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Product Info Summary
| SKU: | A06461-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GLUT10/SLC2A10 Antibody Picoband®
SKU/Catalog Number
A06461-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GLUT10/SLC2A10 Antibody Picoband® catalog # A06461-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GLUT10/SLC2A10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06461-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GLUT10/SLC2A10 recombinant protein (Position: A32-Q511).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06461-2 is reactive to SLC2A10 in Human
Observed Molecular Weight
67 kDa
Calculated molecular weight
56.9 kDa
Background of SLC2A10
Solute carrier family 2, facilitated glucose transporter member 10 is a protein that in humans is encoded by the SLC2A10 gene. It is mapped to 20q13.12. This gene encodes a member of the class III facilitative glucose transporter family. The encoded protein plays a role in regulation of glucose homeostasis. Mutations in this gene have been associated with arterial tortuosity syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06461-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HepG2 whole cell
Validation Images & Assay Conditions
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Western blot analysis of GLUT10/SLC2A10 using anti-GLUT10/SLC2A10 antibody (A06461-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT10/SLC2A10 antigen affinity purified polyclonal antibody (Catalog # A06461-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT10/SLC2A10 at approximately 67KD. The expected band size for GLUT10/SLC2A10 is at 54KD.
Specific Publications For Anti-GLUT10/SLC2A10 Antibody Picoband® (A06461-2)
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