Human Resistin ELISA Kit PicoKine™

Human Resistin ELISA Kit PicoKine™ (96 Tests). Quantitate Human RETN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK0581
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Resistin ELISA Kit PicoKine™

See all Resistin products

SKU/Catalog Number

EK0581

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Resistin ELISA Kit PicoKine™ (96 Tests). Quantitate Human RETN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Resistin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0581)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: K19-P108

Sensitivity

<3 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0581 is reactive to RETN in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0581 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0581-CAP Anti-Human RETN Pre-coated 96-well strip microplate 1
EK0581-ST Human RETN Standard 2 vials, 10 ng/tube
EK0581-DA Human RETN Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156312625125025005000
O.D.0.0000.1210.2080.3670.6600.9891.4521.990

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Resistin, Dilution ratio of 1:10, concentration in serum is around 20ng/ml.

Some articles we found to cite concentrations of Resistin in samples: 23400230 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Resistin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)13563920671426362227
Standard deviation8.140.25161.228.5246.42177.83
CV (%)6%7.3%7.8%6%7.3%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Resistin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11351371431461404.433.1%
Sample 263965363473766541.726.2%
Sample 320672270233723372252110.674.9%
*number of samples for each test n=16.

Gene/Protein Information For RETN (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9HD89

Gene ID

56729

Gene Name

RETN

Full Name

Resistin

Weight

11.419kDa

Superfamily

resistin/FIZZ family

Alternative Names

Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1 RETN ADSF, FIZZ31, RSTN, XCP1, RETN resistin resistin|C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1|adipose tissue-specific secretory factor|c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein|cysteine-rich secreted protein A12-alpha-like 2|cysteine-rich secreted protein FIZZ3|found in inflammatory zone 3|resistin delta2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RETN, check out the RETN Infographic

RETN infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for RETN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0581 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Adipocytokine concentrations in children with different types of beta-thalassemia

Obesity, Serum Resistin and Leptin Levels Linked to Coronary Artery Disease

Niu XH, Li L, Li JY, Song Q, Jin MM, Liu JX. Medicine (Baltimore). 2017 Apr;96(16):e6622. doi: 10.1097/MD.0000000000006622. Serum resistin positively correlates with serum lipids, but not with insulin resistance, in first-degree relatives of type-...

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9 Customer Q&As for Human Resistin ELISA Kit PicoKine™

Question

Q: how to thaw whole blood sample for Resistin ELISA after freezing?

R. Edwards

Verified customer

Asked: 2020-09-10

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Resistin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-09-10

Question

Q: can you suggest the dilution ratio of serum samples for detection of Resistin in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

H. Anand

Verified customer

Asked: 2020-08-12

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Resistin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-08-12

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

A. Rodriguez

Verified customer

Asked: 2020-08-03

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-08-03

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

W. Wilson

Verified customer

Asked: 2020-08-02

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-08-02

Question

Q: how to proceed with the analysis of ELISA data? I measured Resistin level in plasma.

M. Jha

Verified customer

Asked: 2020-03-04

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-03-04

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-08-22

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Resistin using the EK0581 Human Resistin Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-12-30

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2018-12-30

Question

Q: is it okay to use citrate plasma as samples in Human Resistin Picokine® ELISA Kit (Catalog # EK0581)?

Verified Customer

Verified customer

Asked: 2018-11-07

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Resistin causing degradation of its protein structure. Resistin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Resistin ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-11-07

Question

What is the reference range of this kit in human serum?

Verified Customer

Verified customer

Asked: 2014-03-28

Answer

The reference range of this kit in human serum is 5-40ng/mL.

Boster Scientific Support

Answered: 2014-03-28

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