mesenchymal Stem Cells Can Enhance Angiogenic Capacity via MMPs In Vitro and In Vivo

Effect of Ginkgo biloba extract on experimental cardiac remodeling

Efficacy of intravitreal captopril on oxygen-induced retinopathy in mice

Inhibition of CXCR4 activity with AMD3100 decreases invasion of human colorectal cancer cells in vitro

Yang Y, Gao M, Lin Z, Chen L, Jin Y, Zhu G, Wang Y, Jin T. Oncotarget. 2017 Oct 17;8(58):98708-98722. doi: 10.18632/oncotarget.21864. eCollection 2017 Nov 17. DEK promoted EMT and angiogenesis through regulating PI3K/AKT/mTOR pathway in triple-neg...

Li C, Liu J, Zhang Q, Cui K, Ge Q, Wang C, Chen Z. Int J Oncol. 2018 Jun;52(6):1815-1826. doi: 10.3892/ijo.2018.4346. Epub 2018 Mar 29. Upregulation of E-cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder...

Zhang Q, Wang C, Miao S, Li C, Chen Z, Li F. Oncotarget. 2017 Sep 30;8(55):93969-93983. doi: 10.18632/oncotarget.21400. eCollection 2017 Nov 7. Enhancing E-cadherin expression via promoter-targeted miR-373 suppresses bladder cancer cells growth an...

Li P, Chen H, Chen S, Mo X, Li T, Xiao B, Yu R, Guo J. Br J Cancer. 2017 Feb 28;116(5):626-633. doi: 10.1038/bjc.2016.451. Epub 2017 Jan 12 Circular RNA 0000096 affects cell growth and migration in gastric cancer

Wang M, Li M, Yan P, Luo Q, Zhang Y, Du Z. J Ophthalmol. 2017;2017:6067890. doi: 10.1155/2017/6067890. Epub 2017 Feb 9. The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment

Zhang Jt, Sun W, Zhang Wz, Ge Cy, Liu Zy, Zhao Zm, Lu Xs, Fan Yz. Bmc Cancer. 2014 Mar 15;14:193. Doi: 10.1186/1471-2407-14-193. Norcantharidin Inhibits Tumor Growth And Vasculogenic Mimicry Of Human Gallbladder Carcinomas By Suppression Of The Pi...

Zhang Y, Shen Y, Cao B, Yan A, Ji H. Exp Ther Med. 2015 Mar;9(3):905-908. Epub 2014 Dec 19. Elevated Expression Levels Of Androgen Receptors And Matrix Metalloproteinase-2 And -9 In 30 Cases Of Hepatocellular Carcinoma Compared With Adjacent Tissu...

Liu J, Ping W, Zu Y, Sun W. Int J Clin Exp Pathol. 2014 Aug 15;7(9):6040-7. Ecollection 2014. Correlations Of Lysyl Oxidase With Mmp2/Mmp9 Expression And Its Prognostic Value In Non-Small Cell Lung Cancer.

Fu Y, Jiao Y, An N, Liang A. Cytotechnology. 2013 Aug;65(4):533-9. Doi: 10.1007/S10616-012-9518-2. Epub 2012 Dec 15. Pegfp-N1-Mediated Bmk Ct Expression Suppresses The Migration Of Glioma.

Sun Fl, Wang W, Cheng H, Wang Y, Li L, Xue Jl, Wang Xf, Ai Hx, Zhang L, Xu Jd, Wang Xm, Ji Xm. Plos One. 2014 Jun 30;9(6):E101194. Doi: 10.1371/Journal.Pone.0101194. Ecollection 2014. Morroniside Improves Microvascular Functional Integrity Of The ...

Li Jk, Yu L, Shen Y, Zhou Ls, Wang Yc, Zhang Jh. World J Gastroenterol. 2008 Apr 21;14(15):2308-13. Inhibition Of Cxcr4 Activity With Amd3100 Decreases Invasion Of Human Colorectal Cancer Cells In Vitro.

