|Sample Size:||30ug for $99, contact us for details|
|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-NRF1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Nuclear respiratory factor 1(NRF1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-NRF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1948)|
|Replacement Item||This antibody may replace the following items: sc-28379|sc-13031|sc-30911|sc-365651|sc-721|sc-28379-X|sc-13031-X|sc-721-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human NRF1(272-288aa QHGREDLLYAFEDQQTQ), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Nuclear respiratory factor 1|
|Molecular Weight||53541 MW|
|Protein Function||Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.|
|Tissue Specificity||Ubiquitously expressed with strongest expression in skeletal muscle.|
|Sequence Similarities||Belongs to the NRF1/Ewg family.|
|Alternative Names||Nuclear respiratory factor 1;NRF-1;Alpha palindromic-binding protein;Alpha-pal;NRF1;|
|Research Areas|||epigenetics and nuclear signaling|transcription|polymerase associated factors|pol ii transcription| epigenetics and nuclear signaling|other factors|transcription factors| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial biogenesis|metabolic signaling pathways|nucleotide metabolism|molecular processes|mitochondrial transcription||
Background for Nuclear respiratory factor 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-NRF1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-NRF1 Antibody Images
Click the images to enlarge.
Lane 1: U2OS Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 75KD
Observed bind size: 75KD
IHC(P): Human Mammary Cancer Tissue
ICC: A549 Cell
IHC(P): Rat Intestine Tissue
IHC(P): Human Intestinal Cance Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,