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Product Info Summary
| SKU: | M01288-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Pax2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01288-1
BM4096 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Pax2 Rabbit Monoclonal Antibody catalog # M01288-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pax2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01288-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BDA-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Pax2
Reactive Species
M01288-1 is reactive to PAX2 in Human, Mouse
Observed Molecular Weight
45 kDa
Calculated molecular weight
44.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01288-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
FC 1:20
Positive Control
WB: Human fetal kidney
IHC: human colon tissue
Validation Images & Assay Conditions
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Western blot analysis of Pax2 expression in Human fetal kidney lysate.
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Immunohistochemical analysis of paraffin-embedded human colon using Pax2 Antibody.
Specific Publications For Anti-Pax2 Rabbit Monoclonal Antibody (M01288-1)
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4 Customer Q&As for Anti-Pax2 Rabbit Monoclonal Antibody
Question
We are currently using anti-Pax2 Rabbit Monoclonal antibody M01288-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
O. Walker
Verified customer
Asked: 2019-12-03
Answer
The anti-Pax2 Rabbit Monoclonal antibody (M01288-1) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-03
Question
We were well pleased with the WB result of your anti-Pax2 Rabbit Monoclonal antibody. However we have observed positive staining in cortex of kidney nucleus. using this antibody. Is that expected? Could you tell me where is PAX2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
Based on literature, cortex of kidney does express PAX2. Generally PAX2 expresses in nucleus. Regarding which tissues have PAX2 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 1378753
Kidney cortex, Pubmed ID: 7819127, 8661132
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2019-03-22
Question
We purchased anti-Pax2 Rabbit Monoclonal antibody for WB on kidney last year. I am using mouse, and We intend to use the antibody for IHC next. My lab would like examining kidney as well as kidney cortex in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
T. Edwards
Verified customer
Asked: 2018-12-05
Answer
I looked at the website and datasheets of our anti-Pax2 Rabbit Monoclonal antibody and it appears that M01288-1 has been validated on mouse in both WB and IHC. Thus M01288-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-05
Question
We have been able to see staining in mouse cortex of kidney. Are there any suggestions? Is anti-Pax2 Rabbit Monoclonal antibody supposed to stain cortex of kidney positively?
K. Evans
Verified customer
Asked: 2013-07-23
Answer
From what I have seen in literature cortex of kidney does express PAX2. From what I have seen in Uniprot.org, PAX2 is expressed in cortex of kidney, kidney, kidney cortex, leukemic t-cell, among other tissues. Regarding which tissues have PAX2 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 1378753
Kidney cortex, Pubmed ID: 7819127, 8661132
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2013-07-23
