Anti-PCNA Antibody (Monoclonal, PC 10)

Boster Bio Anti-PCNA Antibody (Monoclonal, PC 10) catalog # MA1083. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 131 publication(s).

Product Info Summary

SKU: MA1083
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: IHC, ICC, WB

Product Name

Anti-PCNA Antibody (Monoclonal, PC 10)

See all Pcna products

SKU/Catalog Number

MA1083

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PCNA Antibody (Monoclonal, PC 10) catalog # MA1083. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PCNA Antibody (Monoclonal, PC 10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1083)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

PC 10

Isotype

Mouse IgG2a

Immunogen

Protein A fusion protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1083 is reactive to Pcna in Human, Mouse, Rat

Applications

MA1083 is guaranteed for IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, mouse, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry (Frozen Section), 0.4-1μg/ml, Human, mouse, rat, -
Western blot, 2μg/ml, Human, mouse, rat

Validation Images & Assay Conditions

Gene/Protein Information For Pcna (Source: Uniprot.Org, NCBI)

Uniprot ID

P04961

Gene ID

25737

Gene Name

Pcna

Full Name

Proliferating cell nuclear antigen

Weight

28.749kDa

Superfamily

PCNA family

Alternative Names

cyclin; DNA polymerase delta auxiliary protein; MGC8367; proliferating cell nuclear antigen Pcna|PCNAR/cyclin, Pcna|proliferating cell nuclear antigen|proliferating cell nuclear antigen|cyclin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Pcna, check out the Pcna Infographic

Pcna infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Pcna: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1083 has been cited in 131 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ma Q,Gu JT,Wang B,Feng J,Yang L,Kang XW,Duan P,Sun X,Liu PJ,Wang JC.PlGF signaling and macrophage repolarization contribute to the anti-neoplastic effect of metformin.Eur J Pharmacol.2019 Nov 15;863:172696.doi:10.1016/j.ejphar.2019.172696.Epub 2019 Sep 25.PMID:31562866.
Species: Human,Mouse
MA1083 usage in article: APP:IHC, SAMPLE:BREAST TISSUE, DILUTION:NA

Ge Y,Cheng R,Sun S,Zhang S,Li L,Jiang J,Yang C,Xuan X,Chen J.Fangxiao Formula alleviates airway inflammation and remodeling in rats with asthma via suppression of transforming growth factor -β/Smad3 signaling pathway.Biomed Pharmacother.2019 Nov;119:109429.doi:10.1016/j.biopha.2019.109429.Epub 2019 Sep 7.PMID:31505422.
Species: Rat
MA1083 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:NA

Xiong W,Wu L,Tang R,Zhang Q,Guo Q,Song S.Grape Seed Proanthocyanidins (GSPs) Inhibit the Development of Cutaneous Squamous Cell Carcinoma by Regulating the hsa_circ_0070934/miR-136-5p/PRAF2 Axis.Cancer Manag Res.2021;13:4359-4371https://doi. org/10.2147/CMAR.S302084
Species: Human,Mouse
MA1083 usage in article: APP:WB, SAMPLE:A431 CELL AND SCL-1 CELL, DILUTION:1:2000

Tan PC,Chao PC,Cheng C,Chen CH,Huang RL,Zhou SB,Xie Y,Li QF.A randomized, controlled clinical trial of autologous stromal vascular fraction cells transplantation to promote mechanical stretch-induced skin regeneration.Stem Cell Res Ther.2021 Apr 15;12(1):243.doi:10.1186/s13287-021-02318-5.PMID:33858504;PMCID:P MC8048343.
Species: Human
MA1083 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:1:100

Fan R,Wang H,Zhang L,Ma T,Tian Y,Li H.Nanocrystallized Oleanolic Acid Better Inhibits Proliferation, Migration and Invasion in Intracranial Glioma via Caspase-3 Pathway.J Cancer.2020 Jan 29;11(7):1949-1958.doi:10.7150/jca. 38847.PMID:32194806;PMCID:PMC7052863.
Species: Human,Mouse
MA1083 usage in article: APP:IHC, SAMPLE:GLIOMA TISSUE, DILUTION:NA

