Anti-NF-kB p65/RELA Antibody

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Datasheet

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SKU PA1125
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-NF-kB p65/RELA Antibody
SKU/Catalog Number PA1125
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Transcription factor p65(RELA) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-NF-kB p65/RELA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1125)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human NF-kB p65(116-131aa AISQRIQTNNNPFQVP), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/pa1125 1 WB anti nf kb p65 antibody.jpg

Anti-NF-kB p65 antibody, PA1125, Western blotting
All lanes: Anti NF-kB p65 (PA1125) at 0.5ug/ml
Lane 1: Human Colon Cancer Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 65KD
Observed bind size: 65KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q04206
Gene Name RELA
Protein Name Transcription factor p65
Alternative Names Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;RELA;NFKB3;
Subcellular Localization Nucleus. Cytoplasm. Colocalized with DDX1 in the nucleus upon TNF-alpha induction (By similarity). Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation. .
Molecular Weight 60219 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). .
Research Areas Apoptosis, Apoptotic Markers, Cancer, Cell Biology, Cell Death, Domain Families, Epigenetics And Nuclear Signaling, Intracellular, Metabolic Signaling Pathways, Metabolism, Mitochondrial Metabolism, Nfkb Pathway, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling, Nuclear Signaling Pathways, Nucleotide Metabolism, Pathways And Processes, Signal Transduction, Signaling Pathway, Transcription, Zinc Finger

*You can search these to find other products in these research areas.
Background The p65(RELA) heterodimer is the most abundant form of NFKB. This gene is located on 11q13, which consists of 10 exons and spans about 8.1 kb of DNA. In rat sciatic nerves, the expression of the activated p65 subunit of NFKB was high in the nuclei of premyelinating Schwann cells and then progressively declined until it was nearly absent in adults. The transcriptional activity of NF-kappa-B is stimulated upon phosphorylation of its p65 subunit on serine-276 by protein kinase A(PKA). The transcriptional coactivator CBP/p300 associates with NF-kappa-B p65 through 2 sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, and a second domain that only interacts with p65 phosphorylated on serine-276.

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Order Product (PA1125)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1054 $240
100ug+Free Biotin Secondary BA1003 $240

