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Product Info Summary
| SKU: | A04071 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-NTE PNPLA6 Antibody
SKU/Catalog Number
A04071
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NTE PNPLA6 Antibody catalog # A04071. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NTE PNPLA6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04071)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human PNPLA6. AA range:1031-1080
Reactive Species
A04071 is reactive to PNPLA6 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
151.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04071 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of AD293 cells using NTE Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from AD293 cells, using PNPLA6 Antibody.
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Immunohistochemical analysis of paraffin-embedded rat-testis, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded rat-testis, antibody was diluted at 1:100
Specific Publications For Anti-NTE PNPLA6 Antibody (A04071)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-NTE PNPLA6 Antibody
Question
I was wanting to use to test anti-NTE antibody A04071 on human brain for research purposes, then I may be interested in using anti-NTE antibody A04071 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
The products we sell, including anti-NTE antibody A04071, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-16
Question
Would anti-NTE antibody A04071 work for WB with brain?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
According to the expression profile of brain, PNPLA6 is highly expressed in brain. So, it is likely that anti-NTE antibody A04071 will work for WB with brain.
Boster Scientific Support
Answered: 2020-03-03
Question
Is a blocking peptide available for product anti-NTE antibody (A04071)?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
We do provide the blocking peptide for product anti-NTE antibody (A04071). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-27
Question
Does anti-NTE antibody A04071 work on feline IHC with blood?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
Our lab technicians have not validated anti-NTE antibody A04071 on feline. You can run a BLAST between feline and the immunogen sequence of anti-NTE antibody A04071 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline blood in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-05
Question
Here is the WB image, lot number and protocol we used for brain using anti-NTE antibody A04071. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-31
Question
We are currently using anti-NTE antibody A04071 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-NTE antibody (A04071) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-NTE antibody A04071. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-06
Question
I was wanting to use your anti-NTE antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
As indicated on the product datasheet, A04071 anti-NTE antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-09
Question
I see that the anti-NTE antibody A04071 works with WB, what is the protocol used to produce the result images on the product page?
K. Jackson
Verified customer
Asked: 2018-03-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-03-13
Question
Will A04071 anti-NTE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Brown
Verified customer
Asked: 2017-06-16
Answer
You can see on the product datasheet, A04071 anti-NTE antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-16
Question
Do you have a BSA free version of anti-NTE antibody A04071 available?
R. Li
Verified customer
Asked: 2015-11-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NTE antibody A04071 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-11-18
Question
Is this A04071 anti-NTE antibody reactive to the isotypes of PNPLA6?
M. Parker
Verified customer
Asked: 2013-04-09
Answer
The immunogen of A04071 anti-NTE antibody is Synthesized peptide derived from the Internal region of human NTE. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-04-09
Question
My question regarding product A04071, anti-NTE antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
F. Jha
Verified customer
Asked: 2013-01-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04071 anti-NTE antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-01