Yi M,Zhang J,Li A,Niu M,Yan Y,Jiao Y,Luo S,Zhou P,Wu K.The construction, expression, and enhanced anti-tumor activity of YM101: a bispecific antibody simultaneously targeting TGF-β and PD-L1.J Hematol Oncol.2021 Feb 16;14(1):27.doi:10.1186/s13045-021-0104
Species: Human,Mouse

Zhang F,Zhang L,He L,Cao M,Yang Y,Duan X,Shi J,Liu K.A PITX2 splice-site mutation in a family with Axenfeld-Rieger syndrome leads to decreased expression of nuclear PITX2 protein.Int Ophthalmol.2021 Jan 25.doi:10.1007/s10792-021-01704-5.Epub ahead of prin
Species: Human
MA1083 usage in article: APP:WB, SAMPLE:LYMPHOCYTES, DILUTION:NA

Yiwei Shi,Xiaojiang Qin,Xiaomin Hou et al.Overexpression of Krüppel-Like Factor 4 (KLF4) Inhibits the Proliferation of Pulmonary Arterial Smooth Muscle Cells Through ERK1/2 Signaling Under Hypoxia Environment,18 January 2021,PREPRINT (Version 1) available
Species: Rat
MA1083 usage in article: APP:WB, SAMPLE:PASMCS, DILUTION:1:200

Nicotinamide Supplementation Attenuates Renal Interstitial Fibrosis via Boosting the Activity of Sirtuins. Xin Zhen,Shaowu Zhang,Feifei Xie,Miaomiao Zhou,Zheng Hu,Fengxin Zhu,Jing Nie
Species: Mouse
MA1083 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE AND PTCS, DILUTION:NA

Xie,J.,Liu,W.,Lv,W.,Han,X.,Kong,Q.,Wu,Y.,Liu,X.,Han,Y.,Shi,C.,& Jia,X.(2020).Transmembrane protein 16A/anoctamin 1 inhibitor T16Ainh-A01 reversed monocrotaline-induced rat pulmonary arterial hypertension. Pulmonary Circulation. https://doi.org/10.1177/204
Species: Rat
MA1083 usage in article: APP:WB, SAMPLE:PA TISSUE, DILUTION:1:250

Guo,Hongrui & Li,Xinyang & Li,Xiaodan & Jiang, Xinyue & Lai,Weiming & Geng,Yi & Ouyang,Ping & He,Ran & Tang,Huaqiao & Xie,Yue & Yang,Guang-You & Gu,Xiaobin.(2020).Immunologic Improvement of Vaccine for Newcastle Disease via Co-Inoculation of Cattle Transf
Species: Broiler
MA1083 usage in article: APP:IHC, SAMPLE:SPLEEN,THYMUS AND BURSA OF FABRICIUS, DILUTION:1:100

Zhou,W.,Xi,D.,Shi,Y.,Wang,L.,Zhong,H.,Huang,Z. ... He, F.(2021).MicroRNA‑1929‑3p participates in murine cytomegalovirus‑induced hypertensive vascular remodeling through Ednra/NLRP3 inflammasome activation. International Journal of Molecular Medicine,47,71
Species: Mouse
MA1083 usage in article: APP:WB, SAMPLE:AORTIC TISSUE, DILUTION:1:250

Study of the correlation between the expression of nuclear factor kappa B and proliferation regulatory proteins and chronic superficial gastritis,Hu Hui,Ma Zhijian,Ren Shouzhong,Xie Yiqiang, Vojnosanitetski pregled 2020 OnLine-First,00,SP - 135 EP-135
Species: Rat
MA1083 usage in article: APP:IHC, SAMPLE:STOMACH TISSUE, DILUTION:NA

Zhang H,Liu S,Cai Z,Dong W,Ye J,Cai Z,Han Z,Liang Y,Zhuo Y,Luo Y,Zhu X,Deng Y,Zhang Y,Liu R,Feng Y, Lai J,Zhou R,Tan H,Zhong W. Down-regulation of ACACA suppresses the malignant progression of Prostate Cancer through inhibiting mitochondrial potential. J
Species: Human
MA1083 usage in article: APP:IHC, SAMPLE:REGIONAL LYMPH NODE AND PROSTATE TISSUE, DILUTION:1:100

