Anti-Glucose Transporter GLUT4/SLC2A4 Antibody

Rabbit Polyclonal Glucose Transporter GLUT4/SLC2A4 Antibody. Validated in IHC-P, WB and tested in Human, Mouse, Rat.

SKU PA1722
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC-P, WB

Overview

Product Name Anti-Glucose Transporter GLUT4/SLC2A4 Antibody
See all SLC2A4 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1722
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit Polyclonal Glucose Transporter GLUT4/SLC2A4 Antibody. Validated in IHC-P, WB and tested in Human, Mouse, Rat. Size: 100μg/vial.
Cite This Product Anti-Glucose Transporter GLUT4/SLC2A4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1722)
Specificity Anti-Glucose Transporter GLUT4/SLC2A4 Antibody (PA1722) reacts with Human, Mouse, Rat SLC2A4, in native form and recombinant. Superfamily members of SLC2A4 are not reactive to PA1722.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen Anti-Glucose Transporter GLUT4/SLC2A4 Antibody (PA1722) was raised against A synthetic peptide corresponding to a sequence at the C-terminus of human GLUT4 (491-509aa EQEVKPSTELEYLGPDEND), identical to the related rat and mouse sequences..
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-Glucose Transporter GLUT4/SLC2A4 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-Glucose Transporter GLUT4/SLC2A4 Antibody (PA1722).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

/antibody/pa1722 1 IHC anti glut4 antibody.jpg

Anti-GLUT4 antibody, PA1722, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue

/antibody/pa1722 2 WB anti glut4 antibody.jpg

Anti-GLUT4 antibody, PA1722, Western blotting
All lanes: Anti GLUT4 (PA1722) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 55 KD
Observed bind size: 55 KD

/p/a/pa1722 3.jpg

Figure 3. IHC analysis of GLUT4 using anti- GLUT4 antibody (PA1722).
GLUT4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- GLUT4 Antibody (PA1722) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P14672
Gene Name SLC2A4
Protein Name Solute carrier family 2, facilitated glucose transporter member 4
Tissue Specificity Skeletal and cardiac muscles; brown and white fat.
Alternative Names Solute carrier family 2, facilitated glucose transporter member 4;Glucose transporter type 4, insulin-responsive;GLUT-4;SLC2A4;GLUT4;Show more
Subcellular Localization Cell membrane ; Multi-pass membrane protein . Endomembrane system ; Multi-pass membrane protein . Cytoplasm, perinuclear region . Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
Molecular Weight 54787 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Insulin-regulated facilitative glucose transporter.
Research Areas Cardiovascular, Energy Metabolism, Energy Transfer Pathways, Epigenetics And Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Signal Transduction, Transcription, Transcription Factors

*You can search these to find other products in these research areas.
Background Facilitated glucose transport by mammalian cells is not a property of a single protein but an activity associated with a family of structurally related proteins. Glucose transporter 4 is a insulin-responsive glucose transporter. It belongs to solute carrier family 2, member 1. Insulin alters the subcellular localization of GLUT4 vesicles in human muscle, and that this effect is impaired equally in insulin-resistant subjects with and without diabetes. A similar pattern of defects cause insulin resistance in human adipocytes.Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Publishing with Anti-Glucose Transporter GLUT4/SLC2A4 Antibody (PA1722)? Please let us know so we can cite the reference in this product datasheet. Email us at support@bosterbio.com.

Order Product (PA1722)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280

USD $280

Ships next business day.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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Publications

Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model
lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPAR? pathway in hepatocellular carcinoma
Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats
Zhou X, Wu W, Chen J, Wang X, Wang Y. Nutr Metab (Lond). 2015 Mar 8;12:10. Doi: 10.1186/S12986-015-0006-5. Ecollection 2015. Amp-Activated Protein Kinase Is Required For The Anti-Adipogenic Effects Of Alpha-Linolenic Acid.

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to glut4 antibody, glut 4 antibody, glut-4 antibody