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- Table of Contents
RELA antibody (P00284) performs well in Flow Cytometry, IP, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Western blot analysis of Phospho-NF-κB p65 (S529) expression in HeLa cell lysates treated with Calyculin A and TNF-alpha.
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Anti-Phospho-NF-Kappa B p65 (S529) RELA Rabbit Monoclonal Antibody
RELA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IP, WB |
Tested Applications
RELA antibody was positively detected by WB in:
HeLa cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-1:2000
FC 1:50
IP 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Phospho-NF-Kappa B p65 (S529) RELA Rabbit Monoclonal Antibody
View all RELA Antibodies
View all RELA Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AbN84
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NF-Kappa B p65
Observed Molecular Weight
65 kDa
Calculated Molecular Weight
60.2 kDa
Cite This Product
Anti-Phospho-NF-Kappa B p65 (S529) RELA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00284)
Protocols
Western Blot protocol for RELA antibody
Publications
Application:
Species:
IRE1α/TRAF2/NF-κB pathway promotes apoptosis via regulating inflammatory cytokines and aggravates brain injury after SAH
Published: 2025-03-13
Journal: Journal of Stroke & Cerebrovascular Diseases
Application: WB
Impact Factor: 1.80
Species: Rat
PMID: 40089218
Leonurine Preconditioning Attenuates Ischemic Acute Kidney Injury in Rats by Promoting Nrf2 Nuclear Translocation and Suppressing TLR4/NF-κB Pathway
Published: 2022-01-01
Journal: CHEMICAL & PHARMACEUTICAL BULLETIN
Application: WB
Impact Factor: 1.90
Species: Rat
PMID: 34980736
LncRNA NEAT1 promotes apoptosis and inflammation in LPS‑induced sepsis models by targeting miR‑590‑3p
Published: 2020-07-29
Journal: Experimental and Therapeutic Medicine
Application: WB
Impact Factor: 1.79
Species: Human
PMID: 32855700
Downregulation of ENDOCAN in myeloid leukemia cells inhibits proliferation and promotes apoptosis by suppressing nuclear factor‑κB activity
Published: 2019-02-19
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.85
Species: Human
PMID: 30816462
Leucine aminopeptidase 3 promotes migration and invasion of breast cancer cells through upregulation of fascin and matrix metalloproteinases-2/9 expression
Published: 2018-11-11
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 2.96
Species: Human
PMID: 30417585
Review
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Product Promise
We promise Anti-Phospho-NF-Kappa B p65 (S529) RELA Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P00284
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026