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Product Info Summary
| SKU: | PA1995 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Selenocysteine lyase SCLY Antibody Picoband®
SKU/Catalog Number
PA1995
BA3406-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Selenocysteine lyase SCLY Antibody catalog # PA1995. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Selenocysteine lyase SCLY Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1995)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SCLY.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1995 is reactive to SCLY in Human
Observed Molecular Weight
85-91 kDa
Calculated molecular weight
48.1 kDa
Background of SCLY
SCLY (Selenocysteine Lyase), is an enzyme that catalyzes the chemical reaction. This enzyme belongs to the family of lyases, specifically the class of carbon-sulfur lyases. The International Radiation Hybrid Mapping Consortium mapped the human SCLY gene to chromosome 2. Mihara et al. (2000) showed that mouse Scly catalyzed the conversion of L-selenocysteine to L-alanine. Scly activity required pyridoxal 5-prime phosphate, was specific to L-selenocysteine, and showed maximum reactivity at pH 9.0.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1995 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: U87 Cell, HELA Cell, 293T Cell, MCF-7 Cell, COLO320 Cell, HE1080 Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-SCLY antibody, PA1995, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: 293T Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: HE1080 Cell Lysate
Specific Publications For Anti-Selenocysteine lyase SCLY Antibody Picoband® (PA1995)
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Customer Q&As
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8 Customer Q&As for Anti-Selenocysteine lyase SCLY Antibody Picoband®
Question
Have you tested if PA1995 is suitable for Mouse sample in IP or WB experiments?
Verified customer
Asked: 2022-07-22
Answer
The Anti-Selenocysteine Lyase SCLY Antibody (PA1995) isn't suitable for mouse sample in WB. Our lab hasn't tested this antibody in IP as well.
Boster Scientific Support
Answered: 2022-07-25
Question
Will anti-SCLY antibody PA1995 work on feline WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
Our lab technicians have not validated anti-SCLY antibody PA1995 on feline. You can run a BLAST between feline and the immunogen sequence of anti-SCLY antibody PA1995 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-05
Question
Does anti-SCLY antibody PA1995 work for WB with brain?
R. Banerjee
Verified customer
Asked: 2018-11-19
Answer
According to the expression profile of brain, SCLY is highly expressed in brain. So, it is likely that anti-SCLY antibody PA1995 will work for WB with brain.
Boster Scientific Support
Answered: 2018-11-19
Question
We are currently using anti-SCLY antibody PA1995 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-23
Answer
The anti-SCLY antibody (PA1995) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-23
Question
I was wanting to use to test anti-SCLY antibody PA1995 on human brain for research purposes, then I may be interested in using anti-SCLY antibody PA1995 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Jha
Verified customer
Asked: 2018-01-30
Answer
The products we sell, including anti-SCLY antibody PA1995, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-SCLY antibody PA1995. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-10
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-10
Question
My question regarding product PA1995, anti-SCLY antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Banerjee
Verified customer
Asked: 2014-03-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1995 anti-SCLY antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-03-05
Question
Will PA1995 anti-SCLY antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Moore
Verified customer
Asked: 2013-02-21
Answer
It shows on the product datasheet, PA1995 anti-SCLY antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-02-21
