Anti-Nuclear respiratory factor 1 NRF1 Antibody

Boster Bio Anti-Nuclear respiratory factor 1 NRF1 Antibody catalog # PA1948. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA1948
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-Nuclear respiratory factor 1 NRF1 Antibody

See all NRF1 products

SKU/Catalog Number

PA1948

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Nuclear respiratory factor 1 NRF1 Antibody catalog # PA1948. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nuclear respiratory factor 1 NRF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1948)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NRF1 (272-288aa QHGREDLLYAFEDQQTQ), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1948 is reactive to NRF1 in Human, Mouse, Rat

Applications

PA1948 is guaranteed for IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For NRF1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q16656

Gene ID

4899

Gene Name

NRF1

Full Name

Nuclear respiratory factor 1

Weight

53.541kDa

Superfamily

NRF1/Ewg family

Alternative Names

Alpha palindromic-binding protein; ALPHA-PAL; EWG; HBZ17; LCR-F1; NFE2L1; Nrf1; NRF-1; nuclear respiratory factor 1; TCF11 NRF1 ALPHA-PAL nuclear respiratory factor 1 nuclear respiratory factor 1|alpha palindromic-binding protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NRF1, check out the NRF1 Infographic

NRF1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NRF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1948 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Defect of mitochondrial respiratory chain is a mechanism of ROS overproduction in a rat model of alcoholic liver disease: role of zinc deficiency

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6 Customer Q&As for Anti-Nuclear respiratory factor 1 NRF1 Antibody

Question

We are currently using anti-NRF1 antibody PA1948 for human tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

The anti-NRF1 antibody (PA1948) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-28

Question

Is a blocking peptide available for product anti-NRF1 antibody (PA1948)?

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

We do provide the blocking peptide for product anti-NRF1 antibody (PA1948). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-02

Question

See below the WB image, lot number and protocol we used for placenta using anti-NRF1 antibody PA1948. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-17

Question

I see that the anti-NRF1 antibody PA1948 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-02-28

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-02-28

Question

Would PA1948 anti-NRF1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-02-15

Answer

As indicated on the product datasheet, PA1948 anti-NRF1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-02-15

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-NRF1 antibody PA1948. Let me know if you need anything else.

J. Evans

Verified customer

Asked: 2014-07-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-07-15

Size

Total: $280

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PA1948
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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