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Product Info Summary
| SKU: | P00176-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-p38 MAPK Phospho (pT180/pY182) MAPK14 Rabbit Monoclonal Antibody, Clone#RM243
SKU/Catalog Number
P00176-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-p38 MAPK Phospho (pT180/pY182) MAPK14 Rabbit Monoclonal Antibody, Clone#RM243 (Catalog # P00176-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p38 MAPK Phospho (pT180/pY182) MAPK14 Rabbit Monoclonal Antibody, Clone#RM243 (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00176-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM243
Isotype
Rabbit IgG
Immunogen
A phospho-peptide corresponding to human Phospho-p38 MAPK (Thr180/Tyr182)
Cross-reactivity
This antibody reacts to p38 MAPK only when dual phosphorylation at Thr180 and Tyr182 is observed. There is no cross-reactivity with p38 MAPK without dual phosphorylation at Thr180 and Tyr182.
Reactive Species
P00176-1 is reactive to MAPK14 in Human
Calculated molecular weight
41.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00176-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution
Validation Images & Assay Conditions
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Western Blot of HeLa cell lysates, treated or non- treated with anisomycin using Anti-Phospho-p38 MAPK (Thr180/Tyr182) RM243 at a 1:2000 dilution, showed a band of Phospho-p38 MAPK (~38 kDa) in anisomycin-treated HeLa cells.
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Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-p38 MAPK (Thr180/ Tyr182) RM243 at a 1:1000 dilution.
Specific Publications For Anti-p38 MAPK Phospho (pT180/pY182) MAPK14 Rabbit Monoclonal Antibody, Clone#RM243 (P00176-1)
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Customer Q&As
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16 Customer Q&As for Anti-p38 MAPK Phospho (pT180/pY182) MAPK14 Rabbit Monoclonal Antibody, Clone#RM243
Question
Is a blocking peptide available for product anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 (P00176-1)?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
We do provide the blocking peptide for product anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 (P00176-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-17
Question
We are currently using anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 P00176-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
The anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 (P00176-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-09
Question
Please see the WB image, lot number and protocol we used for renal cell carcinoma using anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 P00176-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-27
Question
My question regards to test anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 P00176-1 on human renal cell carcinoma for research purposes, then I may be interested in using anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 P00176-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
The products we sell, including anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 P00176-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-16
Question
My question regarding product P00176-1, anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Collins
Verified customer
Asked: 2019-01-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00176-1 anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-07
Question
I was wanting to use your anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 for IHC for human renal cell carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human renal cell carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-11-16
Answer
It shows on the product datasheet, P00176-1 anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human renal cell carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-16
Question
I see that the anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 P00176-1 works with IHC, what is the protocol used to produce the result images on the product page?
E. Krishna
Verified customer
Asked: 2018-07-12
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-07-12
Question
Is there a BSA free version of anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 P00176-1 available?
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 P00176-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-20
Question
We have seen staining in human renal cell carcinoma. Are there any suggestions? Is anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 supposed to stain renal cell carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-02-23
Answer
According to literature renal cell carcinoma does express MAPK14. According to Uniprot.org, MAPK14 is expressed in blood, peripheral blood, liver, b-cell, renal cell carcinoma, placenta, platelet, hepatoma, embryonic kidney, cervix carcinoma, leukemic t-cell, t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MAPK14 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 10727080
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Hepatoma, Pubmed ID: 10943842
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 7696354, 24275569
Peripheral blood, Pubmed ID: 7997261
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801, 18088087
Renal cell carcinoma, Pubmed ID: 11866441
T-cell, Pubmed ID: 21586573
Boster Scientific Support
Answered: 2018-02-23
Question
We ordered your anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
I have checked the website and datasheets of our anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2017-12-28
Question
I would like using your anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 for positive regulation of cyclase activity studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
Z. Anderson
Verified customer
Asked: 2017-07-03
Answer
We appreciate your inquiry. This P00176-1 anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 is validated on hela cells, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-07-03
Question
My boss were happy with the WB result of your anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone
P. Johnson
Verified customer
Asked: 2016-10-06
Answer
According to literature, liver does express MAPK14. Generally MAPK14 expresses in cytoplasm. Regarding which tissues have MAPK14 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 10727080
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Hepatoma, Pubmed ID: 10943842
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 7696354, 24275569
Peripheral blood, Pubmed ID: 7997261
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801, 18088087
Renal cell carcinoma, Pubmed ID: 11866441
T-cell, Pubmed ID: 21586573
Boster Scientific Support
Answered: 2016-10-06
Question
Would anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 P00176-1 work for IHC with renal cell carcinoma?
C. Moore
Verified customer
Asked: 2016-05-25
Answer
According to the expression profile of renal cell carcinoma, MAPK14 is highly expressed in renal cell carcinoma. So, it is likely that anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 P00176-1 will work for IHC with renal cell carcinoma.
Boster Scientific Support
Answered: 2016-05-25
Question
Will P00176-1 anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Krishna
Verified customer
Asked: 2015-01-08
Answer
As indicated on the product datasheet, P00176-1 anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-01-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for renal cell carcinoma using anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 P00176-1. Let me know if you need anything else.
G. Edwards
Verified customer
Asked: 2014-12-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-12-23
Question
Is this P00176-1 anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 reactive to the isotypes of MAPK14?
O. Krishna
Verified customer
Asked: 2014-02-03
Answer
The immunogen of P00176-1 anti-p38 MAPK Phospho (pT180/pY182) Rabbit Monoclonal antibody, Clone#RM243 is A phospho-peptide corresponding to human Phospho-p38 MAPK (Thr180/Tyr182). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-02-03
