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- Table of Contents
NRF1 antibody (M01129-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of PC-3 cells using anti-NRF1 antibody (M01129-1).
Overlay histogram showing PC-3 cells stained with M01129-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-NRF1 Antibody (M01129-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing PC-3 cells stained with M01129-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-NRF1 Antibody (M01129-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-NRF1 Antibody Picoband® (monoclonal, 2G4)
NRF1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
NRF1 antibody was positively detected by WB in:
human Jurkat whole cell, human HL-60 whole cell, human K562 whole cell, human Raji whole cell, human SH-SY5Y whole cell, human THP-1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NRF1 antibody was positively detected by IHC in:
human rectal cancer tissue, human lung cancer tissue, human mammary cancer tissue, human testis cancer tissue, human testis cancer tissue, human testicular cancer tissue, human tonsil tissue, human gastric cancer tissue, human rectal cancer tissue, human appendicitis tissue, human appendicitis tissue, human bladder cancer tissue, human lung cancer tissue, human lung cancer tissue, human melanoma tissue, human ovarian cancer tissue, human renal carcinoma tissue, human skin cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
NRF1 antibody was positively detected by ICC/IF in:
MCF7 cell
NRF1 antibody was positively detected by FC in:
PC-3 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-NRF1 Antibody Picoband® (monoclonal, 2G4)
View all NRF1 Antibodies
View all NRF1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
2G4
Isotype
Mouse IgG2a
Immunogen
E. coli-derived human NRF1 recombinant protein (Position: D246-Q503).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
54-70 kDa
Calculated Molecular Weight
53.5 kDa
Background of NRF1
Nuclear respiratory factor 1, is also known as NRF1. This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.
Cite This Product
Anti-NRF1 Antibody Picoband® (monoclonal, 2G4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01129-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Sheep
100%
High Homology
Dog
99%
High Homology
Cow
99%
High Homology
Zebrafish
92%
High Homology
Drosophila
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NRF1 antibody
Immunohistochemistry (IHC) protocol for NRF1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for NRF1 antibody
Flow Cytometry (FC) protocol for NRF1 antibody
Publications
Application:
Species:
Nazmeen A,Chen G,Ghosh TK,Maiti S.Breast cancer pathogenesis is linked to the intra-tumoral estrogen sulfotransferase (hSULT1E1) expressions regulated by cellular redox dependent Nrf-2/NFκβ interplay.Cancer Cell Int.2020 Mar 4;20:70.doi:10.1186/s12935-020
Species: Human,Rat
PMID: 32158360
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Product Promise
We promise Anti-NRF1 Antibody Picoband® (monoclonal, 2G4) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M01129-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Nov 1, 2026