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Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clone Number:PC 10
Application:IHC, ICC, WB
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Product Name Anti-PCNA Antibody (Monoclonal, PC 10)
SKU/Catalog Number MA1083
Description Mouse IgG monoclonal antibody for PCNA, proliferating cell nuclear antigen (PCNA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins.
Cite This Product Anti-PCNA Antibody (Monoclonal, PC 10) PC 10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1083)
Replacement Item This antibody may replace the following items: sc-65598|sc-56|sc-9857|sc-71858|sc-27912|sc-33756|sc-12849|sc-34005|sc-53408|sc-53407|sc-7907|sc-25280|sc-9857-R from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG2a
Validated Species Human, Mouse, Rat
Predicted Species Bovine

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Protein A fusion protein.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Monoclonal
Clone Number PC 10
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG2a

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Pcna
Protein Name Proliferating cell nuclear antigen
Molecular Weight 28749 MW
Protein Function Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity). .
Sequence Similarities Belongs to the PCNA family.
Subcellular Localization Nucleus . Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (By similarity). .
Uniprot ID P04961
Alternative Names Proliferating cell nuclear antigen;PCNA;Cyclin;Pcna;
Research Areas |cell biology|cell cycle|markers| epigenetics and nuclear signaling|dna methylation|methylated dna binding| tags & cell markers|cell type markers|replication|dna / rna|dna synthesis|chromatin binding proteins|dna / rna binding| cancer|cell division|tumor biomarkers|tumor antigens| isotype/loading controls|pcna|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Proliferating cell nuclear antigen

Proliferating cell nuclear antigen(PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.

Anti-PCNA Antibody (Monoclonal, PC 10) Images

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Anti-PCNA Antibody (Monoclonal, PC 10)
Anti-PCNA antibody (monoclonal), MA1083, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 4: COLO320 Cell Lysate
Anti-PCNA Antibody (Monoclonal, PC 10)
Anti-PCNA antibody (monoclonal), MA1083, IHC(P)
IHC(P): Human Rectal Cancer Tissue
Anti-PCNA Antibody (Monoclonal, PC 10)
Anti-PCNA antibody (monoclonal), MA1083, ICC
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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to pcna antibody, cyclin antibody, proliferating cell nuclear antigen antibody