Anti-Apolipoprotein A II APOA2 Monoclonal Antibody

Apolipoprotein A-II/ApoA2 antibody

Boster Bio Anti-Apolipoprotein A II APOA2 Monoclonal Antibody catalog # M02088. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: M02088
Size: 100 μl
Reactive Species: Human, Rat
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-Apolipoprotein A II APOA2 Monoclonal Antibody

View all Apolipoprotein A-II/ApoA2 Antibodies

SKU/Catalog Number

M02088

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Apolipoprotein A II APOA2 Monoclonal Antibody catalog # M02088. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Apolipoprotein A II APOA2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02088)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEIA-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Apolipoprotein A II May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M02088 is reactive to APOA2 in Human, Rat

Applications

M02088 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

11.175kDa

Background of Apolipoprotein A-II/ApoA2

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For APOA2 (Source: Uniprot.org, NCBI)

Gene Name

APOA2

Full Name

Apolipoprotein A-II

Weight

11.175kDa

Superfamily

apolipoprotein A2 family

Alternative Names

Alp-2; APOA2; apoAII; Apo-AII; ApoA-II; Apolipoprotein A2; Apolipoprotein AII; Apolipoprotein A-II; Hdl-1 APOA2 Apo-AII, ApoA-II, apoAII apolipoprotein A2 apolipoprotein A-II

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on APOA2, check out the APOA2 Infographic

APOA2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for APOA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Apolipoprotein A II APOA2 Monoclonal Antibody

Question

I see that the anti-Apolipoprotein A II Monoclonal antibody M02088 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-17

Question

Would anti-Apolipoprotein A II Monoclonal antibody M02088 work on feline WB with liver?

Verified Customer

Verified customer

Asked: 2019-11-07

Answer

Our lab technicians have not validated anti-Apolipoprotein A II Monoclonal antibody M02088 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Apolipoprotein A II Monoclonal antibody M02088 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-07

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-Apolipoprotein A II Monoclonal antibody M02088. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-07

Question

Is a blocking peptide available for product anti-Apolipoprotein A II Monoclonal antibody (M02088)?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

We do provide the blocking peptide for product anti-Apolipoprotein A II Monoclonal antibody (M02088). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-18

Question

Would anti-Apolipoprotein A II Monoclonal antibody M02088 work for IF with plasma?

C. Johnson

Verified customer

Asked: 2016-01-06

Answer

According to the expression profile of plasma, APOA2 is highly expressed in plasma. So, it is likely that anti-Apolipoprotein A II Monoclonal antibody M02088 will work for IF with plasma.

Boster Scientific Support

Answered: 2016-01-06

Question

We are currently using anti-Apolipoprotein A II Monoclonal antibody M02088 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on zebrafish tissues as well?

E. Miller

Verified customer

Asked: 2013-06-24

Answer

The anti-Apolipoprotein A II Monoclonal antibody (M02088) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-06-24

Order DetailsPrice
M02088

100μL

$370
M02088-30ul

30μL sample

$99
M02088-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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