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Product Info Summary
| SKU: | A00043 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Neurofibromin NF1 Antibody
SKU/Catalog Number
A00043
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Neurofibromin NF1 Antibody catalog # A00043. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neurofibromin NF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00043)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NF1. AA range:1551-1600
Reactive Species
A00043 is reactive to NF1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
319.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00043 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Immunofluorescence analysis of HepG2 cells, using NF1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using Neurofibromin Polyclonal Antibody
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Western Blot analysis of HepG2 cells using Neurofibromin Polyclonal Antibody
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Western blot analysis of lysates from HepG2 cells, using NF1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Neurofibromin NF1 Antibody (A00043)
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3 Customer Q&As for Anti-Neurofibromin NF1 Antibody
Question
I see that the anti-Neurofibromin antibody A00043 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-25
Question
Can you help my question with product A00043, anti-Neurofibromin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00043 anti-Neurofibromin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-14
Question
I was wanting to use your anti-Neurofibromin antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
S. Roberts
Verified customer
Asked: 2014-08-27
Answer
It shows on the product datasheet, A00043 anti-Neurofibromin antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-27
