Anti-Fatty Acid Synthase/FASN Antibody Picoband™

Boster Bio Anti-Fatty Acid Synthase/FASN Antibody Picoband™ catalog # PB9865. Tested in IHC, ICC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: PB9865
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-Fatty Acid Synthase/FASN Antibody Picoband™

See all Fatty Acid Synthase/FASN products

SKU/Catalog Number

PB9865

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Fatty Acid Synthase/FASN Antibody Picoband™ catalog # PB9865. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Fatty Acid Synthase/FASN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9865)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

PB9865 is reactive to FASN in Human

Applications

PB9865 is guaranteed for IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunocytochemistry,0.5-1μg/ml
Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For FASN (Source: Uniprot.Org, NCBI)

Uniprot ID

P49327

Gene ID

2194

Gene Name

FASN

Full Name

Fatty acid synthase

Weight

273.427kDa

Alternative Names

EC 2.3.1; EC 2.3.1.85; EC 6.3.3.1; FASN; FASshort chain dehydrogenase/reductase family 27X, member 1; Fatty Acid Synthase; MGC14367; MGC15706; OA-519; SDR27X1 FASN FAS, OA-519, SDR27X1 fatty acid synthase fatty acid synthase|short chain dehydrogenase/reductase family 27X, member 1|type I fatty acid synthase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FASN, check out the FASN Infographic

FASN infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FASN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9865 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lu C, Ma J, Cai D. Tumour Biol. 2017 Apr;39(4):1010428317697574. doi: 10.1177/1010428317697574. Increased HAGLR expression promotes non-small cell lung cancer proliferation and invasion via enhanced de novo lipogenesis.

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17 Customer Q&As for Anti-Fatty Acid Synthase/FASN Antibody Picoband™

Question

Our lab want to know about using your anti-Fatty Acid Synthase/FASN antibody for regulation of cholesterol biosynthetic process studies. Has this antibody been tested with western blotting on hepg2 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

Thank you for your inquiry. This PB9865 anti-Fatty Acid Synthase/FASN antibody is validated on hepg2 whole cell lysates, u87 whole cell lysates. It is guaranteed to work for IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-04-23

Question

Will anti-Fatty Acid Synthase/FASN antibody PB9865 work on primate WB with liver?

Z. Johnson

Verified customer

Asked: 2020-03-16

Answer

Our lab technicians have not validated anti-Fatty Acid Synthase/FASN antibody PB9865 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Fatty Acid Synthase/FASN antibody PB9865 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-16

Question

We are currently using anti-Fatty Acid Synthase/FASN antibody PB9865 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

The anti-Fatty Acid Synthase/FASN antibody (PB9865) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-24

Question

Is a blocking peptide available for product anti-Fatty Acid Synthase/FASN antibody (PB9865)?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

We do provide the blocking peptide for product anti-Fatty Acid Synthase/FASN antibody (PB9865). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-14

Question

Do you have a BSA free version of anti-Fatty Acid Synthase/FASN antibody PB9865 available?

Verified Customer

Verified customer

Asked: 2019-11-11

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Fatty Acid Synthase/FASN antibody PB9865 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-11

Question

I see that the anti-Fatty Acid Synthase/FASN antibody PB9865 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-02

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-02

Question

I was wanting to use to test anti-Fatty Acid Synthase/FASN antibody PB9865 on human lymphoblast for research purposes, then I may be interested in using anti-Fatty Acid Synthase/FASN antibody PB9865 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

The products we sell, including anti-Fatty Acid Synthase/FASN antibody PB9865, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-04

Question

Does PB9865 anti-Fatty Acid Synthase/FASN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

As indicated on the product datasheet, PB9865 anti-Fatty Acid Synthase/FASN antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-17

Question

Will anti-Fatty Acid Synthase/FASN antibody PB9865 work for ICC with lymphoblast?

P. Banerjee

Verified customer

Asked: 2019-06-17

Answer

According to the expression profile of lymphoblast, FASN is highly expressed in lymphoblast. So, it is likely that anti-Fatty Acid Synthase/FASN antibody PB9865 will work for ICC with lymphoblast.

Boster Scientific Support

Answered: 2019-06-17

Question

Is this PB9865 anti-Fatty Acid Synthase/FASN antibody reactive to the isotypes of FASN?

Verified Customer

Verified customer

Asked: 2018-09-18

Answer

The immunogen of PB9865 anti-Fatty Acid Synthase/FASN antibody is E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-09-18

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphoblast using anti-Fatty Acid Synthase/FASN antibody PB9865. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-08-30

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-08-30

Question

I have attached the WB image, lot number and protocol we used for lymphoblast using anti-Fatty Acid Synthase/FASN antibody PB9865. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-01-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-12

Question

Can you help my question with product PB9865, anti-Fatty Acid Synthase/FASN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-09-22

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9865 anti-Fatty Acid Synthase/FASN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-09-22

Question

We were happy with the WB result of your anti-Fatty Acid Synthase/FASN antibody. However we have been able to see positive staining in eye cytoplasm. using this antibody. Is that expected? Could you tell me where is FASN supposed to be expressed?

C. Johnson

Verified customer

Asked: 2016-08-31

Answer

According to literature, eye does express FASN. Generally FASN expresses in cytoplasm. Regarding which tissues have FASN expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 8022791
Brain, Pubmed ID: 7567999, 16625196
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065

Boster Scientific Support

Answered: 2016-08-31

Question

I was wanting to use your anti-Fatty Acid Synthase/FASN antibody for ICC for human lymphoblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymphoblast identification?

A. Johnson

Verified customer

Asked: 2016-01-21

Answer

It shows on the product datasheet, PB9865 anti-Fatty Acid Synthase/FASN antibody has been validated for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymphoblast in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-01-21

Question

We have seen staining in human melanoma. Are there any suggestions? Is anti-Fatty Acid Synthase/FASN antibody supposed to stain melanoma positively?

M. Carter

Verified customer

Asked: 2014-01-17

Answer

According to literature melanoma does express FASN. According to Uniprot.org, FASN is expressed in liver, brain, eye, b-cell lymphoma, lymphoblast, melanoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have FASN expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 8022791
Brain, Pubmed ID: 7567999, 16625196
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065

Boster Scientific Support

Answered: 2014-01-17

Question

We ordered your anti-Fatty Acid Synthase/FASN antibody for IHC on eye in a previous experiment. I am using human, and We are going to use the antibody for WB next. Our lab want to know about examining eye as well as lymphoblast in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

K. Li

Verified customer

Asked: 2013-04-26

Answer

I viewed the website and datasheets of our anti-Fatty Acid Synthase/FASN antibody and it appears that PB9865 has been tested on human in both IHC and WB. Thus PB9865 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-04-26

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PB9865
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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