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Product Info Summary
| SKU: | M02088 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Apolipoprotein A II APOA2 Monoclonal Antibody
SKU/Catalog Number
M02088
BM5624 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Apolipoprotein A II APOA2 Monoclonal Antibody catalog # M02088. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apolipoprotein A II APOA2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02088)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEIA-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Apolipoprotein A II May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
Reactive Species
M02088 is reactive to APOA2 in Human, Rat
Observed Molecular Weight
11 kDa
Calculated molecular weight
11.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02088 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: Human plasma
Validation Images & Assay Conditions
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Western blot analysis of Apolipoprotein A II expression in Human plasma lysate.
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Expression analyses of transcription or protein levels of selected genes. (A) Expression analyses of selected genes by qRT–PCR. The data represent the means ± SDs from six biological replicates with three technical replicates. (B–D) FCJ/RCJ upregulated ABCC3, IDI1, and APOA2 expression in the liver. FCJ and RCJ significantly upregulated hepatic ABCC3 (B) and IDI1 (C) expression, and RCJ significantly upregulated hepatic APOA2 (D) expression in T2DM rats. The data represent the means ± SDs from three biological replicates with three technical replicates. * p < 0.05, *** p < 0.001, **** p < 0.0001.
Index in PubMed under a CC BY license. PMID: 40458826
Specific Publications For Anti-Apolipoprotein A II APOA2 Monoclonal Antibody (M02088)
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Customer Q&As
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6 Customer Q&As for Anti-Apolipoprotein A II APOA2 Monoclonal Antibody
Question
I see that the anti-Apolipoprotein A II Monoclonal antibody M02088 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-17
Question
Would anti-Apolipoprotein A II Monoclonal antibody M02088 work on feline WB with liver?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
Our lab technicians have not validated anti-Apolipoprotein A II Monoclonal antibody M02088 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Apolipoprotein A II Monoclonal antibody M02088 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-Apolipoprotein A II Monoclonal antibody M02088. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-07
Question
Is a blocking peptide available for product anti-Apolipoprotein A II Monoclonal antibody (M02088)?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We do provide the blocking peptide for product anti-Apolipoprotein A II Monoclonal antibody (M02088). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-18
Question
Would anti-Apolipoprotein A II Monoclonal antibody M02088 work for IF with plasma?
C. Johnson
Verified customer
Asked: 2016-01-06
Answer
According to the expression profile of plasma, APOA2 is highly expressed in plasma. So, it is likely that anti-Apolipoprotein A II Monoclonal antibody M02088 will work for IF with plasma.
Boster Scientific Support
Answered: 2016-01-06
Question
We are currently using anti-Apolipoprotein A II Monoclonal antibody M02088 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on zebrafish tissues as well?
E. Miller
Verified customer
Asked: 2013-06-24
Answer
The anti-Apolipoprotein A II Monoclonal antibody (M02088) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-24
