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Product Info Summary
| SKU: | PB9412 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Resistin/RETN Antibody Picoband®
SKU/Catalog Number
PB9412
PB0427 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Resistin/RETN Antibody Picoband® catalog # PB9412. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Resistin/RETN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9412)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Resistin recombinant protein (Position: K19-P108). Human Resistin shares 58.6% amino acid (aa) sequence identity with mouse Resistin.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9412 is reactive to RETN in Human
Observed Molecular Weight
11 kDa
Calculated molecular weight
11.4 kDa
Background of RETN
Resistin, also known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1) is a cysteine-rich protein that in humans is encoded by the RETN gene. The resistin gene comprises 4 exons, the first of which is untranslated, and spans approximately 1,750 bp. The human resistin gene is localized to a cloned fragment of human chromosome 19. In primates, pigs and dogs, resistin is secreted by immune and epithelial cells while in rodents, it is secreted by adipose tissue. Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9412 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human , -
ELISA, 0.1-0.5μg/ml, -
Validation Images & Assay Conditions
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Western blot analysis of Resistin using anti-Resistin antibody (PB9412).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Human Resistin Protein 0.5ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Resistin antigen affinity purified polyclonal antibody (Catalog # PB9412) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Resistin at approximately 11 kDa. The expected band size for Resistin is at 11 kDa.
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Monomethyl fumarate (MMF) fully suppresses differentiation mixture (MIX)-induced protein expression of the transcription factors peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBP-α) (a) as well as the adipokines, resistin and adiponectin (b). Cellular protein abundance was assessed by Western blotting. Data are expressed as the means ± standard deviation; * p < 0.05 and ** p < 0.01.
Index in PubMed under a CC BY license. PMID: 39765824
Specific Publications For Anti-Resistin/RETN Antibody Picoband® (PB9412)
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4 Customer Q&As for Anti-Resistin/RETN Antibody Picoband®
Question
Does anti-Resistin/RETN antibody PB9412 work on bovine ELISA with bone marrow?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
Our lab technicians have not validated anti-Resistin/RETN antibody PB9412 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Resistin/RETN antibody PB9412 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine bone marrow in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-18
Question
We are currently using anti-Resistin/RETN antibody PB9412 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
The anti-Resistin/RETN antibody (PB9412) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-06
Question
I have a question about product PB9412, anti-Resistin/RETN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9412 anti-Resistin/RETN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-17
Question
I am interested in to test anti-Resistin/RETN antibody PB9412 on human brain for research purposes, then I may be interested in using anti-Resistin/RETN antibody PB9412 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Zhao
Verified customer
Asked: 2013-10-18
Answer
The products we sell, including anti-Resistin/RETN antibody PB9412, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-10-18
