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Product Info Summary
| SKU: | A01263-Biotin |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Chicken, Drosophila, Human, Mouse, Rabbit, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-beta-Actin ACTB Antibody (biotin)
SKU/Catalog Number
A01263-Biotin
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-beta-Actin ACTB Antibody (biotin) (Catalog # A01263-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Chicken, Drosophila.
Storage & Handling
Biotin-Beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-beta-Actin ACTB Antibody (biotin) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01263-Biotin)
Host
Rabbit
Contents
Biotin-Beta-Actin antibody is supplied in PBS containing 1% stabilizing protein and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Biotin-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin.
Cross-reactivity
Biotin-Beta-Actin antibody is human, mouse, rat, rabbit, chicken, and drosophila reactive.
Reactive Species
A01263-Biotin is reactive to ACTB in Chicken, Drosophila, Human, Mouse, Rabbit, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
41.7 kDa
Background of ACTB
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01263-Biotin is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Biotin-Beta-Actin antibody can be used for detection of Beta-Actin by Western blot at 0.5 - 1 μ/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Actin in 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT4, 3T3, Raji, THP-1, mouse brain, rat brain, rabbit brain, mouse lung, rat lung, mouse liver, rat liver, rabbit spleen, chicken small intestine, and drosophila lysate with Biotin-Beta-Actin antibody at 1 μg/mL.
Specific Publications For Anti-beta-Actin ACTB Antibody (biotin) (A01263-Biotin)
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