Zhang J, Chen X, Shi G, Xie X, Liu H, Zhang X, Lai Y, Zuo Y, Chen Z, Liu S, Wang H. J Ovarian Res. 2013 Feb 6;6(1):9. Doi: 10.1186/1757-2215-6-9. Establishment Of A New Representative Model Of Human Ovarian Cancer In Mice.

Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.

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15 Customer Q&As for Anti-MMP2 Antibody Picoband™

Question

Will A00286 anti-MMP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

It shows on the product datasheet, A00286 anti-MMP2 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-23

Question

Does anti-MMP2 antibody A00286 work for ELISA with melanoma?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

According to the expression profile of melanoma, MMP2 is highly expressed in melanoma. So, it is likely that anti-MMP2 antibody A00286 will work for ELISA with melanoma.

Boster Scientific Support

Answered: 2020-01-03

Question

I have attached the WB image, lot number and protocol we used for melanoma using anti-MMP2 antibody A00286. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-07

Question

We ordered your anti-MMP2 antibody for ELISA on mucosa of stomach last year. I am using rat, and I plan to use the antibody for WB next. I would like examining mucosa of stomach as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

I viewed the website and datasheets of our anti-MMP2 antibody and it appears that A00286 has been validated on rat in both ELISA and WB. Thus A00286 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-23

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma using anti-MMP2 antibody A00286. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-15

Question

I see that the anti-MMP2 antibody A00286 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-05

Question

We are currently using anti-MMP2 antibody A00286 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

The anti-MMP2 antibody (A00286) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-25

Question

We have seen staining in mouse brain. Any tips? Is anti-MMP2 antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2019-07-02

Answer

According to literature brain does express MMP2. According to Uniprot.org, MMP2 is expressed in mucosa of stomach, teratocarcinoma testis, brain, melanoma, among other tissues. Regarding which tissues have MMP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Melanoma, Pubmed ID: 1480041, 1655733, 8646777
Teratocarcinoma, and Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-07-02

Question

I was wanting to use your anti-MMP2 antibody for ELISA for human melanoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human melanoma identification?

Verified Customer

Verified customer

Asked: 2019-01-16

Answer

As indicated on the product datasheet, A00286 anti-MMP2 antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human melanoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-01-16

Question

We were content with the WB result of your anti-MMP2 antibody. However we have been able to see positive staining in mucosa of stomach isoform 2: cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is MMP2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-10-05

Answer

From what I have seen in literature, mucosa of stomach does express MMP2. Generally MMP2 expresses in isoform 1: secreted, extracellular space,, isoform 2: cytoplasm. mitochondrion. Regarding which tissues have MMP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Melanoma, Pubmed ID: 1480041, 1655733, 8646777
Teratocarcinoma, and Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-10-05

Question

Do you have a BSA free version of anti-MMP2 antibody A00286 available?

Verified Customer

Verified customer

Asked: 2018-07-05

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MMP2 antibody A00286 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-07-05

Question

Is a blocking peptide available for product anti-MMP2 antibody (A00286)?

Verified Customer

Verified customer

Asked: 2018-02-19

Answer

We do provide the blocking peptide for product anti-MMP2 antibody (A00286). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-02-19

Question

Is this A00286 anti-MMP2 antibody reactive to the isotypes of MMP2?

Verified Customer

Verified customer

Asked: 2017-09-15

Answer

The immunogen of A00286 anti-MMP2 antibody is E. coli-derived human MMP2 recombinant protein (Position: K429-C660). Human MMP2 shares 95.7% and 95.3% amino acid (aa) sequence identity with mouse and rat MMP2, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-09-15

Question

We are interested in to test anti-MMP2 antibody A00286 on human melanoma for research purposes, then I may be interested in using anti-MMP2 antibody A00286 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-08-24

Answer

The products we sell, including anti-MMP2 antibody A00286, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-08-24

Question

Can you help my question with product A00286, anti-MMP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Miller

Verified customer

Asked: 2015-11-02

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00286 anti-MMP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-11-02

Size

Total: $315

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A00286
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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