Anti-Pdcd4 phospho S457 Monoclonal Antibody

Product Name

Anti-Pdcd4 phospho S457 Monoclonal Antibody

View all PDCD4 Antibodies

SKU/Catalog Number

MP01105-1

Size

50ug

Form

Lyophilized

Description

Boster Bio Anti-Pdcd4 phospho S457 Monoclonal Antibody (Catalog # MP01105-1). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Xenopus.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)

Cite This Product

Anti-Pdcd4 phospho S457 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP01105-1)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, antibody formulated with stabilizing protein (10 mg/mL) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

Clone: 9G6

Isotype

IgG1 kappa

Immunogen

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.

Reactive Species

MP01105-1 is reactive to PDCD4 in Human, Mouse, Rat, Xenopus

Observed Molecular Weight

68 kDa

Calculated molecular weight

51.7 kDa

Background of PDCD4

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

MP01105-1 is guaranteed for ELISA, IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:100,000
IP: 1:1,000 - 1:5,000
WB: 1:2,000 - 1:10,000
Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody Biotin Conjugated has been tested by ELISA and western blotting and is suitable for immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Validation Images & Assay Conditions

See full target information PDCD4

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis