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- Table of Contents
LAMP1 antibody (PA1822) performs well in IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IHC analysis of LAMP1 using anti-LAMP1 antibody (PA1822).
LAMP1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LAMP1 Antibody (PA1822) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
LAMP1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LAMP1 Antibody (PA1822) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-LAMP1 Antibody Picoband®
LAMP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC, WB |
Tested Applications
LAMP1 antibody was positively detected by WB in:
human Caco-2 whole cell, human Hela whole cell, human U2OS whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LAMP1 antibody was positively detected by IHC in:
human tonsil tissue, human liver cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-LAMP1 Antibody Picoband®
View all LAMP1 Antibodies
View all LAMP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human LAMP1, different from the related mouse and rat sequences by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90-120 kDa (Glycosylation)
Calculated Molecular Weight
44.9 kDa
Background of LAMP1
LAMP1 (lysosomal-associated membrane protein 1) also called LAMPA, LGP120 or CD107A, is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. CD107a has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells. By means of in situ hybridization, Mattei et al. (1990) assigned the LAMP1 gene to chromosome 13q34. A related gene, which may be a pseudogene, mapped to chromosome 12p13.3. Hybridization of LAMP1 cDNA to chromosome 12p13.3 was observed even when probes representing different portions of the LAMP1 cDNA were used.
Cite This Product
Anti-LAMP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1822)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
93%
High Homology
Rat
93%
High Homology
Rabbit
93%
High Homology
Cow
93%
High Homology
Sheep
93%
High Homology
Dog
93%
High Homology
Zebrafish
87%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LAMP1 antibody
Immunohistochemistry (IHC) protocol for LAMP1 antibody
Publications
Application:
Species:
Lysosomal dysfunction contributes to lead (Pb) and High-Fat Diet (HFD)-induced neuronal apoptosis: a link with cathepsin B
Published: 2026-04-28
Journal: NEUROTOXICOLOGY
Application: WB
Impact Factor: 3.90
Species: Rat
PMID: 42061594
3, 14, 19-Triacetyl Andrographolide alleviates the cognitive dysfunction of 3 × Tg-AD mice by inducing initiation and promoting degradation process of autophagy
Published: 2022-09-17
Journal: PHYTOTHERAPY RESEARCH
Application: WB
Impact Factor: 6.39
Species: Mouse
PMID: 36114804
Synergistic Lysosomal Impairment and ER Stress Activation for Boosted Autophagy Dysfunction Based on Te Double-Headed Nano-Bullets
Published: 2022-05-29
Journal: Small
Application: WB
Impact Factor: 15.15
Species: Mouse
PMID: 35644863
Estrogen protects against acidosis-mediated articular chondrocyte injury by promoting ASIC1a protein degradation
Published: 2021-07-24
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.43
Species: Rat
PMID: 34310912
A pH probe inhibits senescence in mesenchymal stem cells
Published: 2018-12-07
Journal: Stem Cell Research & Therapy
Application: WB
Impact Factor: 4.96
Species: Rat
PMID: 30526663
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PA1822
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 30, 2026