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Product Info Summary
| SKU: | PB9304 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-P2RX4 Antibody Picoband®
SKU/Catalog Number
PB9304
PB0337 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-P2RX4 Antibody Picoband® catalog # PB9304. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P2RX4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9304)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human P2RX4 recombinant protein (Position: N262-Q388). Human P2RX4 shares 91% and 90% amino acid (aa) sequence identity with mouse and rat P2RX4, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9304 is reactive to P2RX4 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
43.4 kDa
Background of P2RX4
Purinoceptor P2X4, also called P2X4R is a protein that in humans is encoded by the P2RX4 gene. This gene belongs to the family of purinoceptors for ATP. P2RX4 was mapped to 12q24.32 by fluorescence in situ hybridization. P2RX4 is a receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. P2X4 receptor in hyperactive microglia is necessary for tactile allodynia after nerve injury and is sufficient to produce tactile allodynia in normal animals. P2X4 recetors have beeb implicated in the regulation of cardiac function, ATP-mediated cell death, synaptic strengthening, and activating of the inflammasome in response to injury.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9304 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat, Monkey
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human A375 whole cell, human T-47D whole cell, human A549 whole cell, monkey COS-7 whole cell, rat NRK whole cell, rat RH-35 whole cell, mouse kidney tissue, mouse liver tissue, mouse Raw2647 whole cell, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
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Western blot analysis of P2RX4 using anti-P2RX4 antibody (PB9304).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A375 whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: monkey COS-7 whole cell lysates,
Lane 7: rat NRK whole cell lysates,
Lane 8: rat RH-35 whole cell lysates,
Lane 9: mouse kidney tissue lysates,
Lane 10: mouse liver tissue lysates,
Lane 11: mouse Raw264.7 whole cell lysates,
Lane 12: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2RX4 antigen affinity purified polyclonal antibody (Catalog # PB9304) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2RX4 at approximately 72KD. The expected band size for P2RX4 is at 43KD.
Specific Publications For Anti-P2RX4 Antibody Picoband® (PB9304)
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1 Customer Q&As for Anti-P2RX4 Antibody Picoband®
Question
We are currently using anti-P2RX4 antibody PB9304 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The anti-P2RX4 antibody (PB9304) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-18
