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- Table of Contents
GRIN2A antibody (PA1058-1) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of NMDAR2A/GRIN2A using anti-NMDAR2A/GRIN2A antibody (PA1058-1).
NMDAR2A/GRIN2A was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NMDAR2A/GRIN2A Antibody (PA1058-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
NMDAR2A/GRIN2A was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NMDAR2A/GRIN2A Antibody (PA1058-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-NMDAR2A/GRIN2A Antibody Picoband®
GRIN2A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
GRIN2A antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GRIN2A antibody was positively detected by IHC in:
rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-NMDAR2A/GRIN2A Antibody Picoband®
View all GRIN2A Antibodies
View all GRIN2A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NMDAR2A, different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.
Cross-Reactivity
No cross reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
180 kDa
Calculated Molecular Weight
165.3 kDa
Background of GRIN2A
GRIN2A is also known as N-methyl-D-aspartate receptor channel, subunit epsilon-1 (NMDAR2A). This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-NMDAR2A/GRIN2A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1058-1)
Protocols
Western Blot protocol for GRIN2A antibody
Immunohistochemistry (IHC) protocol for GRIN2A antibody
Publications
Application:
Species:
The selective disruption of the JNK2/Syntaxin-1A interaction by JGRi1 protects against NMDA-evoked toxicity in SH-SY5Y cells
Published: 2024-08-03
Journal: NEUROCHEMISTRY INTERNATIONAL
Application: IF
Impact Factor: 4.40
Species: Human
PMID: 39098765
Oral edaravone ameliorates behavioral deficits and pathologies in a valproic acid-induced rat model of autism spectrum disorder
Published: 2024-07-20
Journal: NEUROPHARMACOLOGY
Application: WB
Impact Factor: 4.60
Species: Rat
PMID: 39033904
CNTN1 Aggravates Neuroinflammation and Triggers Cognitive Deficits in Male Mice by Boosting Crosstalk between Microglia and Astrocytes
Published: 2023-10-01
Journal: Aging and Disease
Application: WB
Impact Factor: 7.40
Species: Mouse
PMID: 37196127
CNTN1 in the Nucleus Accumbens is Involved in Methamphetamine-Induced Conditioned Place Preference in Mice
Published: 2023-04-04
Journal: NEUROTOXICITY RESEARCH
Application: WB
Impact Factor: 3.70
Species: Mouse
PMID: 37014368
Xiao-Yao-San Formula Improves Cognitive Ability by Protecting the Hippocampal Neurons in Ovariectomized Rats
Published: 2020-06-19
Journal: Evidence-based Complementary and Alternative Medicine
Application: IHC
Impact Factor: 1.81
Species: Rat
PMID: 32655660
Impaired hippocampal synaptic plasticity and NR2A/2B expression ratio in remifentanil withdrawal rats
Published: 2016-01-14
Journal: NEUROTOXICOLOGY
Application: IHC
Impact Factor: 2.74
Species: Rat
PMID: 26777139
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In stock
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PA1058-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 19, 2026