Anti-C-terminal-binding protein 1 CtBP1 Antibody
Rabbit IgG polyclonal antibody for C-terminal-binding protein 1(CTBP1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Anti-C-terminal-binding protein 1 CtBP1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-C-terminal-binding protein 1 CtBP1 Antibody
See all CTBP1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CTBP1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CTBP1 information: Molecular Weight: 47535 MW; Subcellular Localization: Cytoplasm . Nucleus ; Tissue Specificity: Expressed in germinal center B-cells.|
|Cite This Product||Anti-C-terminal-binding protein 1 CtBP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1570)|
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|Anti-C-terminal-binding protein 1 CtBP1 Antibody may replace the following items: sc 5961|sc 376004|sc 5963|sc 398945.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human CtBP1(425-440aa QTVKPEADRDHASDQL), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CTBP1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||1487|
|Species Of This Entry||Human|
|Protein Name||C-terminal-binding protein 1|
|Superfamily||D-isomer specific 2-hydroxyacid dehydrogenase family|
|Alternative Names||CTBP1|BARS; brefeldin A-ribosylated substrate; CTBP; CtBP1; C-terminal binding protein 1; C-terminal-binding protein 1; EC 1.1.1; EC 1.1.1.-; EC 220.127.116.11; MGC104684|
|Post Tranlational Modifications||Ribosylation; Acetylation; Phosphorylation; Sumoylation; Methylation; Deacetylation; Acylation; Demethylation; Reduction; Dephosphorylation; Ubiquitination|
|Subcellular Localization||Cytoplasm. Nucleus.|
|Gene Location||4p16.3, on Chromasome 4, gene sequence: NC_000004.12 (1211444..1250355, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CTBP1 (Source: Uniprot.org, NCBI)
|Protein Function||Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.|
|Tissue Specificity||Expressed in germinal center B-cells.|
|Interacting Proteins||BCAS3; CBX4; CDKN2D; CEP68; CRY2; CTBP2; FOXP2; HEMGN; HIC1; HOXB5; IKZF1; KLF12; KLF3; KLF4; MAPK9; NOL4L; NRIP1; PLCB1; PRKAA1; TERF2IP; TGIF1; THAP11; TSHZ3; ZNF366|
|Research Areas||Cell Cycle and Replication; Wnt Signaling Pathway|
|Related Diseases||Malignant Neoplasms; Neoplasms; Cell Transformation, Neoplastic; Malignant Neoplasm Of Breast; Neoplasm Metastasis; Carcinoma; Mammary Neoplasms; Leukemia; Malignant Tumor Of Colon; Cell Invasion; Melanoma; Neoplasm Invasiveness; Myeloid Leukemia; Adenomatous Polyposis Coli; Tumor Progression; Colonic Neoplasms; Colorectal Cancer; Adenoma; Malignant Paraganglionic Neoplasm|
|Related Pathways||Localization; Cell Cycle; Membrane Fission; Cell Proliferation; Chromatin Remodeling; Oncogenesis; Transport; Mitosis; Cell Migration; Macropinocytosis; Cell Differentiation; Dna Repair; Methylation; Rna Interference; Cell Adhesion; Histone Modification; Histone Acetylation; Secretory Pathway; Protein Transport; Nuclear Import|
|Background||CTBP1, C-terminal-binding protein 1, is a protein that in humans is encoded by the CTBP1 gene. The CtBP1 protein binds to the C-terminus of adenovirus E1A proteins. This gene is mapped to 4p16. This phosphoprotein is a transcriptional repressor(corepressor) and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. CtBP1 and CtBP2 preferentially associate with the E1A via a 5 amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of delta EF1, a cellular zinc finger-homeodomain protein, and thereby enhances delta EF1-induced transcriptional silencing. In addition, CtBP complexes with CtIP, a 125 kDa protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.|
|Scientific References||PMID: 7479821 by Schaeper U., et al. Molecular cloning and characterization of a cellular phosphoprotein that interacts with a conserved C-terminal domain of adenovirus E1A involved in negative modulation of oncogenic transformation.|
PMID: 9858600 by Sewalt R.G.A.B., et al. C-terminal binding protein is a transcriptional repressor that interacts with a specific class of vertebrate polycomb proteins.
Our Boster Quality Guarantee for Anti-C-terminal-binding protein 1 CtBP1 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: Rat Ovary Tissue Lysate
Lane 4: U87 Cell Lysate
Lane 5: SW620 Cell Lysate
Lane 6: HT1080 Cell Lysate
Lane 7: COLO32 Cell Lysate
IHC(P): Rat Brain Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Note: sample size takes longer to process, average 5-7 days.
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