|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Angiopoietin-2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Angiopoietin-2(ANGPT2) detection. Tested with IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-Angiopoietin-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1037)|
|Replacement Item||This antibody may replace the following items: sc-20718|sc-6320|sc-7015|sc-7016|sc-7017|sc-74402|sc-74403|sc-8357 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||E.coli-derived human Angiopoietin 2 recombinant protein (Position: Y19-N348). Human Angiopoietin 2 shares 84% and 85% amino acid (aa) sequences identity with mouse and rat Angiopoietin 2, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||56919 MW|
|Protein Function||Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. .|
|Sequence Similarities||Contains 1 fibrinogen C-terminal domain.|
|Research Areas|||cardiovascular|angiogenesis|growth factors|angiopoietin||
Background for Angiopoietin-2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Angiopoietin-2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Angiopoietin-2 Antibody Images
Click the images to enlarge.
IHC(P): Human Placenta Tissue
IHC(P): Rat Intestine Tissue
IHC(P): Mouse Intestine Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,