Yu Y,Wang Y,Fei X,Song Z,Xie F,Yang F,Liu X,Xu Z,Wang G.All-Trans Retinoic Acid Prevented Vein Grafts Stenosis by Inhibiting Rb-E2F Mediated Cell Cycle Progression and KLF5-RARα Interaction in Human Vein Smooth Muscle Cells.Cardiovasc Drugs Ther.2020 Oct
Species: Human,New Zealand White Rabbit
MA1083 usage in article: APP:WB, SAMPLE:HUVSMCS, DILUTION:1:500

Zhou C,He X,Zeng Q,Zhang P,Wang CT.CCDC7 Activates Interleukin-6 and Vascular Endothelial Growth Factor to Promote Proliferation via the JAK-STAT3 Pathway in Cervical Cancer Cells.Onco Targets Ther.2020 Jun 30;13:6229-6244.doi:10.2147/OTT.S244663.PMID:326
Species: Human,Mouse
MA1083 usage in article: APP:WB, SAMPLE:HELA CELL, DILUTION:NA

Zhao Y,Tang N,Xi D,Huang Z,Zhang T,Liu Y,Wang L,Tang Y,Zhong H,He F.Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin-angiotensin system.Exp Ther Med
Species: Rat
MA1083 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:1:250

Turan G,Turan M.The Evaluation of TUNEL, PCNA and SOX2 Expressions in Lens Epithelial Cells of Cataract Patients with Pseudoexfoliation Syndrome.Curr Eye Res.2020 Jan;45(1):12-16.doi:10.1080/02713683.2019. 1657463.Epub 2019 Aug 27.Erratum in:Curr Eye Res.
Species: Human
MA1083 usage in article: APP:IHC, SAMPLE:ANTERIOR CAPSULE, DILUTION:1:100

Wang S,Zhang C,Zhang X.Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells.Mol Med Rep.2020 Feb;21(2):589-596.doi:10.3892/mmr.2019.10887.Epub 2019 Dec 17.PMID:31974617; PMC
Species: Human
MA1083 usage in article: APP:WB, SAMPLE:HPASMCS, DILUTION:1:400

Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
Species: Human
MA1083 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION:NA

Wei Y, Yang D, Du X, Yu X, Zhang M, Tang F, Ma F, Li N, Bai C, Li G, Hua J. Interaction between DMRT1 and PLZF protein regulates self-renewal and proliferation in male germline stem cells. Mol Cell Biochem. 2020 Nov 16. doi: 10.1007/s11010-020-03977-3. Ep
Species: Human,Lentivirus,Mouse
MA1083 usage in article: APP:IF, SAMPLE:MALE GERMLINE STEM CELLS, DILUTION:1:200

Deng Y,Guo SL,Li JQ,Xie SS,Zhou YC,Wei B,Wang Q,Wang F. Downregulation of Interferon regulatory factor 7 is link to pulmonary vascular smooth muscle cell proliferation and inflammation in Monocrotaline-induced pulmonary hypertension in rats. Life Sci.2020
Species: Rat

Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin%u2011angiotensin system %u2026

Up-regulation of multiple proteins and biological processes during maxillary expansion in rats

The enhanced antitumor-specific immune response with mannose-and CpG-ODN-coated liposomes delivering TRP2 peptide

Enhanced inhibitive effects of combination of rofecoxib and octreotide on the growth of human gastric cancer

A model for implantation: coculture of blastocysts and uterine endometrium in mice

Caveolin%u20111 facilitated KCNA5 expression, promoting breast cancer viability

Collagen I enhances the efficiency and anti-tumor activity of dendritic-tumor fusion cells

Gga-miR-21 inhibits chicken pre-adipocyte proliferation in part by down-regulating Kruppel-like factor 5

Reduction of G0 phase cells of colon cancer caco-2 cells may enhance 5-fluorouracil efficacy

Synergistic inhibitory effects of capsaicin combined with cisplatin on human osteosarcoma in culture and in xenografts

Circadian locomotor output cycles kaput affects the proliferation and migration of breast cancer cells by regulating the expression of E-cadherin via IQ motif containing GTPase activating protein 1

Extracorporeal shock wave therapy with low-energy flux density inhibits hypertrophic scar formation in an animal model

Aberrant rhythmic expression of cryptochrome2 regulates the radiosensitivity of rat gliomas

Effects of 4-nonylphenol on oxidant/antioxidant balance system inducing hepatic steatosis in male rat

Nucleocapsid Interacts with NPM1 and Protects it from Proteolytic Cleavage, Enhancing Cell Survival, and is Involved in PEDV Growth

Folate-modified Chitosan Nanoparticles Containing the IP-10 Gene Enhance Melanoma-specific Cytotoxic CD8(+)CD28(+) T Lymphocyte Responses

Comparison of the synergistic effect of lipid nanobubbles and SonoVue microbubbles for high intensity focused ultrasound thermal ablation of tumors

Involvement of MicroRNA-210 Demethylation in Steroid-associated Osteonecrosis of the Femoral Head

Effect of P2X4R on airway inflammation and airway remodeling in allergic airway challenge in mice

Absence of the Adenosine A2A Receptor Confers Pulmonary Arterial Hypertension and Increased Pulmonary Vascular Remodeling in Mice

Circadian genesPer1andPer2increase radiosensitivity of gliomain

Inhibition of Lymphangiogenesis of Endothelial Progenitor Cells with VEGFR-3 siRNA Delivered with PEI-alginate Nanoparticles

Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase

Expression of gonadotropin-releasing hormone receptor and effect of gonadotropin-releasing hormone analogue on proliferation of cultured gastric smooth muscle cells of rats

Developmental changes in intercellular junctions and Kv channels in the intestine of piglets during the suckling and post-weaning periods

Exosomes from Human Umbilical Cord Mesenchymal Stem Cells: Identification, Purification, and Biological Characteristics

Endothelial lipase is upregulated by interleukin-6 partly via the p38 MAPK and p65 NF-?B signaling pathways

Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension

Effects of tripterygium glycosides on restenosis following endovascular treatment

Improved prescription of taohechengqi-tang alleviates D-galactosamine acute liver failure in rats

Dietary NiCl2 causes G2/M cell cycle arrest in the broiler's kidney

Developmental changes in cell proliferation and apoptosis in the normal duck bursa of Fabricius

Peng L, Xie YF, Wang CG, Wu HG, Liu M, Wang YD, Ma FQ, Chang XR, Yang ZB. Afr J Tradit Complement Altern Med. 2017 Jan 13;14(2):148-160. doi: 10.21010/ajtcam.v14i2.16. eCollection 2017. MOXIBUSTION ALLEVIATES GASTRIC PRECANCEROUS LESIONS IN RATS B...

Fanglin Ma, Zhe Zhou, Na Li, Liming Zheng, Chongyang Wu, Bowen Niu, Furong Tang, Xin He, Guangpeng Li & Jinlian Hua Scientific Reports 6, Article number: 38805 (2016) doi:10.1038/srep38805 Lin28a promotes self-renewal and proliferation of dairy...

Baisong Zhao, Yongying Pan, Zixin Wang, Haiping Xu and Xingrong Song. Yonsei Med J. 2017 Jan;58(1):131-138. Hyperbaric Oxygen Pretreatment Improves Cognition and Reduces Hippocampal Damage Via p38 Mitogen-Activated Protein Kinase in a Rat Model

Qiu M, Bao W, Wang J, Yang T, He X, Liao Y, Wan X. Bmc Cancer. 2014 Feb 11;14:78. Doi: 10.1186/1471-2407-14-78. Foxa1 Promotes Tumor Cell Proliferation Through Ar Involving The Notch Pathway In Endometrial Cancer.

Bao W, Fan Q, Luo X, Cheng Ww, Wang Yd, Li Zn, Chen Xl, Wu D. Oncol Rep. 2013 Aug;30(2):723-30. Doi: 10.3892/Or.2013.2496. Epub 2013 May 27. Silencing Of Cathepsin B Suppresses The Proliferation And Invasion Of Endometrial Cancer.

Chen G, Liang Yx, Zhu Jg, Fu X, Chen Yf, Mo Rj, Zhou L, Fu H, Bi Xc, He Hc, Yang Sb, Wu Yd, Jiang Fn, Zhong Wd. Biomed Res Int. 2014;2014:230183. Doi: 10.1155/2014/230183. Epub 2014 Aug 17. Cc Chemokine Ligand 18 Correlates With Malignant Progress...

Xiang S, Liu J, Dong N, Shi J, Xiao Y, Wang Y, Hu X, Gong L, Wang W. J Vasc Res. 2014;51(2):132-43. Doi: 10.1159/000355193. Epub 2014 Mar 28. Suppressor Of Cytokine Signaling 3 Is A Negative Regulator For Neointimal Hyperplasia Of Vein Graft Steno...

Wu L, Zhang W, Tang Yh, Li H, Chen By, Zhang Gm, Deng Cq. Phytomedicine. 2010 Mar;17(3-4):233-40. Doi: 10.1016/J.Phymed.2009.07.021. Epub 2009 Sep 11. Effect Of Total Saponins Of "Panax Notoginseng Root" On Aortic Intimal Hyperplasia And The Expre...

Xu T, Ni Mm, Huang C, Meng Xm, He Yh, Zhang L, Li J. Inflammation. 2015 Oct;38(5):1794-804. Doi: 10.1007/S10753-015-0157-6. Nlrc5 Mediates Il-6 And Il-1?? Secretion In Lx-2 Cells And Modulated By The Nf-??b/Smad3 Pathway.

Chen Y, Qian H, Zhu W, Zhang X, Yan Y, Ye S, Peng X, Li W, Xu W. Stem Cells Dev. 2011 Jan;20(1):103-13. Doi: 10.1089/Scd.2009.0495. Epub 2010 Oct 12. Hepatocyte Growth Factor Modification Promotes The Amelioration Effects Of Human Umbilical Cord M...

Zhang X, Shi K, Li Y, Zhang H, Hao J. Reprod Toxicol. 2014 Jun;45:87-93. Doi: 10.1016/J.Reprotox.2014.01.009. Epub 2014 Feb 4. Lipopolysaccharide Inhibits The Self-Renewal Of Spermatogonial Stem Cells In Vitro Via Downregulation Of Gdnf Expression...

Chen Q, Zhang Z, Liu J, He Q, Zhou Y, Shao G, Sun X, Cao X, Gong A, Jiang P. Mol Cells. 2015 Mar;38(3):221-8. Doi: 10.14348/Molcells.2015.2170. Epub 2015 Feb 4. A Fibrin Matrix Promotes The Differentiation Of Emscs Isolated From Nasal Respiratory ...

Zhang C, Peng Y, Wang F, Tan X, Liu N, Fan S, Wang D, Zhang L, Liu D, Wang T, Wang S, Zhou Y, Su Y, Cheng T, Zhuang Z, Shi C. Biomaterials. 2010 Dec;31(36):9535-43. Doi: 10.1016/J.Biomaterials.2010.08.059. Epub 2010 Sep 26. A Synthetic Cantharidin...

Wang S, Yang S, Lai Z, Ding T, Shen W, Shi L, Jiang J, Ma L, Tian Y, Du X, Luo A, Wang S. Cell Tissue Res. 2013 Nov;354(2):609-21. Doi: 10.1007/S00441-013-1678-7. Epub 2013 Jul 4. Effects Of Culture And Transplantation On Follicle Activation And E...

Ma Hp, Ma Xn, Ge Bf, Zhen P, Zhou J, Gao Yh, Xian Cj, Chen Km. Cell Prolif. 2014 Dec;47(6):527-39. Doi: 10.1111/Cpr.12147. Epub 2014 Oct 30. Icariin Attenuates Hypoxia-Induced Oxidative Stress And Apoptosis In Osteoblasts And Preserves Their Osteo...

Fang J, Cui H, Peng X, Chen Z, He M, Tang L. Anat Histol Embryol. 2011 Dec;40(6):457-65. Doi: 10.1111/J.1439-0264.2011.01094.X. Epub 2011 Jun 15. Developmental Changes In Cell Proliferation And Apoptosis In The Normal Duck Thymus.

