Product Info Summary
| SKU: | PA2039 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NTAL/LAT2 Antibody Picoband®
SKU/Catalog Number
PA2039
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NTAL/LAT2 Antibody catalog # PA2039. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NTAL/LAT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2039)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human NTAL.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2039 is reactive to LAT2 in Human
Observed Molecular Weight
29 kDa
Calculated molecular weight
26.6 kDa
Background of LAT2
LAT2 (Linker for Activation of T Cells Family, Member 2), also known as LAB or NTAL , is a protein that in humans is encoded by the LAT2 gene. By genomic sequence analysis, Doyle et al. (2000) mapped the WBSCR5 gene to chromosome 7q11.23. They noted that the flanking genes are highly conserved between mouse and human. By immunoprecipitation and immunoblot analysis, Brdicka et al. (2002) found that NTAL associated with GRB2, SOS1, and GAB1 after B-cell receptor activation. Janssen et al. (2003) determined that phosphorylation of LAB is likely mediated by SYK upon B-cell receptor stimulation. Binding analysis indicated associations with GRB2, and, indirectly, with PLCG1 and PLCG2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2039 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-NTAL antibody, PA2039, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human LAT2, 37.8KD
(162aa tag+ M1-A182)
Lane 1: Recombinant Human LAT2 Protein 10ng
Lane 2: Recombinant Human LAT2 Protein 5ng
Lane 3: Recombinant Human LAT2 Protein 2.5ng
Lane 4: Recombinant Human LAT2 Protein 1.25ng
Specific Publications For Anti-NTAL/LAT2 Antibody Picoband® (PA2039)
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1 Customer Q&As for Anti-NTAL/LAT2 Antibody Picoband®
Question
We are currently using anti-NTAL/LAT2 antibody PA2039 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
The anti-NTAL/LAT2 antibody (PA2039) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-17


