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Product Info Summary
| SKU: | A09172 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TOM70 TOMM70 Antibody
SKU/Catalog Number
A09172
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TOM70 TOMM70 Antibody (Catalog # A09172). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TOM70 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TOM70 TOMM70 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09172)
Host
Rabbit
Contents
TOM70 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-TOM70 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human TOM70. The immunogen is located within amino acids 50-100 of TOM70.
Cross-reactivity
At least two isoforms of TOM70 are known to exist; this antibody will only detect the larger isoform.
Reactive Species
A09172 is reactive to TOMM70A in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
67.5 kDa
Background of TOMM70A
The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria. TOM70, an important member of the TOM complex, contains a tetratricopeptide repeat domain similar to those found in cytosolic chaperones such as Hsp90 and Hsc70 and provides a docking site for these proteins. This interaction is thought to be a critical first step in the TOM70-dependent mitochondrial import, followed by contact between the preprotein and TOM70. After targeting to TOM70, preproteins are translocated through the outer membrane via the TOM40 import pore complex. The precise mechanism of how preproteins progress from TOM70 to TOM40 to full translocation is still unclear.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09172 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-4 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: TOM70 A09172 (2 μg/mL), TOM70 4959 (2 μg/mL) and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human and Mouse Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: TOM70 A09172 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Mouse Brain
Loading: 15 μg of lysates per lane.
Antibodies: TOM70 A09172 (Lane 1: 2 μg/mL and Lane 2: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Rat Brain
Loading: 15 μg of lysates per lane.
Antibodies: TOM70 A09172 (Lane 1: 2 μg/mL and Lane 2: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of TOM70 in Mouse Brain Tissue
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TOM70 antibody (A09172) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of TOM70 in Mouse Brain Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TOM70 with A09172 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Specific Publications For Anti-TOM70 TOMM70 Antibody (A09172)
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