Liao J, Chen X, Li Y, Ge Z, Duan H, Zou Y, Ge J. J Biomed Biotechnol. 2012;2012:165296. Doi: 10.1155/2012/165296. Epub 2012 May 14. Transfer Of Bone-Marrow-Derived Mesenchymal Stem Cells Influences Vascular Remodeling And Calcification After Ballo...

Huang C, Wang X, Sun B, Li M, Zhao X, Gu Y, Cui Y, Li Y. Target Oncol. 2015 Mar;10(1):85-97. Doi: 10.1007/S11523-014-0316-Y. Epub 2014 May 17. Study On Mouse Model Of Triple-Negative Breast Cancer: Association Between Higher Parity And Triple-Nega...

Tan Tq, Ge C, Mi Y, Jin Y, Zhang C. Cell Biol Int. 2010 Jul;34(7):769-75. Doi: 10.1042/Cbi20090244. Ginsenosides Promote Proliferation Of Granulosa Cells From Chicken Prehierarchical Follicles Through Pkc Activation And Up-Regulated Cyclin Gene Ex...

Qiao Q, Teng X, Wang N, Lu R, Guo L, Zhang X, Du Y, Wang W, Chen S, Wu Q, He G, Wang Y, Hu W. Faseb J. 2014 Nov;28(11):4986-99. Doi: 10.1096/Fj.14-258046. Epub 2014 Aug 11. A Novel Crig-Targeted Complement Inhibitor Protects Cells From Complement ...

Chen Jl, Shang Qh, Hu W, Liu C, Mao Wh, Liu Hq. J Geriatr Cardiol. 2012 Jun;9(2):185-91. Doi: 10.3724/Sp.J.1263.2012.04011. Role Of Tgf-??1/Smads Pathway In Carotid Artery Remodeling In Renovascular Hypertensive Rats And Prevention By Enalapril An...

Wang H, Bao W, Jiang F, Che Q, Chen Z, Wang F, Tong H, Dai C, He X, Liao Y, Liu B, Sun J, Wan X. Cancer Lett. 2015 May 1;360(2):269-79. Doi: 10.1016/J.Canlet.2015.02.028. Epub 2015 Feb 16. Mutant P53 (P53-R248Q) Functions As An Oncogene In Promoti...

Sang J, Chen Y, Tao Y. Mol Med Rep. 2011 Nov-Dec;4(6):1163-7. Doi: 10.3892/Mmr.2011.535. Epub 2011 Jul 15. Nitric Oxide Inhibits Gastric Cancer Cell Growth Through The Modulation Of The Akt Pathway.

Zhou Ll, Hou Ff, Wang Gb, Yang F, Xie D, Wang Yp, Tian Jw. Kidney Int. 2009 Dec;76(11):1148-60. Doi: 10.1038/Ki.2009.322. Epub 2009 Sep 2. Accumulation Of Advanced Oxidation Protein Products Induces Podocyte Apoptosis And Deletion Through Nadph-De...

Lin Cm, Yuan Yp, Chen Xc, Li Hh, Cai Bz, Liu Y, Zhang H, Li Y, Huang K. J Mol Histol. 2015 Jun;46(3):233-40. Doi: 10.1007/S10735-015-9616-5. Epub 2015 Apr 2. Expression Of Wnt/??-Catenin Signaling, Stem-Cell Markers And Proliferating Cell Markers ...

Zhao Cl, Zhang Gp, Xiao Zz, Ma Zk, Lei Cp, Song Sy, Feng Yy, Zhao Yc, Feng Xs. J Breast Cancer. 2015 Jun;18(2):126-33. Doi: 10.4048/Jbc.2015.18.2.126. Epub 2015 Jun 26. Recombinant Human Granulocyte Colony-Stimulating Factor Promotes Preinvasive A...

Zhu W, Huang L, Li Y, Zhang X, Gu J, Yan Y, Xu X, Wang M, Qian H, Xu W. Cancer Lett. 2012 Feb 1;315(1):28-37. Doi: 10.1016/J.Canlet.2011.10.002. Epub 2011 Oct 14. Exosomes Derived From Human Bone Marrow Mesenchymal Stem Cells Promote Tumor Growth ...

