Data & Images
|Product Name||Anti-NTAL Antibody|
|Description||Rabbit IgG polyclonal antibody for Linker for activation of T-cells family member 2(LAT2) detection. Tested with WB in Human.|
|Cite This Product||Anti-NTAL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2039)|
|Replacement Item||This antibody may replace the following items: sc-133726|sc-27581|sc-27583 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human NTAL(34-48aa KRSEKIYQQRSLRED).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Linker for activation of T-cells family member 2|
|Molecular Weight||26550 MW|
|Protein Function||Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2. .|
|Tissue Specificity||Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B- cells, NK cells and monocytes. Absent from T-cells (at protein level). .|
|Subcellular Localization||Cell membrane ; Single-pass type III membrane protein . Present in lipid rafts.|
|Alternative Names||Linker for activation of T-cells family member 2;Linker for activation of B-cells;Membrane-associated adapter molecule;Non-T-cell activation linker;Williams-Beuren syndrome chromosomal region 15 protein;Williams-Beuren syndrome chromosomal region 5 protein;LAT2;LAB, NTAL, WBS15, WBSCR15, WBSCR5;HSPC046;|
|Research Areas|||neuroscience|neurotransmitter|amino acids| signal transduction|metabolism| metabolism|pathways and processes|metabolic signaling pathways|amino acid metabolism|types of disease|cancer||
Background for Linker for activation of T-cells family member 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-NTAL Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-NTAL Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant Human LAT2, 37.8KD (162aa tag+ M1-A182)
Lane 1: Recombinant Human LAT2 Protein 10ng
Lane 2: Recombinant Human LAT2 Protein 5ng
Lane 3: Recombinant Human LAT2 Protein 2.5ng
Lane 4: Recombinant Human LAT2 Protein 1.25ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,