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Anti-NF-kB p65/RELA Antibody
Boster Bio Anti-NF-kB p65/RELA Antibody catalog # PA1125. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 49 publication(s).
Anti-NF-kB p65/RELA Antibody Product Summary
| SKU: | PA1125 |
|---|---|
| Size: | 100μg/vial |
| Reactivity: | Human, Mouse, Rat |
| Clonality: | Polyclonal |
| Host: | Rabbit |
| Applications: | WB |
| Conjugation: | Choose a conjugate option when adding to cart. Sample size cannot be ordered with conjugation. Conjugated antibody size will be reduced in half due to loss in the conjugation process. |
Overview
| Product Name | Anti-NF-kB p65/RELA Antibody
See more RELA products |
|---|---|
| Catalog# | PA1125 |
| Pack Size | 100μg/vial |
| Storage & Handling | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description | Boster Bio Anti-NF-kB p65/RELA Antibody catalog # PA1125. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. |
| Cite This Product | Anti-NF-kB p65/RELA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1125) |
| Antibodies Validation | Antibodies Validation Information |
| Similar Products From Other Companies |
Anti-NF-kB p65/RELA Antibody may replace the following items: sc 81622. |
Product Specs
| Host | Rabbit |
|---|---|
| Reactive Species | Human, Mouse, Rat |
| Predicted Reactivity | Bovine *This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species. |
| Applications | WB
*Our Boster Guarantee covers the use of this product in the above tested applications. *Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $100. Contact us for more information. **Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human NF-kB p65 (116-131aa AISQRIQTNNNPFQVP), identical to the related rat sequence, and different from the related mouse sequence by one amino acid. |
| Cross Reactivity | No cross reactivity with other proteins |
Gene/Protein Basic Information For RELA (Source: Uniprot.org, NCBI)
| Uniprot Id | Q04206 |
|---|---|
| NCBI Gene Id | 5970 |
| Species Of This Entry | Human |
| Gene Name | RELA |
| Protein Name | Transcription factor p65 |
| Alternative Names | RELA|NF kB p65; NFkB p65; NF-kB p65; p65; rela p65; RelA; MGC131774; nf kb p65; NFkB p65; NF-kB p65; NFKB3; NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)); Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65; p65 flow cytometry; p65 immunohistochemistry; p65; p65RelA; rela p65; RelA; v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight po |
| Molecular Weight | 60219 |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on RELA, check out the RELA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for RELA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Our Boster Quality Guarantee for Anti-NF-kB p65/RELA Antibody covers its use in the following applications.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti-NF-kB p65 antibody, PA1125, Western blotting
All lanes: Anti NF-kB p65 (PA1125) at 0.5ug/ml
Lane 1: Human Colon Cancer Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 65KD
Observed bind size: 65KD
All lanes: Anti NF-kB p65 (PA1125) at 0.5ug/ml
Lane 1: Human Colon Cancer Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 65KD
Observed bind size: 65KD
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 49
| Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154. Species: Mouse PA1125 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA |
PubMed ID 33607154 |
| Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154. Species: Mouse PA1125 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA |
PubMed ID 33607154 |
| Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425. Species: Human PA1125 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:1:2000 |
PubMed ID 32855700 |
| Ma G,Kimatu BM,Yang W,Pei F,Zhao L,Du H,Su A,Hu Q,Xiao H.Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential.J Food Sci.2020 Sep;85(9):2822-2831. doi:10.1111/1750-3841.15375.Epub 2020 Aug 14 Species: Mouse PA1125 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA |
PubMed ID 32794226 |
| Wang Y,Yang Z,Yang L,Zou Q,Zhao S,Hu N,Chen D,Cui R,Ma H.Liuweidihuang Pill Alleviates Inflammation of the Testis via AMPK/SIRT1/NF-κB Pathway in Aging Rats.Evid Based Complement Alternat Med.2020 May 24;2020:2792738.doi:10.1155/2020/2792738.PMID:32565851 Species: Rat PA1125 usage in article: APP:WB, SAMPLE:TESTICULAR TISSUE, DILUTION:NA |
PubMed ID 32565851 |
| Qi MY,Wang XT,Xu HL,Yang ZL,Cheng Y,Zhou B.Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats.Food Funct.2020 Apr 1;11(4):3706-3718.doi:10.1039/c9fo02398d.Epub 2020 Apr 20. PMID:32307498. Species: Rat PA1125 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000 |
