Anti-eNOS/NOS3 Antibody Picoband™

eNOS antibody

Boster Bio Anti-eNOS/NOS3 Antibody Picoband™ catalog # A01604-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 23 publication(s).

Product Info Summary

SKU: A01604-2
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC, WB

Product Name

Anti-eNOS/NOS3 Antibody Picoband™

View all eNOS Antibodies

SKU/Catalog Number

A01604-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-eNOS/NOS3 Antibody Picoband™ catalog # A01604-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-eNOS/NOS3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01604-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human eNOS/NOS3 recombinant protein (Position: Q1052-P1203).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A01604-2 is reactive to NOS3 in Human, Mouse, Rat

Applications

A01604-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Background of eNOS

NOS3 (Nitric Oxide Synthase 3), also called ENOS, a nitric oxide synthase that generates NO in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction and platelet aggregation. The NOS3 gene is mapped on 7q36.1. Variations in this gene are associated with susceptibility to?coronary spasm. Fulton et al. concluded the eNOS is an AKT substrate linking signal transduction by AKT to the release of the gaseous second messenger nitric oxide. AKT mediates the activation of eNOS, leading to increased nitric oxide production. Inhibition of the PI3K AKT pathway or mutation of the AKT site on eNOS protein at serine-1177 attenuated the serine phosphorylation and prevented the activation of eNOS. RT-PCR analysis showed that expression of NOS3 in human umbilical vein endothelial cells (HUVECs) and human aortic vascular smooth muscle cells (HAOVSMCs) was inversely proportional to that of NOS3AS.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Huma

Validation Images & Assay Conditions

Gene/Protein Information For NOS3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P29474

Gene ID

4846

Gene Name

NOS3

Full Name

Nitric oxide synthase, endothelial

Weight

133.275kDa

Superfamily

NOS family

Alternative Names

Constitutive NOS; EC 1.14.13.39; ECNOS; Endothelial NOS; endothelial; eNOS; eNOScNOS; nitric oxide synthase 3 (endothelial cell); NOS type III; NOS3; NOSIII NOS3 ECNOS, eNOS nitric oxide synthase 3 nitric oxide synthase, endothelial|EC-NOS|NOS type III|NOSIII|cNOS|constitutive NOS|endothelial NOS|nitric oxide synthase 3 (endothelial cell)|nitric oxide synthase 3 transcript variant eNOS-delta20|nitric oxide synthase 3 transcript variant eNOS-delta20-21|nitric oxide synthase 3 transcript variant eNOS-delta21

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NOS3, check out the NOS3 Infographic

NOS3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NOS3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A01604-2 has been cited in 23 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Inhibition by brimonidine of forskolin-induced nitric oxide synthase expression in human ciliary bodies in vitro

Propofol improves cardiac functional recovery after ischemia-reperfusion by upregulating nitric oxide synthase activity in the isolated rat hearts

Research on the Age-Related Changes in the Nitric Oxide Pathway in the Arteries of Rats and the Intervention Effect of Dehydroepiandrosterone

Effects of Quercetin on Gene and Protein Expression of NOX and NOS after Myocardial Ischemia and Reperfusion in Rabbit

Fucoidan from Undaria pinnatifida prevents vascular dysfunction through PI3K/Akt/eNOS-dependent mechanisms in the L-NAME-induced hypertensive rat model

Effects of Calcitonin Gene-Related Peptide on the Expression and Activity of Nitric Oxide Synthase During Mandibular Bone Healing in Rabbits: An Experimental Study

Sesamin improves endothelial dysfunction in renovascular hypertensive rats fed with a high-fat, high-sucrose diet

Protective effects of 2,3,5,4′-tetrahydroxystilbene-2-O-beta-d-glucoside, an active component of Polygonum multiflorum Thunb, on experimental colitis in mice

Increased secretion of VEGF-C from SiO2-induced pulmonary macrophages promotes lymphangiogenesis through the Src/eNOS pathway in silicosis

Autologous Bone Marrow Mononuclear Cell Transplantation Delays Progression of Carotid Atherosclerosis in Rabbits

2,3,4′,5‐Tetrahydroxystilbene‐2‐O‐β‐d‐glucoside Inhibits Proliferation of Vascular Smooth Muscle Cells: Involvement of NO/cGMP/PKG Pathway

