Anti-14-3-3 sigma/SFN Antibody Picoband™

14-3-3 sigma/Stratifin antibody

Boster Bio Anti-14-3-3 sigma/SFN Antibody Picoband™ catalog # A01127. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A01127
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-14-3-3 sigma/SFN Antibody Picoband™

View all 14-3-3 sigma/Stratifin Antibodies

SKU/Catalog Number

A01127

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-14-3-3 sigma/SFN Antibody Picoband™ catalog # A01127. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-14-3-3 sigma/SFN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01127)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01127 is reactive to SFN in Human, Mouse, Rat

Applications

A01127 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

27.774kDa

Background of 14-3-3 sigma/Stratifin

Stratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Immunofluorescence, 2μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For SFN (Source: Uniprot.org, NCBI)

Gene Name

SFN

Full Name

14-3-3 protein sigma

Weight

27.774kDa

Superfamily

14-3-3 family

Alternative Names

14-3-3 protein sigma; 1433 sigma; 14-3-3 sigma; Epithelial cell marker protein 1; HME1; SFN; stratifin; YWHAS SFN YWHAS stratifin 14-3-3 protein sigma|epithelial cell marker protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SFN, check out the SFN Infographic

SFN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SFN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01127 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Makorin-2 Is a Neurogenesis Inhibitor Downstream of Phosphatidylinositol\n 3-Kinase/Akt (PI3K/Akt)\n Signal

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Order DetailsPrice
A01127

100μg

$370
A01127-10ug

10μg sample (liquid)

$99
A01127-Biotin

100 μg Biotin conjugated

$570
A01127-Cy3

100 μg Cy3 conjugated

$570
A01127-Dylight488

100 μg Dylight488 conjugated

$570
A01127-Dylight550

100 μg Dylight550 conjugated

$570
A01127-Dylight594

100 μg Dylight594 conjugated

$570
A01127-FITC

100 μg FITC conjugated

$570
A01127-HRP

100 μg HRP conjugated

$570
A01127-APC

100 μg APC conjugated

$670
A01127-PE

100 μg PE conjugated

$670
A01127-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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