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- Table of Contents
CDKN1A antibody (M00145-3) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Designing a Western blot for CDKN1A? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CDKN1A Western Blot GuideBundle Save
Anti-p21 CDKN1A Rabbit Monoclonal Antibody
CDKN1A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
CDKN1A antibody was positively detected by IHC in:
human colon tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
Human
Recommend
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-p21 CDKN1A Rabbit Monoclonal Antibody
View all CDKN1A Antibodies
View all CDKN1A Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p21
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
21 kDa
Calculated Molecular Weight
18.1 kDa
Cite This Product
Anti-p21 CDKN1A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00145-3)
Protocols
Immunohistochemistry (IHC) protocol for CDKN1A antibody
Publications
Application:
Species:
Short-Term DMOG treatment rejuvenates senescent mesenchymal stem cells by enhancing mitochondrial function and mitophagy through the HIF-1α/BNIP3 pathway
Published: 2025-06-02
Journal: Stem Cell Research & Therapy
Application: WB
Impact Factor: 7.30
Species: Human
PMID: 40457488
Structural characterization and effect of activating autophagy and regulating oxidative stress of polysaccharide from fibrous roots of Atractylodes chinensis
Published: 2025-05-18
Journal: BIOORGANIC CHEMISTRY
Application: WB
Impact Factor: 4.70
Species: Human
PMID: 40412222
PHLDA2 overexpression facilitates senescence and apoptosis via the mitochondrial route in human nucleus pulposus cells by regulating Wnt/β-catenin signalling pathway
Published: 2024-05-09
Journal: IUBMB LIFE
Application: IHC,WB
Impact Factor: 3.70
Species: Human
PMID: 38721892
Vitamin C Inhibited Pulmonary Metastasis through Activating Nrf2/HO-1 Pathway
Published: 2024-02-28
Journal: MOLECULAR NUTRITION & FOOD RESEARCH
Application: WB
Impact Factor: 4.50
Species: Mouse
PMID: 38419398
p21 Prevents the Exhaustion of Cluster of Differentiation 4-Positive T Cells Within the Antitumor Immune Response Against Colorectal Cancer
Published: 2023-09-19
Journal: GASTROENTEROLOGY
Application: FC,IHC
Impact Factor: 29.40
Species: Mouse,Human
PMID: 37734420
Angelica sinensis polysaccharides alleviate the oxidative burden on hematopoietic cells by restoring 5-fluorouracil-induced oxidative damage in perivascular mesenchymal progenitor cells
Published: 2023-05-06
Journal: PHARMACEUTICAL BIOLOGY
Application: WB
Impact Factor: 3.80
Species: Mouse
PMID: 37148130
Synthesis, biological activity and mechanism of action of novel allosecurinine derivatives as potential antitumor agents
Published: 2023-03-08
Journal: BIOORGANIC & MEDICINAL CHEMISTRY
Application: WB
Impact Factor: 3.50
Species: Human
PMID: 36906964
Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration
Published: 2021-04-26
Journal: Cancer Cell International
Application: WB
Impact Factor: 5.72
Species: Human
PMID: 33902600
Leucine aminopeptidase 3 promotes migration and invasion of breast cancer cells through upregulation of fascin and matrix metalloproteinases-2/9 expression
Published: 2018-11-11
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 2.96
Species: Human
PMID: 30417585
Review
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Product Promise
We promise Anti-p21 CDKN1A Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00145-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 21, 2026