Zhang J,Cui J,Li X,Hao X,Guo L,Wang H,Liu H.Increased secretion of VEGF-C from SiO2-induced pulmonary macrophages promotes lymphangiogenesis through the Src/eNOS pathway in silicosis.Ecotoxicol Environ Saf.2021 Apr 29;218:112257.doi:10.1016/j.ecoenv.2021.112257.Epub ahead of print.PMID:33933809.
Species: Human
A01604-2 usage in article: APP:WB, SAMPLE:LECS, DILUTION:NA

Qu K,Cha H, Ru Y,Que H,Xing M.Buxuhuayu Decoction Accelerates Angiogenesis by Activating the PI3K-Akt-eNOS Signalling Pathway in a Streptozotocin-Induced Diabetic Ulcer Rat Model.J Ethnopharmacol.2021 Feb 10:113824.doi:10.1016/j.jep.2021.113824.Epub ahead
Species: Rat
A01604-2 usage in article: APP:WB, SAMPLE:WOUND TISSUE, DILUTION:1:800

The Puerarin improves renal function in STZ-induced diabetic rats by attenuating eNOS expression

Ligustrazine modulates renal cysteine biosynthesis in rats exposed to cadmium

Expression of endothelial nitric oxide synthase and vascular endothelial growth factor in association with neovascularization in human primary astrocytoma

Chen Y, Zhang S, Peng G, Yu J, Liu T, Meng R, Li Z, Zhao Y, Wu G. Int J Oncol. 2013 Apr;42(4):1325-36. Doi: 10.3892/Ijo.2013.1833. Epub 2013 Feb 19. Endothelial No Synthase And Reactive Oxygen Species Mediated Effect Of Simvastatin On Vessel Struc...

Zhang Jx, Yang Jr, Chen Gx, Tang Lj, Li Wx, Yang H, Kong X. Acta Pharmacol Sin. 2013 Jul;34(7):912-20. Doi: 10.1038/Aps.2013.1. Epub 2013 Apr 29. Sesamin Ameliorates Arterial Dysfunction In Spontaneously Hypertensive Rats Via Downregulation Of Nad...

Kong L, Wei Q, Fedail Js, Shi F, Nagaoka K, Watanabe G. J Reprod Dev. 2015;61(3):219-27. Doi: 10.1262/Jrd.2014-129. Epub 2015 Mar 20. Effects Of Thyroid Hormones On The Antioxidative Status In The Uterus Of Young Adult Rats.

Zhang W, Wei Qw, Wang Zc, Ding W, Wang W, Shi Fx. J Zhejiang Univ Sci B. 2011 Jan;12(1):55-64. Doi: 10.1631/Jzus.B1000174. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And The Related Nitric Oxide/Cyclic Gmp Si...

Wu R, Yin J, Yao K, Haefliger I. Mol Vis. 2007 Apr 2;13:493-6. Inhibition By Brimonidine Of Forskolin-Induced Nitric Oxide Synthase Expression In Human Ciliary Bodies In Vitro.

Wu H, Wang H, Zhang B, Zhang G, Zhang R, Zhang L. Neural Regen Res. 2012 Feb 25;7(6):421-5. Doi: 10.3969/J.Issn.1673-5374.2012.06.003. Puerarin Decreases Hypoxia Inducible Factor-1 Alpha In The Hippocampus Of Vascular Dementia Rats.

Wei L, Yang J, Wang M, Xu Sn, Liang Hm, Zhou Q. Int J Mol Med. 2014 Nov;34(5):1257-67. Doi: 10.3892/Ijmm.2014.1905. Epub 2014 Aug 19. Sodium Ferulate Lowers Portal Pressure In Rats With Secondary Biliary Cirrhosis Through The Rhoa/Rho-Kinase Signa...

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16 Customer Q&As for Anti-eNOS/NOS3 Antibody Picoband™

Question

Does anti-eNOS/NOS3 antibody A01604-2 work for ELISA with umbilical vein?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

According to the expression profile of umbilical vein, NOS3 is highly expressed in umbilical vein. So, it is likely that anti-eNOS/NOS3 antibody A01604-2 will work for ELISA with umbilical vein.

