Anti-PD-L1/CD274 Antibody Picoband™

PD-L1 antibody

Boster Bio Anti-PD-L1/CD274 Antibody Picoband™ catalog # PB9994. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PB9994
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-PD-L1/CD274 Antibody Picoband™

View all PD-L1 Antibodies

SKU/Catalog Number

PB9994

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PD-L1/CD274 Antibody Picoband™ catalog # PB9994. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PD-L1/CD274 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9994)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse PD-L1 recombinant protein (Position: F19-T238). Mouse PD-L1 shares 78.6% amino acid (aa) sequence identity with human PD-L1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9994 is reactive to CD274 in Mouse, Rat

Applications

PB9994 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

33.275kDa

Background of PD-L1

Programmed death-ligand 1 (PD-L1), also known as CD274 or B7-H1, is a protein that in humans is encoded by the CD274 gene. It is mapped to 9p24.1. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. It has been concluded that upregulation of PD-L1 on tumor MDCs downregulates T-cell immunity and that PD-L1 blockade may represent an approach for cancer immunotherapy. Additionally, PD-L1 can provide positive costimulatory signals for innate and adaptive immunity and for protection against intracellular bacterial infection. What’s more, it has been found that PD1/PDL1 pathway may be a good target for restoring antitumor immunity in ovarian cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For CD274 (Source: Uniprot.org, NCBI)

Gene Name

CD274

Full Name

Programmed cell death 1 ligand 1

Weight

33.275kDa

Superfamily

immunoglobulin superfamily

Alternative Names

B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1 CD274 B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 CD274 molecule programmed cell death 1 ligand 1|B7 homolog 1|CD274 antigen|PDCD1 ligand 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD274, check out the CD274 Infographic

CD274 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD274: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9994 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

CD25 and TGF-%u03B2 blockade based on predictive integrated immune ratio inhibits tumor growth in pancreatic cancer

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6 Customer Q&As for Anti-PD-L1/CD274 Antibody Picoband™

Question

We have been able to see staining in mouse chorionic villus. Any tips? Is anti-PD-L1/CD274 antibody supposed to stain chorionic villus positively?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

From literature chorionic villus does express CD274. From Uniprot.org, CD274 is expressed in chorionic villus, placenta, gastric carcinoma, lung, liver, among other tissues. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039

Boster Scientific Support

Answered: 2019-11-29

Question

We are currently using anti-PD-L1/CD274 antibody PB9994 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

The anti-PD-L1/CD274 antibody (PB9994) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-13

Question

We are currently using anti-PD-L1/CD274 antibody PB9994 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-07-31

Answer

The anti-PD-L1/CD274 antibody (PB9994) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-07-31

Question

We purchased anti-PD-L1/CD274 antibody for ELISA on gastric carcinoma in the past. I am using mouse, and We want to use the antibody for WB next. I am interested in examining gastric carcinoma as well as lung in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-07-04

Answer

I viewed the website and datasheets of our anti-PD-L1/CD274 antibody and it seems that PB9994 has been validated on mouse in both ELISA and WB. Thus PB9994 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-07-04

Question

Our team were well pleased with the WB result of your anti-PD-L1/CD274 antibody. However we have observed positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is CD274 supposed to be expressed?

P. Mitchell

Verified customer

Asked: 2014-05-26

Answer

From literature, liver does express CD274. Generally CD274 expresses in cell membrane. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039

Boster Scientific Support

Answered: 2014-05-26

Question

I am looking for using your anti-PD-L1/CD274 antibody for immune response studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.

C. Mitchell

Verified customer

Asked: 2013-03-14

Answer

We appreciate your inquiry. This PB9994 anti-PD-L1/CD274 antibody is validated on mouse brain. It is guaranteed to work for ELISA, WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-03-14

Order DetailsPrice
PB9994

100μg

$370
PB9994-10ug

10μg sample (liquid)

$99
PB9994-Biotin

100 μg Biotin conjugated

$570
PB9994-Cy3

100 μg Cy3 conjugated

$570
PB9994-Dylight488

100 μg Dylight488 conjugated

$570
PB9994-Dylight550

100 μg Dylight550 conjugated

$570
PB9994-Dylight594

100 μg Dylight594 conjugated

$570
PB9994-FITC

100 μg FITC conjugated

$570
PB9994-HRP

100 μg HRP conjugated

$570
PB9994-APC

100 μg APC conjugated

$670
PB9994-PE

100 μg PE conjugated

$670
PB9994-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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