Human BMP-7 ELISA Kit PicoKine™

Human BMP-7 ELISA Kit PicoKine™ (96 Tests). Quantitate Human BMP7 in bone tissue, cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0884
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: bone tissue, cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Human BMP-7 ELISA Kit PicoKine™

See all BMP-7 products

SKU/Catalog Number

EK0884

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human BMP-7 ELISA Kit PicoKine™ (96 Tests). Quantitate Human BMP7 in bone tissue, cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human BMP-7 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0884)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S293-H431

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0884 is reactive to BMP7 in Human samples

Validated Sample Types

bone tissue, cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK0884 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0884-CAP Anti-Human BMP7 Pre-coated 96-well strip microplate 1
EK0884-ST Human BMP7 Standard 2 vials, 10 ng/tube
EK0884-DA Human BMP7 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0190.0840.1430.2910.4850.9791.7462.576

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect BMP-7, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of BMP-7 in samples: 19580189 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human BMP-7 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5626398253244937
Standard deviation4.0812.3654.014.6615.8657.15
CV (%)7.3%4.7%5.5%8.8%6.5%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of BMP-7 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 156495756543.25.9%
Sample 226328625528527213.554.9%
Sample 398292191694894126.232.7%
*number of samples for each test n=16.

Gene/Protein Information For BMP7 (Source: Uniprot.Org, NCBI)

Uniprot ID

P18075

Gene ID

655

Gene Name

BMP7

Full Name

Bone morphogenetic protein 7

Weight

49.313kDa

Superfamily

TGF-beta family

Alternative Names

BMP7; BMP-7; bone morphogenetic protein 7; Eptotermin alfa; OP-1; OP-1OP1; Osteogenic protein 1 BMP7 OP-1 bone morphogenetic protein 7 bone morphogenetic protein 7|osteogenic protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BMP7, check out the BMP7 Infographic

BMP7 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BMP7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0884 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of sequentially released BMP-2 and BMP-7 from PELA microcapsule-based scaffolds on the bone regeneration.

Wang Q, Xue Ml, Zhao Gq, Liu Mg, Ma Yn, Ma Y. Int J Ophthalmol. 2015 Feb 18;8(1):39-45. Doi: 10.3980/J.Issn.2222-3959.2015.01.07. Ecollection 2015. Form-Deprivation Myopia Induces Decreased Expression Of Bone Morphogenetic Protein-2, 5 In Guinea P...

Che Jh, Zhang Zr, Li Gz, Tan Wh, Bai Xd, Qu Fj. Knee Surg Sports Traumatol Arthrosc. 2010 Apr;18(4):496-503. Doi: 10.1007/S00167-009-0962-2. Epub 2009 Oct 24. Application Of Tissue-Engineered Cartilage With Bmp-7 Gene To Repair Knee Joint Cartilag...

Tu Qn, Dong H, Lu Fe. Chin J Integr Med. 2011 Aug;17(8):612-5. Doi: 10.1007/S11655-011-0825-9. Epub 2011 Aug 9. Effects Of Panax Notoginoside On The Nephropathy In Rats With Type 1 Diabetes Mellitus.

Qu Y, Du E, Zhang Y, Li S, Han R, Qiu M. Nephrourol Mon. 2012 Spring;4(2):466-9. Doi: 10.5812/Numonthly.2124. Epub 2012 Mar 1. Changes In The Expression Of Bone Morphogenetic Protein 7 And Tamm- Horsfall Protein In The Early Stages Of Diabetic Nep...

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5 Customer Q&As for Human BMP-7 ELISA Kit PicoKine™

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-06-19

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-06-19

Question

Q: can you recommend the dilution ratio of serum samples for detection of BMP-7 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-05-18

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the BMP-7 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-05-18

Question

Q: how do I analyze ELISA data? I measured BMP-7 level in plasma.

L. Moore

Verified customer

Asked: 2020-02-26

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-02-26

Question

Q: how to thaw whole blood sample for BMP-7 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BMP-7 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-01-14

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

R. Dayal

Verified customer

Asked: 2018-01-03

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-01-03

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