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Product Info Summary
| SKU: | A03449 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-NADP-dependent malic enzyme ME1 Antibody
SKU/Catalog Number
A03449
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NADP-dependent malic enzyme ME1 Antibody (Catalog # A03449). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ME1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-NADP-dependent malic enzyme ME1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03449)
Host
Rabbit
Contents
ME1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ME1 antibody was raised against a 16 amino acid peptide near the amino terminus of human ME1. The immunogen is located within the first 50 amino acids of ME1.
Cross-reactivity
ME1 antibody is human, mouse and rat reactive. ME1 antibody is predicted not to cross-react with ME2.
Reactive Species
A03449 is reactive to ME1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
64.2 kDa
Background of ME1
ME1, also known as NADP-ME, MES or HUMNDME, may play an important role as a housekeeping protein within the cell (1). ME1 is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family (2). It is expressed ubiquitously with highest expression in liver and white adipose tissue. ME1 functions as an NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (3). The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles (3,4). ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03449 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ME1 antibody can be used for detection of ME1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ME1 in 293 cell lysate with ME1 antibody at 1 μg/ml.
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Immunohistochemistry of ME1 in mouse liver tissue with ME1 antibody at 5 μg/mL.
Specific Publications For Anti-NADP-dependent malic enzyme ME1 Antibody (A03449)
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