PubMed ID 32307498 |
| Wei JL,Wu CJ,Chen JJ,Shang FT,Guo SG,Zhang XC,Liu H.LncRNA NEAT1 promotes the progression of sepsis-induced myocardial cell injury by sponging miR-144-3p.Eur Rev Med Pharmacol Sci.2020 Jan;24(2):851-861.doi:10.26355/eurrev_202001_20069.PMID:32016991. Species: Mouse PA1125 usage in article: APP:WB, SAMPLE:HL-1 CELLS, DILUTION:1:1000 |
PubMed ID 32016991 |
| Nuclear factor-kappaB activation on the reactive oxygen species in acute necrotizing pancreatitic rats | PubMed ID 16015706 |
| N-acetylcysteine reduces oxidative stress, nuclear factor-?B activity and cardiomyocyte apoptosis in heart failure | PubMed ID 24889421 |
| Src Promotes Metastasis of Human Non-Small Cell Lung Cancer Cells through Fn14-Mediated NF-?B Signaling | PubMed ID 29500337 |
| Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats | PubMed ID 29484403 |
| Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction | PubMed ID 29390038 |
| Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro | PubMed ID 29285085 |
| Effect of stromal cell-derived factor-1 on myocardial apoptosis and cardiac function recovery in rats with acute myocardial infarction | PubMed ID 27882150 |
| Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats | PubMed ID 27641696 |
| Zisheng Shenqi decoction ameliorates monosodium urate crystal-induced gouty arthritis in rats through anti-inflammatory and anti-oxidative effects | PubMed ID 27432278 |
| Simvastatin ameliorates renal lipidosis through the suppression of renal CXCL16 expression in mice with adriamycin-induced nephropathy. | PubMed ID 26884839 |
| CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment | PubMed ID 26526388 |
| Down-regulation of HMGB1 expression by shRNA constructs inhibits the bioactivity of urothelial carcinoma cell lines via the NF-?B pathway | PubMed ID 26239046 |
| microRNA-146a inhibits cancer metastasis by downregulating VEGF through dual pathways in hepatocellular carcinoma | PubMed ID 25608619 |
| Molecular responses of radiation-induced liver damage in rats | PubMed ID 25483171 |
| MiR199b Suppresses Expression of Hypoxia-Inducible Factor 1? (HIF-1?) in Prostate Cancer Cells | PubMed ID 23594994 |
| Ozone preconditioning and exposure to ketamine attenuates hepatic inflammation in septic rats | PubMed ID 23185204 |
| Histone deacetylase inhibitor, butyrate, attenuates lipopolysaccharide-induced acute lung injury in mice | PubMed ID 20302656 |
| Oligomannurarate sulfate blocks tumor growth by inhibiting NF-?B activation | PubMed ID 20154712 |
| PD-L1 is upregulated by EBV-driven LMP1 through NF-?B pathway and correlates with poor prognosis in natural killer/T-cell lymphoma | PubMed ID 27737703 |
| Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice | PubMed ID 27413418 |
| Gypenoside Protects Cardiomyocytes against Ischemia-Reperfusion Injury via the Inhibition of Mitogen-Activated Protein Kinase Mediated Nuclear Factor Kappa B Pathway In Vitro and In Vivo | PubMed ID 27313532 |
| Silencing of carboxypeptidase E inhibits cell proliferation, tumorigenicity, and metastasis of osteosarcoma cells | PubMed ID 27274275 |
| Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway | PubMed ID 27164829 |
| Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-?B | PubMed ID 27150105 |
| Effects of resveratrol and genistein on nuclear factor??B, tumor necrosis factor?? and matrix metalloproteinase?9 in patients with chronic obstructive pulmonary disease | PubMed ID 27035424 |
| Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-?B activation | PubMed ID 27013894 |
| Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-?B Signaling Pathway | PubMed ID 26904148 |
| BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation | PubMed ID 26880952 |
| Fenofibrate, a PPAR? agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB | PubMed ID 26617775 |
| Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-?B and TGF-?1/Smad-2/-3 pathways | PubMed ID 26577463 |
| Protective Effects of Hydrogen-Rich Saline on Rats with Smoke Inhalation Injury | PubMed ID 26090070 |
| Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury | PubMed ID 25657665 |
| Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-?B in Acute Transection Spinal Cord Injury in Rats | PubMed ID 25535513 |
| Dual effects of ouabain on the regulation of proliferation and apoptosis in human umbilical vein endothelial cells: involvement of Na+-K+-ATPase ?-subunits and NF-?B | PubMed ID 24995076 |
