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Product Info Summary
| SKU: | M06683-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-PDXK Mouse Monoclonal Antibody [Clone ID: OTI5H5]
SKU/Catalog Number
M06683-1
Size
100 µl
Description
Boster Bio PDXK mouse monoclonal antibody, clone OTI5H5 (formerly 5H5). Catalog# M06683-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PDXK Mouse Monoclonal Antibody [Clone ID: OTI5H5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06683-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI5H5
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human PDXK (NP_03672) produced in HEK293 cell.
Reactive Species
M06683-1 is reactive to PDXK in Human, Mouse, Rat
Calculated molecular weight
35.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06683-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:150
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDXK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDXK.
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Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-PDXK monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either PDXK (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDXK antibody (M06683-1)
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Anti-PDXK mouse monoclonal antibody (M06683-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDXK.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT) and PDXK-Knockout HEK293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PDXK monoclonal antibody M06683-1 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Specific Publications For Anti-PDXK Mouse Monoclonal Antibody [Clone ID: OTI5H5] (M06683-1)
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