Boster Scientific Support

Answered: 2020-03-17

Question

Do you have a BSA free version of anti-eNOS/NOS3 antibody A01604-2 available?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-eNOS/NOS3 antibody A01604-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-19

Question

Is a blocking peptide available for product anti-eNOS/NOS3 antibody (A01604-2)?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

We do provide the blocking peptide for product anti-eNOS/NOS3 antibody (A01604-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-23

Question

We are currently using anti-eNOS/NOS3 antibody A01604-2 for mouse tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

The anti-eNOS/NOS3 antibody (A01604-2) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-11

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical vein using anti-eNOS/NOS3 antibody A01604-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-04-16

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-04-16

Question

I am interested in using your anti-eNOS/NOS3 antibody for positive regulation of blood vessel endothelial cell migration studies. Has this antibody been tested with western blotting on small intestine tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-04-03

Answer

We appreciate your inquiry. This A01604-2 anti-eNOS/NOS3 antibody is tested on human placenta tissue, k562 whole cell lysates, hepg2 whole cell lysates, rat spleen tissue, kidney tissue, mouse kidne tissue lysates, small intestine tissue, u87 cells. It is guaranteed to work for ELISA, Flow Cytometry, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-04-03

Question

Our team were happy with the WB result of your anti-eNOS/NOS3 antibody. However we have observed positive staining in spleen caveola. cytoplasm, using this antibody. Is that expected? Could you tell me where is NOS3 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-10-30

Answer

From literature, spleen does express NOS3. Generally NOS3 expresses in cell membrane. membrane, caveola. cytoplasm,. Regarding which tissues have NOS3 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Hippocampus, and Trachea, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7509596, 7514568, 7515611, 15489334
Platelet, Pubmed ID: 7475956
Spleen, Pubmed ID: 12853948

Boster Scientific Support

Answered: 2018-10-30

Question

I have attached the WB image, lot number and protocol we used for umbilical vein using anti-eNOS/NOS3 antibody A01604-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-10-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-10-03

Question

Does A01604-2 anti-eNOS/NOS3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-05-15

Answer

It shows on the product datasheet, A01604-2 anti-eNOS/NOS3 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-05-15

Question

We have observed staining in rat umbilical vein. What should we do? Is anti-eNOS/NOS3 antibody supposed to stain umbilical vein positively?

T. Miller

Verified customer

Asked: 2018-01-04

Answer

According to literature umbilical vein does express NOS3. According to Uniprot.org, NOS3 is expressed in spleen, placenta, umbilical vein, platelet, hippocampus trachea, erythroleukemia, liver, among other tissues. Regarding which tissues have NOS3 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Hippocampus, and Trachea, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7509596, 7514568, 7515611, 15489334
Platelet, Pubmed ID: 7475956
Spleen, Pubmed ID: 12853948

Boster Scientific Support

Answered: 2018-01-04

Question

I see that the anti-eNOS/NOS3 antibody A01604-2 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-06-14

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-06-14

Question

Is this A01604-2 anti-eNOS/NOS3 antibody reactive to the isotypes of NOS3?

S. Jha

Verified customer

Asked: 2016-03-11

Answer

The immunogen of A01604-2 anti-eNOS/NOS3 antibody is E.coli-derived human eNOS/NOS3 recombinant protein (Position: Q1052-P1203). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-03-11

Question

I was wanting to use your anti-eNOS/NOS3 antibody for ELISA for rat umbilical vein on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat umbilical vein identification?

S. Parker

Verified customer

Asked: 2015-04-29

Answer

It shows on the product datasheet, A01604-2 anti-eNOS/NOS3 antibody has been validated for ELISA, Flow Cytometry, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat umbilical vein in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-04-29

Question

We want to test anti-eNOS/NOS3 antibody A01604-2 on rat umbilical vein for research purposes, then I may be interested in using anti-eNOS/NOS3 antibody A01604-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

A. Walker

Verified customer

Asked: 2014-12-15

Answer

The products we sell, including anti-eNOS/NOS3 antibody A01604-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-12-15

Question

We bought anti-eNOS/NOS3 antibody for WB on hippocampus trachea in a previous experiment. I am using mouse, and I plan to use the antibody for IHC-P next. I am interested in examining hippocampus trachea as well as erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for IHC-P?

S. Evans

Verified customer

Asked: 2013-05-15

Answer

I have checked the website and datasheets of our anti-eNOS/NOS3 antibody and I see that A01604-2 has been validated on mouse in both WB and IHC-P. Thus A01604-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-05-15

Question

I have a question about product A01604-2, anti-eNOS/NOS3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

R. Banerjee

Verified customer

Asked: 2013-04-29

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01604-2 anti-eNOS/NOS3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-04-29

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A01604-2

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A01604-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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