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NOS3 antibody (A01604-2) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 36 Publications

A01604 2 NOS3 primary antibodies FC testing 5

Flow Cytometry analysis of HepG2 cells using anti-NOS3 antibody (A01604-2).
Overlay histogram showing HepG2 cells stained with A01604-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOS3 Antibody (A01604-2,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-eNOS/NOS3 Antibody Picoband®

NOS3 antibody

36 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, Flow Cytometry, IHC, WB

Tested Applications

NOS3 antibody was positively detected by WB in:

human placenta tissue, human K562 whole cell, human HepG2 whole cell, human THP-1 whole cell, rat kidney tissue, mouse kidne tissue, mouse small intestine tissue,

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

NOS3 antibody was positively detected by IHC in:

human lung cancer tissue, rat spleen tissue, mouse spleen tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

NOS3 antibody was positively detected by FC in:

HepG2 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Bovine

Predicted

Dog

Predicted

Rabbit

Predicted

Sheep

Predicted

Product Information

Product Name

Anti-eNOS/NOS3 Antibody Picoband®
View all NOS3 Antibodies

Form

Lyophilized

Uniprot

P29474

Gene ID

4846

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human eNOS/NOS3 recombinant protein (Position: Q1052-P1203).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

133 kDa

Calculated Molecular Weight

133.3 kDa

Background of NOS3

NOS3 (Nitric Oxide Synthase 3), also called ENOS, a nitric oxide synthase that generates NO in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction and platelet aggregation. The NOS3 gene is mapped on 7q36.1. Variations in this gene are associated with susceptibility tocoronary spasm. Fulton et al. concluded the eNOS is an AKT substrate linking signal transduction by AKT to the release of the gaseous second messenger nitric oxide. AKT mediates the activation of eNOS, leading to increased nitric oxide production. Inhibition of the PI3K AKT pathway or mutation of the AKT site on eNOS protein at serine-1177 attenuated the serine phosphorylation and prevented the activation of eNOS. RT-PCR analysis showed that expression of NOS3 in human umbilical vein endothelial cells (HUVECs) and human aortic vascular smooth muscle cells (HAOVSMCs) was inversely proportional to that of NOS3AS.

Cite This Product

Anti-eNOS/NOS3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01604-2)

Protocols

Western Blot protocol for NOS3 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for NOS3 antibody

Review Protocol

Flow Cytometry (FC) protocol for NOS3 antibody

Review Protocol

Publications

Evaluation of 17β-estradiol treatment in rat lungs after circulatory death using in situ perfusion

Published: 2026-08-21

Journal: TRANSPLANT IMMUNOLOGY

Application: IHC

Impact Factor: 1.60

Species: Rat

PMID: 42628585

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Anti-eNOS/NOS3 Antibody Picoband®

NOS3 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Evaluation of 17β-estradiol treatment in rat lungs after circulatory death using in situ perfusion

Nephrotoxicity of Evodiamine in Mice: Mechanistic Insights from Integrated Network Toxicology and Transcriptomic Profiling

Low-dose urapidil mitigates renal ischemia-reperfusion injury through matrix metalloproteinase-9 inhibition and anti-inflammatory effects

Hormonal Treatment During Ex Vivo Lung Perfusion Ameliorates Brain Death Induced Inflammation

Deciphering the molecular mechanisms underlying of Xin Xue Granule on LPS-induced pyrexia rats: A multi-omics analysis

Sex differences in kidney and lung status in an animal model of brain death

NOSTRIN is an emerging positive regulator of decompensated cirrhotic patients with portal hypertension

Comparison of acute kidney injury following brain death between male and female rats

Placental expression of striatin & endothelial nitric oxide synthase in women with & without pre-eclampsia

Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis

The inhibitory effects of Dulaglutide on cellular senescence against high glucose in human retinal endothelial cells

Increased secretion of VEGF-C from SiO2-induced pulmonary macrophages promotes lymphangiogenesis through the Src/eNOS pathway in silicosis

Buxuhuayu decoction accelerates angiogenesis by activating the PI3K-Akt-eNOS signalling pathway in a streptozotocin-induced diabetic ulcer rat model

Atorvastatin improves left ventricular remodeling and cardiac function in rats with congestive heart failure by inhibiting RhoA/Rho kinase‑mediated endothelial nitric oxide synthase

Role of NADPH oxidase pathway in renal protection induced by procyanidin B2: In L-NAME induced rat hypertension model

Pioglitazone, a Peroxisome Proliferator-Activated Receptor γ Agonist, Ameliorates Chronic Kidney Disease by Enhancing Antioxidative Capacity and Attenuating Angiogenesis in the Kidney of a 5/6 Nephrectomized Rat Model

Fucoidan from Undaria pinnatifida prevents vascular dysfunction through PI3K/Akt/eNOS-dependent mechanisms in the L-NAME-induced hypertensive rat model

Autologous Bone Marrow Mononuclear Cell Transplantation Delays Progression of Carotid Atherosclerosis in Rabbits

Effects of thyroid hormones on the antioxidative status in the uterus of young adult rats

Nitric oxide and thyroid hormone receptor alpha 1 contribute to ovarian follicular development in immature hyper- and hypo-thyroid rats

Sodium ferulate lowers portal pressure in rats with secondary biliary cirrhosis through the RhoA/Rho-kinase signaling pathway: A preliminary study

Reproductive toxicity in acrylamide-treated female mice

Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and soluble guanylyl cyclase α and β subunits in postnatal porcine uteri

Sesamin ameliorates arterial dysfunction in spontaneously hypertensive rats via downregulation of NADPH oxidase subunits and upregulation of eNOS expression

Intermittent maternal hypoxia has an influence on regional expression of endothelial nitric oxide synthase in fetal arteries of rabbits

Endothelial NO synthase and reactive oxygen species mediated effect of simvastatin on vessel structure and function: Pleiotropic and dose-dependent effect on tumor vascular stabilization

Puerarin decreases hypoxia inducible factor-1 alpha in the hippocampus of vascular dementia rats

Sesamin exerts renoprotective effects by enhancing NO bioactivity in renovascular hypertensive rats fed with high-fat-sucrose diet

2,3,4′,5-Tetrahydroxystilbene-2-O-β-d-glucoside Inhibits Proliferation of Vascular Smooth Muscle Cells: Involvement of NO/cGMP/PKG Pathway

Propofol improves cardiac functional recovery after ischemia-reperfusion by upregulating nitric oxide synthase activity in the isolated rat hearts

Sesamin improves endothelial dysfunction in renovascular hypertensive rats fed with a high-fat, high-sucrose diet

Effects of Quercetin on Gene and Protein Expression of NOX and NOS after Myocardial Ischemia and Reperfusion in Rabbit

Effects of Calcitonin Gene-Related Peptide on the Expression and Activity of Nitric Oxide Synthase During Mandibular Bone Healing in Rabbits: An Experimental Study

Protective effects of 2,3,5,4′-tetrahydroxystilbene-2-O-beta-d-glucoside, an active component of Polygonum multiflorum Thunb, on experimental colitis in mice

Inhibition by brimonidine of forskolin-induced nitric oxide synthase expression in human ciliary bodies in vitro

Research on the Age-Related Changes in the Nitric Oxide Pathway in the Arteries of Rats and the Intervention Effect of Dehydroepiandrosterone

Review

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