Anti-BRCC45 BABAM2 Antibody

Product Name

Anti-BRCC45 BABAM2 Antibody

View all BRE Antibodies

SKU/Catalog Number

A32264

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-BRCC45 BABAM2 Antibody (Catalog # A32264). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

BRCC45 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-BRCC45 BABAM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32264)

Host

Rabbit

Contents

BRCC45 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

BRCC45 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC45. The immunogen is located within the last 50 amino acids of BRCC45.

Reactive Species

A32264 is reactive to BRE in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

43.6 kDa

Background of BRE

BRCC45 was initially suggested to be a housekeeping protein that is highly expressed in brain and reproductive organs. Later experiments indicated BRCC45 forms a complex with the breast and ovarian predisposition proteins BRCA1 and BRCA2 as well as RAD51 and BRCC36. This complex has a ubiquitin E3 ligase activity and is thought to enhance cellular survival following DNA damage. BRCC45 has also been suggested to function as a death receptor-associated anti-apoptotic protein by inhibiting the BID-induced activation of the mitochondrial apoptotic pathway. Higher levels of BRCC45 were detected in the majority of hepatocellular carcinomas, suggesting that BRCC45 may promote tumorigenesis when overexpressed. At least three isoforms of BRCC45 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A32264 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

BRCC45 antibody can be used for detection of BRCC45 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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