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Product Info Summary
| SKU: | M01153 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PBR TSPO Rabbit Monoclonal Antibody
SKU/Catalog Number
M01153
BM4275 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PBR TSPO Rabbit Monoclonal Antibody catalog # M01153. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PBR TSPO Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01153)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DAG-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PBR
Reactive Species
M01153 is reactive to TSPO in Human, Mouse
Observed Molecular Weight
16 kDa
Calculated molecular weight
18.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01153 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
IHC: human colon tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colon, using PBR Antibody .
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
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Western blot analysis of Calreticulin expression in (1) U87-MG cell lysate; (2) A431 cell lysate.
Specific Publications For Anti-PBR TSPO Rabbit Monoclonal Antibody (M01153)
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-PBR TSPO Rabbit Monoclonal Antibody
Question
Can M01153 target dTSPO?
Verified customer
Asked: 2019-05-09
Answer
We haven't detected dTSPO using Anti-PBR TSPO Rabbit Monoclonal Antibody (M01153). The immunogen sequence is CRDNHGWHGGRRLPE.
Boster Scientific Support
Answered: 2019-05-10
Question
We are currently using anti-PBR Rabbit Monoclonal antibody M01153 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
The anti-PBR Rabbit Monoclonal antibody (M01153) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-26
