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Product Info Summary
| SKU: | M09495-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-PCDH7 Mouse Monoclonal Antibody [Clone ID: OTI2G6]
SKU/Catalog Number
M09495-1
Size
100 µl
Description
Boster Bio PCDH7 mouse monoclonal antibody, clone OTI2G6 (formerly 2G6). Catalog# M09495-1. Tested in IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PCDH7 Mouse Monoclonal Antibody [Clone ID: OTI2G6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09495-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2G6
Isotype
IgG1
Immunogen
Full length human recombinant protein of human PCDH7 (NP_115833) produced in HKE293T cell.
Reactive Species
M09495-1 is reactive to PCDH7 in Human, Mouse, Rat
Calculated molecular weight
116.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09495-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:200~2000
IHC 1:150
IF 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCDH7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCDH7.
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Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-PCDH7 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PCDH7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PCDH7 monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PCDH7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Anti-PCDH7 mouse monoclonal antibody (M09495-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PCDH7.
Specific Publications For Anti-PCDH7 Mouse Monoclonal Antibody [Clone ID: OTI2G6] (M09495-1)
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