Anti-NADPH oxidase 5 NOX5 Antibody

NOX5 antibody

Boster Bio Anti-NADPH oxidase 5 NOX5 Antibody catalog # PA1947. Tested in IHC, ICC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: PA1947
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, ICC, WB

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Product Name

Anti-NADPH oxidase 5 NOX5 Antibody

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SKU/Catalog Number

PA1947

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NADPH oxidase 5 NOX5 Antibody catalog # PA1947. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NADPH oxidase 5 NOX5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1947)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human NOX5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1947 is reactive to NOX5 in Human

Applications

PA1947 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

86 kDa

Calculated molecular weight

86.439kDa

Background of NOX5

NOX5 (Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al. (2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al. (2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For NOX5 (Source: Uniprot.org, NCBI)

Gene Name

NOX5

Full Name

NADPH oxidase 5

Weight

86.439kDa

Alternative Names

EC 1.6.3.-; MGC149776; MGC149777; NADPH oxidase 5; NADPH oxidase, EF-hand calcium binding domain 5; NOX5A; NOX5B NOX5 NADPH oxidase 5 NADPH oxidase 5|NADPH oxidase, EF-hand calcium binding domain 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NOX5, check out the NOX5 Infographic

NOX5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NOX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1947 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Jafari N1, Kim H1,2, Park R1,2, Li L1, Jang M1,2, Morris AJ3, Park J1,2, Huang C PLoS One. 2017 Jan 18;12(1):e0170327. doi: 10.1371/journal.pone.0170327. eCollection 2017. CRISPR-Cas9 Mediated NOX4 Knockout Inhibits Cell Proliferation and Invasi...

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3 Customer Q&As for Anti-NADPH oxidase 5 NOX5 Antibody

Question

We are currently using anti-NOX5 antibody PA1947 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

The anti-NOX5 antibody (PA1947) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-15

Question

Would anti-NOX5 antibody PA1947 work on canine ICC with spleen testis?

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

Our lab technicians have not tested anti-NOX5 antibody PA1947 on canine. You can run a BLAST between canine and the immunogen sequence of anti-NOX5 antibody PA1947 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine spleen testis in ICC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-18

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney epithelium spleen using anti-NOX5 antibody PA1947. Let me know if you need anything else.

L. Rodriguez

Verified customer

Asked: 2014-01-09

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-01-09

Order DetailsPrice
PA1947

100μg

$370
PA1947-10ug

10μg sample (liquid)

$99
PA1947-Biotin

100 μg Biotin conjugated

$570
PA1947-Cy3

100 μg Cy3 conjugated

$570
PA1947-Dylight488

100 μg Dylight488 conjugated

$570
PA1947-Dylight550

100 μg Dylight550 conjugated

$570
PA1947-Dylight594

100 μg Dylight594 conjugated

$570
PA1947-FITC

100 μg FITC conjugated

$570
PA1947-HRP

100 μg HRP conjugated

$570
PA1947-APC

100 μg APC conjugated

$670
PA1947-PE

100 μg PE conjugated

$670
PA1947-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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