Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase PAK 1(PAK1) detection. Tested with WB in Human;Mouse;Rat.
Anti-PAK1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-PAK1 Antibody
See all Pak1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for PAK1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. PAK1 information: Molecular Weight: 60647 MW; Subcellular Localization: Cytoplasm. Cell junction, focal adhesion. Cell membrane. Cell projection, ruffle membrane. Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin.|
|Cite This Product||Anti-PAK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1563)|
|Similar Products From
|Anti-PAK1 Antibody may replace the following items: sc 31683|sc 31684|sc 31685.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human PAK1(1-14aa MSNNGLDIQDKPPA), different from the related mouse and rat sequences by on amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For PAK1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||5058|
|Species Of This Entry||Human|
|Protein Name||Serine/threonine-protein kinase PAK 1|
|Superfamily||protein kinase superfamily|
|Alternative Names||Pak1|alpha-PAK; EC 2.7.11; EC 188.8.131.52; MGC130000; MGC130001; p21 protein (Cdc42/Rac)-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21-activated kinase 1; p65-PAK; PAK1; PAK-1; PAKalpha; serine/threonine-protein kinase PAK 1; STE20 homolog, yeast|
|Post Tranlational Modifications||Phosphorylation; Dephosphorylation; Cleavage; Myristoylation; Ribosylation; Methylation; Amidation; Ubiquitination; Acetylation; Demethylation; Prenylation; Nitration; Deacetylation; Oxidation|
|Subcellular Localization||Cytoplasm. Cell junction, focal adhesion. Cell membrane. Cell projection, ruffle membrane. Cell projection, invadopodium. Nucleus, nucleoplasm. Chromosome. Colocalizes with RUFY3, F-actin and other core migration components in invadopodia at the cell periphery (PubMed:25766321). Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin. Upon DNA damage, translocates to the nucleoplasm when phosphorylated at Thr-212 where is co-recruited with|
|Gene Location||11q13.5-q14.1, on Chromasome 11, gene sequence: NC_000011.10 (77322017..77514957, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For PAK1 (Source: Uniprot.org, NCBI)
|Protein Function||Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2- induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F- actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. Plays a role in RUFY3-mediated facilitating gastric cancer cells migration and invasion (PubMed:25766321).|
|Tissue Specificity||Overexpressed in gastric cancer cells and tissues (at protein level) (PubMed:25766321).|
|Interacting Proteins||ARHGEF7; CDC42; Cdc42; CDK11B; CRIPAK; GIT1; LIMK1; NCK2; PCBP1; RAC1; Rac1; RHOU|
|Research Areas||Apoptosis; Breast Cancer; Cancer; Neuroscience; Signal Transduction|
|Related Diseases||Malignant Neoplasms; Neoplasms; Tissue Adhesions; Malignant Neoplasm Of Breast; Mammary Neoplasms; Carcinoma; Cell Invasion; Cell Transformation, Neoplastic; Neoplasm Metastasis; Tumor Angiogenesis; Malignant Paraganglionic Neoplasm; Neoplasm Invasiveness; Neurofibromatoses; Hiv Infections; Immunologic Deficiency Syndromes; Tumor Progression; Hyperplasia; Malignant Neoplasm Of Prostate; Melanoma; Inflammation|
|Related Pathways||Cell Migration; Cell Motility; Localization; Cell Proliferation; Cell Growth; Cell Cycle; Angiogenesis; Pathogenesis; Cell Adhesion; Mitosis; Wound Healing; Secretion; Chemotaxis; Endocytosis; Rna Interference; Cell Division; Cytokinesis; Phagocytosis; Macropinocytosis; Mating|
|Background||Serine/threonine-protein kinase PAK 1 is an enzyme that in humans is encoded by the PAK1 gene. PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activated kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternative transcripts of this gene have been found, but their full-length natures have not yet been determined. The PAK1 gene is mapped to 11q13-q14 by inclusion within a mapped clone.|
|Scientific References||PMID: 8805275 by Brown J.L., et al. Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway.|
PMID: 9395435 by Sells M.A., et al. Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells.
Our Boster Quality Guarantee for Anti-PAK1 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: SW620 Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: CEM Cell Lysate
Lane 6: HT1080 Cell Lysate
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