Anti-Apolipoprotein A-IV ApoA4 Antibody

Product Name

Anti-Apolipoprotein A-IV ApoA4 Antibody

View all Apolipoprotein A-IV/ApoA4 Antibodies

SKU/Catalog Number

A01973

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Apolipoprotein A-IV ApoA4 Antibody (Catalog # A01973). Tested in ELISA, WB applications. This antibody reacts with Human, Chicken.

Storage & Handling

ApoA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Apolipoprotein A-IV ApoA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01973)

Host

Rabbit

Contents

ApoA4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

ApoA4 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ApoA4. The immunogen is located within amino acids 320 - 370 of ApoA4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

SRPK1 antibody is human, mouse and rat reactive. Multiple isoforms of SRPK1 are known to exist; this antibody will detect all except isoforms b and e. SRPK1 antibody is predicted to not cross-react with SRPK2.

Reactive Species

A01973 is reactive to APOA4 in Chicken, Human

Applications

A01973 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

45.399kDa

Background of Apolipoprotein A-IV/ApoA4

Apolipoprotein A4 (also known as ApoA-IV) is a plasma protein that is an O-linked glycoprotein after proteolytic processing. ApoA4 is a major constituent of HDL and it also can be found on the surface of newly synthesized chylomicrons. The physiological function of ApoA4 is not completely identified so far, but evidence from several in vitro studies indicate that this apolipoprotein is involved in several steps of the reverse cholesterol transport pathway, which transports cholesterol from peripheral cells back to the liver.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ApoA4 antibody can be used for detection of ApoA4 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of ApoA4 in human testis tissue lysate with ApoA4 antibody at (A) 0.5 and (B) 1 μg/mL.

Gene/Protein Information For APOA4 (Source: Uniprot.org, NCBI)

Alternative Names

apo-AIV; apoA-IV; Apolipoprotein A4; Apolipoprotein AIV; Apolipoprotein A-IV; MGC142154; MGC142156 APOA4 apolipoprotein A4 apolipoprotein A-IV|apo-AIV|apoA-IV

For more info on APOA4, check out the APOA4 Infographic

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In this infographic, you will see the following information for APOA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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