|Validated Species:||Human, Mouse, Rat|
|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-PDK2 Antibody|
|Description||Rabbit IgG polyclonal antibody for [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial (PDK2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-PDK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1994)|
|Replacement Item||This antibody may replace the following items: sc-14484|sc-14486|sc-100534 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human PDK2(379-392aa WRHYQTIQEAGDWC), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial|
|Molecular Weight||46154 MW|
|Protein Function||Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis. .|
|Tissue Specificity||Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung. .|
|Sequence Similarities||Belongs to the PDK/BCKDK protein kinase family.|
|Subcellular Localization||Mitochondrion matrix.|
|Alternative Names||[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial;220.127.116.11;Pyruvate dehydrogenase kinase isoform 2;PDH kinase 2;PDKII;PDK2;PDHK2;|
|Research Areas|||signal transduction|metabolism|energy metabolism| signal transduction|mitochondrial| cancer|cancer metabolism|metabolic signaling pathway|integration of energy metabolism| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial markers|metabolic signaling pathways|energy transfer pathways|integration of energy||
Background for [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-PDK2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-PDK2 Antibody Images
Click the images to enlarge.
Lane 1: Rat Heart Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: MCF-7 Cell Lysate
IHC(P): Human Mammary Cancer Tissue
IHC(P): Rat Brain Tissue
ICC: HELA Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,