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Product Info Summary
|Reactive Species:||Human, Monkey, Mouse, Rat|
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Anti-P2RX4 Antibody Picoband™
Boster Bio Anti-P2RX4 Antibody Picoband™ catalog # PB9304. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P2RX4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9304)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human P2RX4 recombinant protein (Position: N262-Q388). Human P2RX4 shares 91% and 90% amino acid (aa) sequence identity with mouse and rat P2RX4, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PB9304 is reactive to P2RX4 in Human, Monkey, Mouse, Rat
PB9304 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat, Monkey
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of P2RX4 using anti-P2RX4 antibody (PB9304).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A375 whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: monkey COS-7 whole cell lysates,
Lane 7: rat NRK whole cell lysates,
Lane 8: rat RH-35 whole cell lysates,
Lane 9: mouse kidney tissue lysates,
Lane 10: mouse liver tissue lysates,
Lane 11: mouse Raw264.7 whole cell lysates,
Lane 12: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2RX4 antigen affinity purified polyclonal antibody (Catalog # PB9304) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2RX4 at approximately 72KD. The expected band size for P2RX4 is at 43KD.
Gene/Protein Information For P2RX4 (Source: Uniprot.Org, NCBI)
P2X purinoceptor 4
P2X receptor family
ATP receptor; ATP-gated cation channel protein; P2RX4; P2X purinoceptor 4; P2X4; P2X4P2X receptor, subunit 4; P2X4R; purinergic receptor P2X, ligand-gated ion channel, 4; Purinergic receptor; purinoceptor P2X4 P2RX4 P2X4, P2X4R purinergic receptor P2X 4 P2X purinoceptor 4|ATP receptor|ATP-gated cation channel protein|P2X receptor, subunit 4|purinergic receptor P2X, ligand gated ion channel, 4|purinergic receptor P2X4|purinoceptor P2X4*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on P2RX4, check out the P2RX4 Infographic
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In this infographic you will see the following information for P2RX4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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