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Product Info Summary
| SKU: | A03270-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PARP-2 Antibody
SKU/Catalog Number
A03270-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PARP-2 Antibody catalog # A03270-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PARP-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03270-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PARP2. AA range:151-200
Reactive Species
A03270-1 is reactive to PARP2 in Human, Mouse
Observed Molecular Weight
70 kDa
Calculated molecular weight
66.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03270-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of K562 cells using PARP-2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Specific Publications For Anti-PARP-2 Antibody (A03270-1)
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Customer Q&As
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4 Customer Q&As for Anti-PARP-2 Antibody
Question
We are currently using anti-PARP-2 antibody A03270-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
The anti-PARP-2 antibody (A03270-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-04
Question
Can you help my question with product A03270-1, anti-PARP-2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Evans
Verified customer
Asked: 2019-08-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03270-1 anti-PARP-2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-20
Question
Would anti-PARP-2 antibody A03270-1 work on canine WB with fetal brain?
J. Krishna
Verified customer
Asked: 2019-02-15
Answer
Our lab technicians have not tested anti-PARP-2 antibody A03270-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PARP-2 antibody A03270-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine fetal brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-15
Question
I was wanting to use your anti-PARP-2 antibody for WB for mouse fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse fetal brain identification?
G. Li
Verified customer
Asked: 2016-12-22
Answer
As indicated on the product datasheet, A03270-1 anti-PARP-2 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-12-22