Zhen-Qiang F, Bing-Wei Y, Yong-Liang L, Xiang-Wei W, Shan-Hong Y, Yuan-Ning Z, Wei-Sheng J, Wei C, Ye G. Genes Cells. 2012 Jan;17(1):53-64. Doi: 10.1111/J.1365-2443.2011.01572.X. Localized Expression Of Human Bmp-7 By Bm-Mscs Enhances Renal Repair...

Wang X, Li C, Zheng Y, Xia W, Yu Y, Ma X. Expert Opin Biol Ther. 2012 Sep;12(9):1129-39. Doi: 10.1517/14712598.2012.704016. Epub 2012 Jul 5. Bone Marrow Mesenchymal Stem Cells Increase Skin Regeneration Efficiency In Skin And Soft Tissue Expansion.

Zhang Xm, Du F, Yang D, Wang R, Yu Cj, Huang Xn, Hu Hy, Liu W, Fu J. Bmc Neurosci. 2011 Jun 24;12:61. Doi: 10.1186/1471-2202-12-61. Granulocyte Colony-Stimulating Factor Increases The Therapeutic Efficacy Of Bone Marrow Mononuclear Cell Transplant...

Liang Z, Liu J, Li L, Wang H, Zhao C, Jiang L, Qin G. Am J Otolaryngol. 2014 Mar-Apr;35(2):120-9. Doi: 10.1016/J.Amjoto.2013.10.009. Epub 2013 Oct 30. Effect Of Nimesulide On The Growth Of Human Laryngeal Squamous Cell Carcinoma.

Zhang Xm, Du F, Yang D, Yu Cj, Huang Xn, Liu W, Fu J. Bmc Neurosci. 2010 Oct 25;11:138. Doi: 10.1186/1471-2202-11-138. Transplanted Bone Marrow Stem Cells Relocate To Infarct Penumbra And Co-Express Endogenous Proliferative And Immature Neuronal M...

Shizhu J, Xiangwei M, Xun S, Mingzi H, Bingrong L, Dexia K, Xinghong W, Fenghua P. Turk J Gastroenterol. 2012 Aug;23(4):344-52. Bone Marrow Mononuclear Cell Transplant Therapy In Mice With Ccl4-Induced Acute Liver Failure.

Yu Z, Wang F, Liang N, Wang C, Peng X, Fang J, Cui H, Jameel Mughal M, Lai W. Biol Trace Elem Res. 2015 Nov;168(1):242-51. Doi: 10.1007/S12011-015-0344-1. Epub 2015 May 2. Effect Of Selenium Supplementation On Apoptosis And Cell Cycle Blockage Of ...

Zhang S, Peng X, Fang J, Cui H, Zuo Z, Chen Z. Biol Trace Elem Res. 2014 Jul;160(1):32-40. Doi: 10.1007/S12011-014-0009-5. Epub 2014 Jun 1. Effects Of Aflatoxin B1 Exposure And Sodium Selenite Supplementation On The Histology, Cell Proliferation, ...

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17 Customer Q&As for Anti-PCNA Antibody (Monoclonal, PC 10)

Question

Is this MA1083 anti-PCNA antibody (Monoclonal, PC 10) reactive to the isotypes of PCNA?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

The immunogen of MA1083 anti-PCNA antibody (Monoclonal, PC 10) is Protein A fusion protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-03

Question

We have seen staining in rat bone marrow lung. Are there any suggestions? Is anti-PCNA antibody (Monoclonal, PC 10) supposed to stain bone marrow lung positively?

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

From literature bone marrow lung does express PCNA. From Uniprot.org, PCNA is expressed in oocyte, skeletal muscle, bone marrow lung, fetal brain cortex, placenta, liver, among other tissues. Regarding which tissues have PCNA expression, here are a few articles citing expression in various tissues:
Bone marrow, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 9305916
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 12522211
Skeletal muscle, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-01-16

Question

Does anti-PCNA antibody (Monoclonal, PC 10) MA1083 work for WB with oocyte?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

According to the expression profile of oocyte, PCNA is highly expressed in oocyte. So, it is likely that anti-PCNA antibody (Monoclonal, PC 10) MA1083 will work for WB with oocyte.