| Effect of Aspirin on the Expression of Hepatocyte NF-?B and Serum TNF-? in Streptozotocin-Induced Type 2 Diabetic Rats | PubMed ID 21655062 |
| Sun, G., Yang, W., Zhang, Y., & Zhao, M. (2017). Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats. Experimental Animals. Advance online publication. doi: 10.1538/expanim.16-0102 | PubMed ID 28637971 |
| Zhang Qq, Ding Y, Lei Y, Qi Cl, He Xd, Lan T, Li Jc, Gong P, Yang X, Geng Jg, Wang Lj. Int J Biol Sci. 2014 Mar 21;10(4):404-14. Doi: 10.7150/Ijbs.7723. Ecollection 2014. Andrographolide Suppress Tumor Growth By Inhibiting Tlr4/Nf-??b Signaling Ac... | PubMed ID 24719558 |
| Wang J, Du Jr, Wang Y, Kuang X, Wang Cy. Acta Pharmacol Sin. 2010 Jul;31(7):791-7. Doi: 10.1038/Aps.2010.71. Epub 2010 Jun 28. Z-Ligustilide Attenuates Lipopolysaccharide-Induced Proinflammatory Response Via Inhibiting Nf-Kappab Pathway In Primary... | PubMed ID 20581853 |
| Liu Mw, Su Mx, Zhang W, Wang Yh, Qin Lf, Liu X, Tian Ml, Qian Cy. Mol Med Rep. 2015 May;11(5):3308-16. Doi: 10.3892/Mmr.2015.3146. Epub 2015 Jan 7. Effect Of Melilotus Suaveolens Extract On Pulmonary Microvascular Permeability By Downregulating Va... | PubMed ID 25571852 |
| Yuan W, Yang D, Sun X, Liu W, Wang L, Li X, Man X, Fu Q. Int J Clin Exp Pathol. 2014 Aug 15;7(9):5772-81. Ecollection 2014. Effects Of Hydroxysafflor Yellow A On Proliferation And Collagen Synthesis Of Rat Vascular Adventitial Fibroblasts Induced ... | PubMed ID 25337218 |
| Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury. | PubMed ID 26064215 |
| Fan Z, Yao J, Li Y, Hu X, Shao H, Tian X. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3451-9. Ecollection 2015. Anti-Inflammatory And Antioxidant Effects Of Curcumin On Acute Lung Injury In A Rodent Model Of Intestinal Ischemia Reperfusion By Inhibitin... | PubMed ID 26097529 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to PA1125 Anti-NF-kB p65/RELA Antibody
5 Related Questions
Question
Will anti-NF-kB p65/RELA antibody PA1125 work on horse WB with tibial artery?
K. Roberts
Asked: 2019-10-30
Answer
Our lab technicians have not validated anti-NF-kB p65/RELA antibody PA1125 on horse. You can run a BLAST between horse and the immunogen sequence of anti-NF-kB p65/RELA antibody PA1125 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse tibial artery in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone using anti-NF-kB p65/RELA antibody PA1125. Let me know if you need anything else.
N. Jones
Asked: 2017-10-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-12
Question
Can you help my question with product PA1125, anti-NF-kB p65/RELA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Collins
Asked: 2017-08-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1125 anti-NF-kB p65/RELA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-04
Question
Would PA1125 anti-NF-kB p65/RELA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Asked: 2017-06-12
Answer
It shows on the product datasheet, PA1125 anti-NF-kB p65/RELA antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-12
Question
Is there a BSA free version of anti-NF-kB p65/RELA antibody PA1125 available?
B. Thomas
Asked: 2015-08-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NF-kB p65/RELA antibody PA1125 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-08-21
Order Product (PA1125)
Shipping Information
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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Secondary Antibodies
For a full list of fitting secondary antibodies, conjugated with all common conjugates for WB and IHC applications, click the link below.
All secondary antibodiesBlocking Peptide & Immunogen Protein
To order the blocking peptide or the immungen protein ($99 / 1mg) for Anti-NF-kB p65/RELA Antibody PA1125, contact [email protected] for a quote. Or use the live chat function.
Control Lysates
To order the RELA over expression cell lysate, contact [email protected] for more information and a quote. For wild type control lysates we used in QC for this antibody, see below link.
See all cell lysatesCustom Antibodies
Did you know Boster offer custom antibody production service? You can have your antibodies custom made for as low as $600 if your research fit one of these 3 criteria:
- COVID-19 related;
- rare species;
- replacing discountinued antibodies.
Contact Information
| [email protected] | |
| (888) 466-3604 |
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PA1125
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00
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