Product Info Summary
SKU: | A00943-4 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-PAX8 Antibody Picoband™
SKU/Catalog Number
A00943-4
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PAX8 Antibody Picoband™ catalog # A00943-4. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PAX8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00943-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human PAX8 recombinant protein (Position: Q137-L450). Human PAX8 shares 95.3% and 95% amino acid (aa) sequence identity with mouse and rat PAX8, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00943-4 is reactive to PAX8 in Human, Mouse, Rat
Applications
A00943-4 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
48 kDa
Calculated molecular weight
48.218kDa
Background of PAX8
Paired box gene 8, also known as PAX8, is a protein which in humans is encoded by the PAX8 gene. This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PAX8 using anti-PAX8 antibody (A00943-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SW579 whole cell lysates,
Lane 2: human SK-OV-3 whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat NRK whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX8 antigen affinity purified polyclonal antibody (Catalog # A00943-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX8 at approximately 48 kDa. The expected band size for PAX8 is at 48 kDa.
Click image to see more details
Figure 2. IF analysis of PAX8 using anti-PAX8 antibody (A00943-4) and anti-Beta Tubulin antibody (M01857-3).
PAX8 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PAX8 Antibody (A00943-4) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For PAX8 (Source: Uniprot.org, NCBI)
Gene Name
PAX8
Full Name
Paired box protein Pax-8
Weight
48.218kDa
Alternative Names
paired box 8; paired box gene 8; paired box protein Pax-8; paired domain gene 8 PAX8 paired box 8 paired box protein Pax-8|paired domain gene 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAX8, check out the PAX8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAX8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAX8 Antibody Picoband™ (A00943-4)
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No publications found for A00943-4
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