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Product Info Summary
| SKU: | A05446 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NVL Antibody
SKU/Catalog Number
A05446
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NVL Antibody catalog # A05446. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NVL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05446)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from NVL . at AA range: 320-400
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05446 is reactive to NVL in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
95.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05446 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of extracts from rat stomach, 293 cells, using NVL Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Specific Publications For Anti-NVL Antibody (A05446)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-NVL Antibody
Question
My question regarding product A05446, anti-NVL antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05446 anti-NVL antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-17
Question
We are currently using anti-NVL antibody A05446 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The anti-NVL antibody (A05446) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-29
Question
Is this A05446 anti-NVL antibody reactive to the isotypes of NVL?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The immunogen of A05446 anti-NVL antibody is Synthesized peptide derived from the Internal region of human NVL. at AA rangle: 320-400. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-21
Question
Does anti-NVL antibody A05446 work on pig WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
Our lab technicians have not tested anti-NVL antibody A05446 on pig. You can run a BLAST between pig and the immunogen sequence of anti-NVL antibody A05446 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-03
Question
Would A05446 anti-NVL antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
You can see on the product datasheet, A05446 anti-NVL antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-NVL antibody A05446. Let me know if you need anything else.
N. Parker
Verified customer
Asked: 2013-12-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-23
Question
I see that the anti-NVL antibody A05446 works with WB, what is the protocol used to produce the result images on the product page?
E. Kulkarni
Verified customer
Asked: 2013-05-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-05-28
