Anti-Angiopoietin 2/ANGPT2 Antibody

Boster Bio Anti-Angiopoietin 2/ANGPT2 Antibody catalog # PA1005. Tested in WB applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: PA1005
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Angiopoietin 2/ANGPT2 Antibody

See all Angiopoietin-2 products

SKU/Catalog Number

PA1005

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Angiopoietin 2/ANGPT2 Antibody catalog # PA1005. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Angiopoietin 2/ANGPT2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1005)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Angiopoietin 2 (454-468aa NLNGMYYPQRQNTNK).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1005 is reactive to ANGPT2 in Human

Applications

PA1005 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ANGPT2 (Source: Uniprot.Org, NCBI)

Uniprot ID

O15123

Gene ID

285

Gene Name

ANGPT2

Full Name

Angiopoietin-2

Weight

56.919kDa

Alternative Names

AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand ANGPT2 AGPT2, ANG2 angiopoietin 2 angiopoietin-2|Tie2-ligand|angiopoietin-2B|angiopoietin-2a

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ANGPT2, check out the ANGPT2 Infographic

ANGPT2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ANGPT2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1005 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Benefical Therapeutic Effect of Chinese Herbal Xinji'erkang Formula on Hypertension-Induced Renal Injury in the 2-Kidney-1-Clip Hypertensive Rats

Li C, Fan J, Song X, Zhang B, Chen Y, Li C, Mi K, Ma H, Song Y, Tao X, Li G. Plos One. 2013 Sep 11;8(9):E75388. Doi: 10.1371/Journal.Pone.0075388. Ecollection 2013. Expression Of Angiopoietin-2 And Vascular Endothelial Growth Factor Receptor-3 Cor...

Bai X, Li X, Tian J, Zhou Z. Plos One. 2014 Apr 23;9(4):E96117. Doi: 10.1371/Journal.Pone.0096117. Ecollection 2014. Antiangiogenic Treatment Diminishes Renal Injury And Dysfunction Via Regulation Of Local Akt In Early Experimental Diabetes.

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7 Customer Q&As for Anti-Angiopoietin 2/ANGPT2 Antibody

Question

What is the isotype/subclass for this antibody?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

We use synthetic peptide as the immunogen, take rabbit serum and use affinity purification. We did not distinguish IG1-4 as monoclonal antibodies. Ig type of polyclonal antibodies may be one or several of IG1, 2, 3, 4.

Boster Scientific Support

Answered: 2020-05-01

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for substantia nigra using anti-Angiopoietin 2/ANGPT2 antibody PA1005. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-02

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-02

Question

I would like to test anti-Angiopoietin 2/ANGPT2 antibody PA1005 on human substantia nigra for research purposes, then I may be interested in using anti-Angiopoietin 2/ANGPT2 antibody PA1005 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

The products we sell, including anti-Angiopoietin 2/ANGPT2 antibody PA1005, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-20

Question

Will anti-Angiopoietin 2/ANGPT2 antibody PA1005 work for WB with substantia nigra?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

According to the expression profile of substantia nigra, ANGPT2 is highly expressed in substantia nigra. So, it is likely that anti-Angiopoietin 2/ANGPT2 antibody PA1005 will work for WB with substantia nigra.

Boster Scientific Support

Answered: 2019-11-19

Question

I have a question about product PA1005, anti-Angiopoietin 2/ANGPT2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-04-29

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1005 anti-Angiopoietin 2/ANGPT2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-04-29

Question

Is this PA1005 anti-Angiopoietin 2/ANGPT2 antibody reactive to the isotypes of ANGPT2?

Verified Customer

Verified customer

Asked: 2019-03-28

Answer

The immunogen of PA1005 anti-Angiopoietin 2/ANGPT2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Angiopoietin 2(454-468aa NLNGMYYPQRQNTNK). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-03-28

Question

Is a blocking peptide available for product anti-Angiopoietin 2/ANGPT2 antibody (PA1005)?

Verified Customer

Verified customer

Asked: 2018-05-09

Answer

We do provide the blocking peptide for product anti-Angiopoietin 2/ANGPT2 antibody (PA1005). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-05-09

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PA1005
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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