Boster Scientific Support

Answered: 2019-12-11

Question

Please see the WB image, lot number and protocol we used for oocyte using anti-PCNA antibody (Monoclonal, PC 10) MA1083. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-16

Question

I am looking for to test anti-PCNA antibody (Monoclonal, PC 10) MA1083 on human oocyte for research purposes, then I may be interested in using anti-PCNA antibody (Monoclonal, PC 10) MA1083 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

The products we sell, including anti-PCNA antibody (Monoclonal, PC 10) MA1083, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-16

Question

I was wanting to use your anti-PCNA antibody (Monoclonal, PC 10) for WB for human oocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human oocyte identification?

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

As indicated on the product datasheet, MA1083 anti-PCNA antibody (Monoclonal, PC 10) has been validated for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human oocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-06

Question

Do you have a BSA free version of anti-PCNA antibody (Monoclonal, PC 10) MA1083 available?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PCNA antibody (Monoclonal, PC 10) MA1083 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-12

Question

I was wanting to use using your anti-PCNA antibody (Monoclonal, PC 10) for liver regeneration studies. Has this antibody been tested with western blotting on human hela? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

Thank you for your inquiry. This MA1083 anti-PCNA antibody (Monoclonal, PC 10) is validated on human hela, jurkat whole cell lysates. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-21

Question

Is a blocking peptide available for product anti-PCNA antibody (Monoclonal, PC 10) (MA1083)?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

We do provide the blocking peptide for product anti-PCNA antibody (Monoclonal, PC 10) (MA1083). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-21

Question

Would anti-PCNA antibody (Monoclonal, PC 10) MA1083 work on primate IHC with skeletal muscle?

Verified Customer

Verified customer

Asked: 2018-03-19

Answer

Our lab technicians have not tested anti-PCNA antibody (Monoclonal, PC 10) MA1083 on primate. You can run a BLAST between primate and the immunogen sequence of anti-PCNA antibody (Monoclonal, PC 10) MA1083 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate skeletal muscle in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-03-19

Question

I see that the anti-PCNA antibody (Monoclonal, PC 10) MA1083 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-07-10

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-07-10

Question

We are currently using anti-PCNA antibody (Monoclonal, PC 10) MA1083 for rat tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2017-05-26

Answer

The anti-PCNA antibody (Monoclonal, PC 10) (MA1083) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-05-26

Question

My team were satisfied with the WB result of your anti-PCNA antibody (Monoclonal, PC 10). However we have been able to see positive staining in skeletal muscle nucleus using this antibody. Is that expected? Could you tell me where is PCNA supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-05-12

Answer

According to literature, skeletal muscle does express PCNA. Generally PCNA expresses in nucleus. Regarding which tissues have PCNA expression, here are a few articles citing expression in various tissues:
Bone marrow, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 9305916
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 12522211
Skeletal muscle, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2017-05-12

Question

My question regarding product MA1083, anti-PCNA antibody (Monoclonal, PC 10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

N. Banerjee

Verified customer

Asked: 2017-01-04

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1083 anti-PCNA antibody (Monoclonal, PC 10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-01-04

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for oocyte using anti-PCNA antibody (Monoclonal, PC 10) MA1083. Let me know if you need anything else.

K. Moore

Verified customer

Asked: 2016-05-09

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-05-09

Question

We purchased anti-PCNA antibody (Monoclonal, PC 10) for IHC on skeletal muscle a few years ago. I am using mouse, and We intend to use the antibody for ICC next. My question regards examining skeletal muscle as well as oocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?

G. Huang

Verified customer

Asked: 2014-08-29

Answer

I looked at the website and datasheets of our anti-PCNA antibody (Monoclonal, PC 10) and I see that MA1083 has been tested on mouse in both IHC and ICC. Thus MA1083 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-08-29

Question

Will MA1083 anti-PCNA antibody (Monoclonal, PC 10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Singh

Verified customer

Asked: 2013-03-04

Answer

It shows on the product datasheet, MA1083 anti-PCNA antibody (Monoclonal, PC 10) as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-03-04

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MA1083
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