USD $240

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Troubleshooting

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Publications

Nuclear factor-kappaB activation on the reactive oxygen species in acute necrotizing pancreatitic rats
PubMed ID16015706
N-acetylcysteine reduces oxidative stress, nuclear factor-?B activity and cardiomyocyte apoptosis in heart failure
PubMed ID24889421
Src Promotes Metastasis of Human Non-Small Cell Lung Cancer Cells through Fn14-Mediated NF-?B Signaling
PubMed ID29500337
Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats
PubMed ID29484403
Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction
PubMed ID29390038
Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro
PubMed ID29285085
Effect of stromal cell-derived factor-1 on myocardial apoptosis and cardiac function recovery in rats with acute myocardial infarction
PubMed ID27882150
Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats
PubMed ID27641696
Zisheng Shenqi decoction ameliorates monosodium urate crystal-induced gouty arthritis in rats through anti-inflammatory and anti-oxidative effects
PubMed ID27432278
Simvastatin ameliorates renal lipidosis through the suppression of renal CXCL16 expression in mice with adriamycin-induced nephropathy.
PubMed ID26884839
CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment
PubMed ID26526388
Down-regulation of HMGB1 expression by shRNA constructs inhibits the bioactivity of urothelial carcinoma cell lines via the NF-?B pathway
PubMed ID26239046
microRNA-146a inhibits cancer metastasis by downregulating VEGF through dual pathways in hepatocellular carcinoma
PubMed ID25608619
Molecular responses of radiation-induced liver damage in rats
PubMed ID25483171
MiR199b Suppresses Expression of Hypoxia-Inducible Factor 1? (HIF-1?) in Prostate Cancer Cells
PubMed ID23594994
Ozone preconditioning and exposure to ketamine attenuates hepatic inflammation in septic rats
PubMed ID23185204
Histone deacetylase inhibitor, butyrate, attenuates lipopolysaccharide-induced acute lung injury in mice
PubMed ID20302656
Oligomannurarate sulfate blocks tumor growth by inhibiting NF-?B activation
PubMed ID20154712
PD-L1 is upregulated by EBV-driven LMP1 through NF-?B pathway and correlates with poor prognosis in natural killer/T-cell lymphoma
PubMed ID27737703
Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice
PubMed ID27413418
Gypenoside Protects Cardiomyocytes against Ischemia-Reperfusion Injury via the Inhibition of Mitogen-Activated Protein Kinase Mediated Nuclear Factor Kappa B Pathway In Vitro and In Vivo
PubMed ID27313532
Silencing of carboxypeptidase E inhibits cell proliferation, tumorigenicity, and metastasis of osteosarcoma cells
PubMed ID27274275
Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway
PubMed ID27164829
Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-?B
PubMed ID27150105
Effects of resveratrol and genistein on nuclear factor??B, tumor necrosis factor?? and matrix metalloproteinase?9 in patients with chronic obstructive pulmonary disease
PubMed ID27035424
Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-?B activation
PubMed ID27013894
Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-?B Signaling Pathway
PubMed ID26904148
BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation
PubMed ID26880952
Fenofibrate, a PPAR? agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB
PubMed ID26617775
Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-?B and TGF-?1/Smad-2/-3 pathways
PubMed ID26577463
Protective Effects of Hydrogen-Rich Saline on Rats with Smoke Inhalation Injury
PubMed ID26090070
Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury
PubMed ID25657665
Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-?B in Acute Transection Spinal Cord Injury in Rats
PubMed ID25535513
Dual effects of ouabain on the regulation of proliferation and apoptosis in human umbilical vein endothelial cells: involvement of Na+-K+-ATPase ?-subunits and NF-?B
PubMed ID24995076
Effect of Aspirin on the Expression of Hepatocyte NF-?B and Serum TNF-? in Streptozotocin-Induced Type 2 Diabetic Rats
PubMed ID21655062
Sun, G., Yang, W., Zhang, Y., & Zhao, M. (2017). Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats. Experimental Animals. Advance online publication. doi: 10.1538/expanim.16-0102
PubMed ID28637971
Zhang Qq, Ding Y, Lei Y, Qi Cl, He Xd, Lan T, Li Jc, Gong P, Yang X, Geng Jg, Wang Lj. Int J Biol Sci. 2014 Mar 21;10(4):404-14. Doi: 10.7150/Ijbs.7723. Ecollection 2014. Andrographolide Suppress Tumor Growth By Inhibiting Tlr4/Nf-??b Signaling Ac...
PubMed ID24719558
Wang J, Du Jr, Wang Y, Kuang X, Wang Cy. Acta Pharmacol Sin. 2010 Jul;31(7):791-7. Doi: 10.1038/Aps.2010.71. Epub 2010 Jun 28. Z-Ligustilide Attenuates Lipopolysaccharide-Induced Proinflammatory Response Via Inhibiting Nf-Kappab Pathway In Primary...
PubMed ID20581853
Liu Mw, Su Mx, Zhang W, Wang Yh, Qin Lf, Liu X, Tian Ml, Qian Cy. Mol Med Rep. 2015 May;11(5):3308-16. Doi: 10.3892/Mmr.2015.3146. Epub 2015 Jan 7. Effect Of Melilotus Suaveolens Extract On Pulmonary Microvascular Permeability By Downregulating Va...
PubMed ID25571852
Yuan W, Yang D, Sun X, Liu W, Wang L, Li X, Man X, Fu Q. Int J Clin Exp Pathol. 2014 Aug 15;7(9):5772-81. Ecollection 2014. Effects Of Hydroxysafflor Yellow A On Proliferation And Collagen Synthesis Of Rat Vascular Adventitial Fibroblasts Induced ...
PubMed ID25337218
Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.
PubMed ID26064215
Fan Z, Yao J, Li Y, Hu X, Shao H, Tian X. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3451-9. Ecollection 2015. Anti-Inflammatory And Antioxidant Effects Of Curcumin On Acute Lung Injury In A Rodent Model Of Intestinal Ischemia Reperfusion By Inhibitin...
PubMed ID26097529

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to p65 antibody, rela antibody